Restriction map for
pF1KB7582
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# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AcsI 547 GAATTC [GA]^AATT[CT]
AfaI 354 GTAC GT^AC
AlwNI 701 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 230 GGGCCC GGGCC^C
ApoI 547 GAATTC [GA]^AATT[CT]
AspHI 823 GAGCTC G[AT]GC[AT]^C
AspI 141 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 408 GGATCC G^GATCC
BpmI 803 CTCCAG CTGGAG(16/14)
BsiHKAI 823 GAGCTC G[AT]GC[AT]^C
BsmFI 338 GTCCC GGGAC(10/14)
EcoRI 547 GAATTC G^AATTC
MaeII 670 ACGT A^CGT
MaeIII 98 GTCAC ^GT[ACGT]AC
MseI 574 TTAA T^TAA
MspA1I 281 CAGCTG C[AC]G^C[GT]G
NdeI 190 CATATG CA^TATG
PflFI 141 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 460 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 336 GAGTC GAGTC(4/5)
PpuMI 15 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 281 CAGCTG CAG^CTG
RsaI 354 GTAC GT^AC
SacI 823 GAGCTC GAGCT^C
TfiI 387 GATTC G^A[AT]TC
Tru9I 574 TTAA T^TAA
Tsp45I 98 GTCAC ^GT[GC]AC
TspRI 167 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 141 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 460 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 16 GGACC G^G[AT]CC
625 GGTCC
BanII 230 GGGCCC G[GA]GC[CT]^C
823 GAGCTC
BmyI 230 GGGCCC G[AGT]GC[ACT]^C
823 GAGCTC
BsaJI 758 CCAAGG C^C[ACGT][ACGT]GG
788 CCTTGG
Bsp1286I 230 GGGCCC G[AGT]GC[ACT]^C
823 GAGCTC
BsrI 168 ACTGG ACTGG(1/-1)
748 ACTGG
BssKI 27 CCTGG ^CC[ACGT]GG
725 CCTGG
BstAPI 316 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
700 GCAGAAACTGC
BstNI 27 CCTGG CC^[AT]GG
725 CCTGG
BstYI 395 GGATCT [GA]^GATC[CT]
408 GGATCC
DpnII 396 GATC ^GATC
409 GATC
DraII 15 AGGACCC [GA]G^G[ACGT]CC[CT]
229 AGGGCCC
EarI 82 GAAGAG CTCTTC(1/4)
534 GAAGAG
EcoO109I 15 AGGACCC [GA]G^G[ACGT]CC[CT]
229 AGGGCCC
EcoRII 27 CCTGG ^CC[AT]GG
725 CCTGG
EcoT14I 758 CCAAGG C^C[AT][AT]GG
788 CCTTGG
HinfI 336 GAGTC G^A[ACGT]TC
387 GATTC
Ksp632I 82 GAAGAG CTCTTC(1/4)
534 GAAGAG
MboI 396 GATC ^GATC
409 GATC
MflI 395 GGATCT [GA]^GATC[CT]
408 GGATCC
MvaI 27 CCTGG CC^[AT]GG
725 CCTGG
NdeII 396 GATC ^GATC
409 GATC
NspI 540 GCATGT [GA]CATG^[CT]
696 ACATGC
Sau3AI 396 GATC ^GATC
409 GATC
ScrFI 27 CCTGG CC^[ACGT]GG
725 CCTGG
StyI 758 CCAAGG C^C[AT][AT]GG
788 CCTTGG
XhoII 395 GGATCT [GA]^GATC[CT]
408 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AlwI BbvI BseRI BsiYI
BslI BstF5I Cac8I Cfr13I DdeI
Fnu4HI FokI HaeIII ItaI MboII
MnlI MwoI NlaIII NlaIV PstI
Sau96I SfaNI SfcI TseI Tsp509I