Restriction map for
pF1KE6787
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII BalI BamHI BbeI
BcgI BcnI BglI BlpI Bpu1102I
BsaHI BsaI BsgI BsiEI BsmBI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FauI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 8 TCCGGA T^CCGGA
AciI 741 CCGC CCGC(-3/-1)
AflII 1776 CTTAAG C^TTAAG
Aor51HI 1155 AGCGCT AGC^GCT
AseI 1350 ATTAAT AT^TAAT
AsnI 1350 ATTAAT AT^TAAT
Asp700I 1942 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1771 GTGCTC G[AT]GC[AT]^C
AspI 1230 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2019 CCTAGG C^CTAGG
BanII 1137 GGGCTC G[GA]GC[CT]^C
BbrPI 1447 CACGTG CAC^GTG
BbsI 1797 GAAGAC GAAGAC(2/6)
BciVI 25 GTATCC GTATCC(6/5)
BfrI 1776 CTTAAG C^TTAAG
BglII 380 AGATCT A^GATCT
BlnI 2019 CCTAGG C^CTAGG
BpuAI 1797 GAAGAC GAAGAC(2/6)
BsaAI 1447 CACGTG [CT]AC^GT[GA]
BsaWI 8 TCCGGA [AT]^CCGG[AT]
BseAI 8 TCCGGA T^CCGGA
BsiHKAI 1771 GTGCTC G[AT]GC[AT]^C
BsiWI 727 CGTACG C^GTACG
BsmI 239 GAATGC GAATGC(1/-1)
BspEI 8 TCCGGA T^CCGGA
BsrFI 126 ACCGGC [GA]^CCGG[CT]
BstAPI 254 GCAATCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 157 GGTGACC G^GT[ACGT]ACC
BstPI 157 GGTGACC G^GT[ACGT]ACC
BstXI 907 CCAGTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1156 GCGC GCG^C
Cfr10I 126 ACCGGC [GA]^CCGG[CT]
EaeI 1220 TGGCCG [CT]^GGCC[GA]
Eco47III 1155 AGCGCT AGC^GCT
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
EcoRV 496 GATATC GAT^ATC
HaeII 1155 AGCGCT [GA]GCGC^[CT]
HgaI 1243 GACGC GACGC(5/10)
HhaI 1156 GCGC GCG^C
HinP1I 1156 GCGC G^CGC
MaeII 1448 ACGT A^CGT
MroI 8 TCCGGA T^CCGGA
PflFI 1230 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1447 CACGTG CAC^GTG
PmlI 1447 CACGTG CAC^GTG
PshBI 1350 ATTAAT AT^TAAT
PstI 562 CTGCAG CTGCA^G
SspI 2062 AATATT AAT^ATT
Tth111I 1230 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1942 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 1423 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1904 CAGTCACTG
AocI 447 CCTCAGG CC^T[ACGT]AGG
1264 CCTCAGG
BanI 33 GGTGCC G^G[CT][GA]CC
218 GGTGCC
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
1032 CCTCAGC
BclI 632 TGATCA T^GATCA
852 TGATCA
BsaBI 250 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1722 GATTGCTATC
BspHI 1625 TCATGA T^CATGA
1737 TCATGA
BspMI 134 ACCTGC ACCTGC(4/8)
925 ACCTGC
BstYI 380 AGATCT [GA]^GATC[CT]
1895 GGATCT
Bsu36I 447 CCTCAGG CC^T[ACGT]AGG
1264 CCTCAGG
Cac8I 1498 GCAGGC GC[ACGT]^[ACGT]GC
1646 GCCTGC
DraII 968 AGGGCCT [GA]G^G[ACGT]CC[CT]
1424 AGGGCCT
Eco81I 447 CCTCAGG CC^T[ACGT]AGG
1264 CCTCAGG
EcoNI 135 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
447 CCTCAGGCAGG
EcoO109I 968 AGGGCCT [GA]G^G[ACGT]CC[CT]
1424 AGGGCCT
FbaI 632 TGATCA T^GATCA
852 TGATCA
HapII 9 CCGG C^CGG
127 CCGG
HpaII 9 CCGG C^CGG
127 CCGG
MamI 250 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1722 GATTGCTATC
MflI 380 AGATCT [GA]^GATC[CT]
1895 GGATCT
MspI 9 CCGG C^CGG
127 CCGG
NcoI 881 CCATGG C^CATGG
1365 CCATGG
PflMI 540 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1297 CCAGAATTTGG
RcaI 1625 TCATGA T^CATGA
1737 TCATGA
ScaI 518 AGTACT AGT^ACT
787 AGTACT
SfaNI 274 GATGC GCATC(5/9)
1361 GCATC
SmlI 180 CTCAAG C^T[CT][GA]AG
1776 CTTAAG
Van91I 540 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1297 CCAGAATTTGG
XhoII 380 AGATCT [GA]^GATC[CT]
1895 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI