Restriction map for
pF1KE1823
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 37 GTATAC GT^[AC][GT]AC
AciI 660 CCGC CCGC(-3/-1)
AflIII 95 ACATGT A^C[GA][CT]GT
AgeI 916 ACCGGT A^CCGGT
AlwI 501 GATCC GGATC(4/5)
AspHI 44 GAGCTC G[AT]GC[AT]^C
AvaI 648 CTCGGG C^[CT]CG[GA]G
BanI 885 GGCACC G^G[CT][GA]CC
BanII 44 GAGCTC G[GA]GC[CT]^C
BglII 852 AGATCT A^GATCT
BmyI 44 GAGCTC G[AGT]GC[ACT]^C
BpmI 761 CTGGAG CTGGAG(16/14)
BsaWI 916 ACCGGT [AT]^CCGG[AT]
BsgI 478 GTGCAG GTGCAG(16/14)
BsiHKAI 44 GAGCTC G[AT]GC[AT]^C
BsmI 288 GAATGC GAATGC(1/-1)
BsoBI 648 CTCGGG C^[CT]CG[GA]G
Bsp1286I 44 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 95 ACATGT A^CATGT
BsrFI 916 ACCGGT [GA]^CCGG[CT]
Bst1107I 37 GTATAC GTA^TAC
BstZ17I 37 GTATAC GTA^TAC
CfoI 680 GCGC GCG^C
Cfr10I 916 ACCGGT [GA]^CCGG[CT]
DraII 160 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 160 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 246 GAATTC G^AATTC
HapII 917 CCGG C^CGG
HgaI 614 GCGTC GACGC(5/10)
HhaI 680 GCGC GCG^C
HinP1I 680 GCGC G^CGC
HpaII 917 CCGG C^CGG
MaeII 469 ACGT A^CGT
MspI 917 CCGG C^CGG
MwoI 590 GCAACTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 95 ACATGT [GA]CATG^[CT]
PinAI 916 ACCGGT A^CCGGT
PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT]
PstI 594 CTGCAG CTGCA^G
PvuII 395 CAGCTG CAG^CTG
SacI 44 GAGCTC GAGCT^C
SapI 25 GCTCTTC GCTCTTC(1/4)
SfaNI 128 GCATC GCATC(5/9)
TaqI 518 TCGA T^CGA
TfiI 172 GATTC G^A[AT]TC
TspRI 485 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 518 TCGA T^CGA
XbaI 102 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 246 GAATTC [GA]^AATT[CT]
555 AAATTT
AluI 45 AGCT AG^CT
396 AGCT
ApoI 246 GAATTC [GA]^AATT[CT]
555 AAATTT
AvaII 161 GGACC G^G[AT]CC
543 GGTCC
BciVI 873 GGATAC GTATCC(6/5)
114 GTATCC
BfaI 103 CTAG C^TAG
1001 CTAG
BsmAI 99 GTCTC GTCTC(1/5)
640 GTCTC
BssKI 430 CCTGG ^CC[ACGT]GG
869 CCTGG
BstF5I 624 CATCC GGATG(2/0)
942 GGATG
BstNI 430 CCTGG CC^[AT]GG
869 CCTGG
BstYI 500 AGATCC [GA]^GATC[CT]
852 AGATCT
Cfr13I 161 GGACC G^G[ACGT]CC
543 GGTCC
EcoRII 430 CCTGG ^CC[AT]GG
869 CCTGG
EcoT14I 546 CCATGG C^C[AT][AT]GG
938 CCATGG
FokI 624 CATCC GGATG(9/13)
942 GGATG
HaeIII 473 GGCC GG^CC
675 GGCC
HincII 920 GTCAAC GT[CT]^[GA]AC
967 GTCAAC
HindII 920 GTCAAC GT[CT]^[GA]AC
967 GTCAAC
HphI 360 GGTGA GGTGA(8/7)
560 TCACC
MaeI 103 CTAG C^TAG
1001 CTAG
MaeIII 442 GTCAC ^GT[ACGT]AC
634 GTCAC
MflI 500 AGATCC [GA]^GATC[CT]
852 AGATCT
MspA1I 395 CAGCTG C[AC]G^C[GT]G
660 CCGCTG
MvaI 430 CCTGG CC^[AT]GG
869 CCTGG
NcoI 546 CCATGG C^CATGG
938 CCATGG
Sau96I 161 GGACC G^G[ACGT]CC
543 GGTCC
ScrFI 430 CCTGG CC^[ACGT]GG
869 CCTGG
StyI 546 CCATGG C^C[AT][AT]GG
938 CCATGG
Tsp45I 442 GTCAC ^GT[GC]AC
634 GTCAC
XcmI 340 CCACAGGGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
929 CCAATCAATCCATGG
XhoII 500 AGATCC [GA]^GATC[CT]
852 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BsmFI
Cac8I DdeI DpnII DsaI EarI
Fnu4HI HinfI ItaI Ksp632I MboI
MboII MnlI MseI NdeII NlaIII
NlaIV PleI Sau3AI SfcI Tru9I
TseI Tsp509I