Restriction map for
pF1KE3344
##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AvrII BclI BfrI BglII BlnI
BsaWI BseAI BsiEI BspDI BspEI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI TfiI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 295 GTCTAC GT^[AC][GT]AC
AhdI 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2265 GTGCAC G^TGCAC
AocI 750 CCTGAGG CC^T[ACGT]AGG
ApaLI 2265 GTGCAC G^TGCAC
AspEI 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1196 TGCGCA TGC^GCA
BalI 2054 TGGCCA TGG^CCA
BamHI 1691 GGATCC G^GATCC
BbeI 2886 GGCGCC GGCGC^C
BbrPI 2406 CACGTG CAC^GTG
BbsI 231 GAAGAC GAAGAC(2/6)
BbvCI 573 GCTGAGG CCTCAGC(-5/-2)
BcgI 489 CGAGACCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2181 GGATAC GTATCC(6/5)
BfaI 1582 CTAG C^TAG
BpuAI 231 GAAGAC GAAGAC(2/6)
BsaAI 2406 CACGTG [CT]AC^GT[GA]
BsaBI 2745 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 90 CGTACG C^GTACG
BsmI 1041 GAATGC GAATGC(1/-1)
Bsp1407I 1451 TGTACA T^GTACA
BsrGI 1451 TGTACA T^GTACA
BssSI 2494 CTCGTG CACGAG(-5/-1)
BstAPI 690 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2384 CCAGCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
DraIII 2155 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 766 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
FspI 1196 TGCGCA TGC^GCA
KasI 2886 GGCGCC G^GCGCC
MaeI 1582 CTAG C^TAG
MamI 2745 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2054 TGGCCA TGG^CCA
MscI 2054 TGGCCA TGG^CCA
MseI 2552 TTAA T^TAA
NarI 2886 GGCGCC GG^CGCC
NcoI 2057 CCATGG C^CATGG
PaeR7I 236 CTCGAG C^TCGAG
PmaCI 2406 CACGTG CAC^GTG
PmlI 2406 CACGTG CAC^GTG
SacI 1576 GAGCTC GAGCT^C
SexAI 1181 ACCAGGT A^CC[AT]GGT
SfoI 2886 GGCGCC GGC^GCC
SspBI 1451 TGTACA T^GTACA
StuI 2594 AGGCCT AGG^CCT
Tru9I 2552 TTAA T^TAA
Tsp509I 734 AATT ^AATT
XhoI 236 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 292 GACGTC GACGT^C
2710 GACGTC
AspI 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACAGTGTC
BglI 199 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2008 GCCAAAGTGGC
BsmBI 2772 CGTCTC CGTCTC(1/5)
1319 GAGACG
EarI 970 GAAGAG CTCTTC(1/4)
1002 GAAGAG
EcoT22I 1300 ATGCAT ATGCA^T
1730 ATGCAT
HaeII 196 AGCGCC [GA]GCGC^[CT]
2886 GGCGCC
HgaI 1661 GCGTC GACGC(5/10)
1751 GCGTC
Ksp632I 970 GAAGAG CTCTTC(1/4)
1002 GAAGAG
MslI 559 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2433 CACCCTGGTG
NsiI 1300 ATGCAT ATGCA^T
1730 ATGCAT
NspI 1302 GCATGC [GA]CATG^[CT]
2148 GCATGC
PflFI 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACAGTGTC
PpuMI 1997 AGGACCT [GA]G^G[AT]CC[CT]
2102 AGGTCCT
SbfI 402 CCTGCAGG CCTGCA^GG
663 CCTGCAGG
ScaI 962 AGTACT AGT^ACT
2570 AGTACT
SphI 1302 GCATGC GCATG^C
2148 GCATGC
Sse8387I 402 CCTGCAGG CCTGCA^GG
663 CCTGCAGG
Tth111I 1180 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACAGTGTC
XcmI 254 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1388 CCAGCCGCGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI