Restriction map for
pF1KE2389
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT14I
FauI FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 1870 GTCTAC GT^[AC][GT]AC
AspHI 1739 GAGCAC G[AT]GC[AT]^C
BamHI 262 GGATCC G^GATCC
BanI 1654 GGCACC G^G[CT][GA]CC
BanII 1775 GGGCTC G[GA]GC[CT]^C
BbrPI 1826 CACGTG CAC^GTG
BglII 720 AGATCT A^GATCT
BpmI 1534 CTCCAG CTGGAG(16/14)
BsaI 1531 GGTCTC GGTCTC(1/5)
BsgI 1643 CTGCAC GTGCAG(16/14)
BsiHKAI 1739 GAGCAC G[AT]GC[AT]^C
BsmFI 2038 GTCCC GGGAC(10/14)
Bsp1407I 1588 TGTACA T^GTACA
BspMI 1459 GCAGGT ACCTGC(4/8)
BsrGI 1588 TGTACA T^GTACA
BssKI 675 CCTGG ^CC[ACGT]GG
BstNI 675 CCTGG CC^[AT]GG
DraIII 125 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 186 CTCTTC CTCTTC(1/4)
EcoRI 1751 GAATTC G^AATTC
EcoRII 675 CCTGG ^CC[AT]GG
EcoT22I 1970 ATGCAT ATGCA^T
HapII 1307 CCGG C^CGG
HpaII 1307 CCGG C^CGG
Ksp632I 186 CTCTTC CTCTTC(1/4)
MfeI 1389 CAATTG C^AATTG
MspI 1307 CCGG C^CGG
MunI 1389 CAATTG C^AATTG
MvaI 675 CCTGG CC^[AT]GG
NsiI 1970 ATGCAT ATGCA^T
PacI 427 TTAATTAA TTAAT^TAA
PmaCI 1826 CACGTG CAC^GTG
PmlI 1826 CACGTG CAC^GTG
PstI 1509 CTGCAG CTGCA^G
ScrFI 675 CCTGG CC^[ACGT]GG
SnaBI 1084 TACGTA TAC^GTA
SspBI 1588 TGTACA T^GTACA
SspI 949 AATATT AAT^ATT
XcmI 560 CCATAGTACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AseI 430 ATTAAT AT^TAAT
1926 ATTAAT
AsnI 430 ATTAAT AT^TAAT
1926 ATTAAT
Asp700I 81 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
869 GAAAAGCTTC
BbsI 1075 GTCTTC GAAGAC(2/6)
1884 GTCTTC
BbvI 94 GCAGC GCAGC(8/12)
1049 GCAGC
BmyI 1739 GAGCAC G[AGT]GC[ACT]^C
1775 GGGCTC
BpuAI 1075 GTCTTC GAAGAC(2/6)
1884 GTCTTC
BsaJI 674 CCCTGG C^C[ACGT][ACGT]GG
675 CCTGGG
BsmI 636 GAATGC GAATGC(1/-1)
1567 GAATGC
Bsp1286I 1739 GAGCAC G[AGT]GC[ACT]^C
1775 GGGCTC
BspLU11I 506 ACATGT A^CATGT
1662 ACATGT
BsrDI 71 GCAATG GCAATG(2/0)
646 GCAATG
BstF5I 993 CATCC GGATG(2/0)
1995 GGATG
DraI 486 TTTAAA TTT^AAA
985 TTTAAA
EcoRV 180 GATATC GAT^ATC
1963 GATATC
Fnu4HI 94 GCAGC GC^[ACGT]GC
1049 GCAGC
FokI 993 CATCC GGATG(9/13)
1995 GGATG
HindIII 213 AAGCTT A^AGCTT
872 AAGCTT
ItaI 94 GCAGC GC^[ACGT]GC
1049 GCAGC
PshBI 430 ATTAAT AT^TAAT
1926 ATTAAT
SfcI 1493 CTATAG C^T[GA][CT]AG
1509 CTGCAG
TaqI 409 TCGA T^CGA
756 TCGA
TseI 94 GCAGC G^C[AT]GC
1049 GCAGC
TthHB8I 409 TCGA T^CGA
756 TCGA
XmnI 81 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
869 GAAAAGCTTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AcsI AfaI AflIII AluI AlwI
ApoI AvaII BfaI BsaAI BsiYI
BslI BsmAI BsrI BstYI Cfr13I
DdeI DpnII HinfI HphI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I SfaNI TfiI Tru9I
Tsp45I Tsp509I TspRI Van91I XhoII