Restriction map for
pF1KB9766
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AseI
AsnI Asp700I Asp718I AviII AvrII
BbvCI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BssSI Bst1107I BstZ17I CelII ClaI
DrdI Eco47III EcoRI EcoRV EcoT22I
FseI FspI HpaI KpnI MroI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 1399 GGCGCGCC GG^CGCGCC
AspEI 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 887 TGGCCA TGG^CCA
BamHI 70 GGATCC G^GATCC
BcgI 687 CGAGGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2060 TGATCA T^GATCA
BsaBI 2291 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 635 CGTCTC CGTCTC(1/5)
Bsp1407I 1385 TGTACA T^GTACA
BspHI 491 TCATGA T^CATGA
BsrGI 1385 TGTACA T^GTACA
BstBI 2216 TTCGAA TT^CGAA
BstEII 405 GGTCACC G^GT[ACGT]ACC
BstPI 405 GGTCACC G^GT[ACGT]ACC
CpoI 499 CGGTCCG CG^G[AT]CCG
DraI 1915 TTTAAA TTT^AAA
EagI 1319 CGGCCG C^GGCCG
Eam1105I 226 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1319 CGGCCG C^GGCCG
Eco52I 1319 CGGCCG C^GGCCG
EcoO65I 405 GGTCACC G^GT[ACGT]ACC
FbaI 2060 TGATCA T^GATCA
HincII 1365 GTCGAC GT[CT]^[GA]AC
HindII 1365 GTCGAC GT[CT]^[GA]AC
HindIII 2428 AAGCTT A^AGCTT
MamI 2291 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2564 CAATTG C^AATTG
MluI 1115 ACGCGT A^CGCGT
MluNI 887 TGGCCA TGG^CCA
MscI 887 TGGCCA TGG^CCA
MunI 2564 CAATTG C^AATTG
NspI 1861 GCATGT [GA]CATG^[CT]
NspV 2216 TTCGAA TT^CGAA
PflFI 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 410 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 491 TCATGA T^CATGA
RsrII 499 CGGTCCG CG^G[AT]CCG
SalI 1365 GTCGAC G^TCGAC
SexAI 2028 ACCAGGT A^CC[AT]GGT
SfuI 2216 TTCGAA TT^CGAA
SmlI 1588 CTCAAG C^T[CT][GA]AG
SspBI 1385 TGTACA T^GTACA
StuI 1184 AGGCCT AGG^CCT
Tth111I 334 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 410 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 1365 GTCGAC GT^[AC][GT]AC
1609 GTCTAC
AcsI 1912 AAATTT [GA]^AATT[CT]
2710 GAATTT
AflIII 1115 ACGCGT A^C[GA][CT]GT
2307 ACACGT
AocI 362 CCTCAGG CC^T[ACGT]AGG
1632 CCTGAGG
ApaI 620 GGGCCC GGGCC^C
1416 GGGCCC
ApoI 1912 AAATTT [GA]^AATT[CT]
2710 GAATTT
BbrPI 37 CACGTG CAC^GTG
913 CACGTG
BbsI 973 GAAGAC GAAGAC(2/6)
1765 GTCTTC
BpuAI 973 GAAGAC GAAGAC(2/6)
1765 GTCTTC
BsaAI 37 CACGTG [CT]AC^GT[GA]
913 CACGTG
BsgI 1538 CTGCAC GTGCAG(16/14)
2741 CTGCAC
BsiEI 1055 CGGTCG CG[GA][CT]^CG
1319 CGGCCG
BspMI 97 GCAGGT ACCTGC(4/8)
1605 GCAGGT
BsrDI 2204 CATTGC GCAATG(2/0)
1780 GCAATG
BssHII 1192 GCGCGC G^CGCGC
1400 GCGCGC
BstAPI 579 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1948 GCACTGGATGC
BstXI 893 CCACGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2753 CCAAGATATTGG
Bsu36I 362 CCTCAGG CC^T[ACGT]AGG
1632 CCTGAGG
DraIII 1034 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2699 CACTGAGTG
EarI 175 CTCTTC CTCTTC(1/4)
2446 GAAGAG
Eco81I 362 CCTCAGG CC^T[ACGT]AGG
1632 CCTGAGG
EcoNI 1599 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2491 CCTTTGGAAGG
Ksp632I 175 CTCTTC CTCTTC(1/4)
2446 GAAGAG
PmaCI 37 CACGTG CAC^GTG
913 CACGTG
PmlI 37 CACGTG CAC^GTG
913 CACGTG
SacI 2551 GAGCTC GAGCT^C
2595 GAGCTC
SfcI 1061 CTGTAG C^T[GA][CT]AG
1128 CTATAG
XcmI 833 CCAGGGTCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
890 CCACCACGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI