Restriction map for
pF1KB5752
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AspHI
AspI AviII BalI BamHI BbeI
BbrPI BcgI BciVI BfrI BlpI
Bpu1102I BsaAI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HpaI KasI MluI MluNI
MscI NarI NdeI NheI NotI
NruI NsiI NspV PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SalI SapI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1990 GACGTC GACGT^C
Acc65I 422 GGTACC G^GTACC
AccI 853 GTAGAC GT^[AC][GT]AC
AccIII 1791 TCCGGA T^CCGGA
AcyI 1990 GACGTC G[GA]^CG[CT]C
AhdI 814 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 324 GGGCCC GGGCC^C
Asp700I 1258 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 422 GGTACC G^GTACC
AspEI 814 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1186 CCTAGG C^CTAGG
BbvCI 1963 GCTGAGG CCTCAGC(-5/-2)
BclI 2166 TGATCA T^GATCA
BglI 317 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 864 AGATCT A^GATCT
BlnI 1186 CCTAGG C^CTAGG
BsaBI 1912 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1990 GACGTC G[GA]^CG[CT]C
BsaWI 1791 TCCGGA [AT]^CCGG[AT]
BseAI 1791 TCCGGA T^CCGGA
BspEI 1791 TCCGGA T^CCGGA
BspHI 1217 TCATGA T^CATGA
BsrFI 180 GCCGGC [GA]^CCGG[CT]
Cfr10I 180 GCCGGC [GA]^CCGG[CT]
DraII 323 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1441 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 814 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 323 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2089 GAATTC G^AATTC
FbaI 2166 TGATCA T^GATCA
HgaI 73 GCGTC GACGC(5/10)
Hin1I 1990 GACGTC G[GA]^CG[CT]C
HindIII 1516 AAGCTT A^AGCTT
KpnI 422 GGTACC GGTAC^C
KspI 109 CCGCGG CCGC^GG
MaeII 1991 ACGT A^CGT
MamI 1912 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 725 CAATTG C^AATTG
MroI 1791 TCCGGA T^CCGGA
MunI 725 CAATTG C^AATTG
NaeI 180 GCCGGC GCC^GGC
NgoMIV 180 GCCGGC G^CCGGC
PacI 1387 TTAATTAA TTAAT^TAA
PaeR7I 130 CTCGAG C^TCGAG
PstI 319 CTGCAG CTGCA^G
PvuII 1196 CAGCTG CAG^CTG
RcaI 1217 TCATGA T^CATGA
SacII 109 CCGCGG CCGC^GG
SbfI 318 CCTGCAGG CCTGCA^GG
ScaI 1816 AGTACT AGT^ACT
SfiI 316 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 318 CCTGCAGG CCTGCA^GG
SspI 899 AATATT AAT^ATT
XhoI 130 CTCGAG C^TCGAG
XmnI 1258 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 72 CGCG CG^CG
110 CGCG
AvaII 121 GGTCC G^G[AT]CC
547 GGTCC
BbvI 1675 GCAGC GCAGC(8/12)
197 GCTGC
BpmI 779 CTGGAG CTGGAG(16/14)
2135 CTGGAG
BsmAI 302 GAGAC GTCTC(1/5)
1439 GAGAC
BsrBI 94 CCGCTC CCGCTC(-3/-3)
133 GAGCGG
BstUI 72 CGCG CG^CG
110 CGCG
CfoI 220 GCGC GCG^C
1961 GCGC
EaeI 188 CGGCCA [CT]^GGCC[GA]
347 TGGCCG
EarI 1592 CTCTTC CTCTTC(1/4)
397 GAAGAG
HaeII 219 GGCGCT [GA]GCGC^[CT]
1960 GGCGCT
HhaI 220 GCGC GCG^C
1961 GCGC
HinP1I 220 GCGC G^CGC
1961 GCGC
HincII 1132 GTTGAC GT[CT]^[GA]AC
1297 GTTGAC
HindII 1132 GTTGAC GT[CT]^[GA]AC
1297 GTTGAC
Ksp632I 1592 CTCTTC CTCTTC(1/4)
397 GAAGAG
MslI 472 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1417 CATTTTGATG
MvnI 72 CGCG CG^CG
110 CGCG
NcoI 1956 CCATGG C^CATGG
2063 CCATGG
NspI 507 ACATGC [GA]CATG^[CT]
2035 ACATGC
SfaNI 733 GATGC GCATC(5/9)
1105 GCATC
SfcI 319 CTGCAG C^T[GA][CT]AG
851 CTGTAG
SmaI 141 CCCGGG CCC^GGG
201 CCCGGG
TseI 197 GCTGC G^C[AT]GC
1675 GCAGC
XmaCI 141 CCCGGG C^CCGGG
201 CCCGGG
XmaI 141 CCCGGG C^CCGGG
201 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaI
BanI BanII BbsI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SmlI StyI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XhoII