Restriction map for
pF1KE5061
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaJI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI HphI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 929 TCCGGA T^CCGGA
AciI 847 GCGG CCGC(-3/-1)
AflIII 29 ACACGT A^C[GA][CT]GT
AseI 373 ATTAAT AT^TAAT
AsnI 373 ATTAAT AT^TAAT
AvaII 732 GGACC G^G[AT]CC
BbsI 693 GAAGAC GAAGAC(2/6)
BbvCI 386 CCTCAGC CCTCAGC(-5/-2)
BbvI 844 GCAGC GCAGC(8/12)
BciVI 985 GTATCC GTATCC(6/5)
BcnI 327 CCCGG CC^[GC]GG
BfaI 124 CTAG C^TAG
BglII 542 AGATCT A^GATCT
BpuAI 693 GAAGAC GAAGAC(2/6)
BsaBI 738 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 929 TCCGGA [AT]^CCGG[AT]
BseAI 929 TCCGGA T^CCGGA
BseRI 949 GAGGAG GAGGAG(10/8)
BsmFI 943 GTCCC GGGAC(10/14)
BspEI 929 TCCGGA T^CCGGA
BspMI 919 ACCTGC ACCTGC(4/8)
BsrDI 161 GCAATG GCAATG(2/0)
BstXI 722 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 542 AGATCT [GA]^GATC[CT]
EcoRI 331 GAATTC G^AATTC
Fnu4HI 844 GCAGC GC^[ACGT]GC
HgaI 760 GACGC GACGC(5/10)
ItaI 844 GCAGC GC^[ACGT]GC
MaeI 124 CTAG C^TAG
MamI 738 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 542 AGATCT [GA]^GATC[CT]
MroI 929 TCCGGA T^CCGGA
MseI 374 TTAA T^TAA
MspA1I 845 CAGCGG C[AC]G^C[GT]G
NciI 327 CCCGG CC^[GC]GG
NlaIV 526 GGGGCC GG[ACGT]^[ACGT]CC
PshAI 696 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 373 ATTAAT AT^TAAT
SapI 651 GAAGAGC GCTCTTC(1/4)
SspI 370 AATATT AAT^ATT
TfiI 434 GATTC G^A[AT]TC
Tru9I 374 TTAA T^TAA
TseI 844 GCAGC G^C[AT]GC
XhoII 542 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 214 GTAC GT^AC
309 GTAC
BsiYI 260 CCTTCTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
945 CCCAGAGGAGG
BslI 260 CCTTCTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
945 CCCAGAGGAGG
BsmI 3 GAATGC GAATGC(1/-1)
102 GCATTC
BsrI 11 CCAGT ACTGG(1/-1)
911 CCAGT
BstNI 548 CCAGG CC^[AT]GG
1100 CCAGG
EarI 651 GAAGAG CTCTTC(1/4)
1107 GAAGAG
EcoRII 548 CCAGG ^CC[AT]GG
1100 CCAGG
HaeIII 528 GGCC GG^CC
551 GGCC
HapII 328 CCGG C^CGG
930 CCGG
HincII 45 GTTGAC GT[CT]^[GA]AC
757 GTTGAC
HindII 45 GTTGAC GT[CT]^[GA]AC
757 GTTGAC
HindIII 616 AAGCTT A^AGCTT
685 AAGCTT
HpaII 328 CCGG C^CGG
930 CCGG
Ksp632I 651 GAAGAG CTCTTC(1/4)
1107 GAAGAG
MslI 673 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1164 CACCAAAATG
MspI 328 CCGG C^CGG
930 CCGG
MvaI 548 CCAGG CC^[AT]GG
1100 CCAGG
MwoI 102 GCATTCTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
529 GCCATCTTAGC
PleI 411 GACTC GAGTC(4/5)
904 GAGTC
RsaI 214 GTAC GT^AC
309 GTAC
SmlI 223 CTCAAG C^T[CT][GA]AG
413 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AluI ApoI BclI BpmI
BsaI BsmAI BssKI BstF5I Cfr13I
DdeI DpnII FbaI FokI HinfI
MaeII MaeIII MboI MboII MnlI
NdeII NlaIII Sau3AI Sau96I ScrFI
Tsp45I Tsp509I TspRI