Restriction map for
pF1KE1911
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AviII
AvrII BalI BbrPI BbsI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EarI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI ScaI SfiI
SfuI SmaI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 442 GGTACC G^GTACC
AcyI 49 GGCGCC G[GA]^CG[CT]C
AhdI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 13 GTGCAC G^TGCAC
AlwNI 1123 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 235 GGGCCC GGGCC^C
ApaLI 13 GTGCAC G^TGCAC
Asp718I 442 GGTACC G^GTACC
AspEI 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 426 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 771 GGTCC G^G[AT]CC
BamHI 1031 GGATCC G^GATCC
BbeI 49 GGCGCC GGCGC^C
BbvCI 1318 GCTGAGG CCTCAGC(-5/-2)
BcgI 38 GCAACCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 68 TGATCA T^GATCA
BglI 237 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 248 AGATCT A^GATCT
BpmI 202 CTGGAG CTGGAG(16/14)
BsaAI 175 TACGTG [CT]AC^GT[GA]
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsiEI 256 CGGCCG CG[GA][CT]^CG
BsmAI 592 GAGAC GTCTC(1/5)
BspHI 488 TCATGA T^CATGA
BsrFI 163 ACCGGC [GA]^CCGG[CT]
BssSI 193 CTCGTG CACGAG(-5/-1)
Cfr10I 163 ACCGGC [GA]^CCGG[CT]
DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 256 CGGCCG C^GGCCG
Eam1105I 1249 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 256 CGGCCG C^GGCCG
Eco52I 256 CGGCCG C^GGCCG
FbaI 68 TGATCA T^GATCA
Hin1I 49 GGCGCC G[GA]^CG[CT]C
KasI 49 GGCGCC G^GCGCC
KpnI 442 GGTACC GGTAC^C
MseI 992 TTAA T^TAA
NarI 49 GGCGCC GG^CGCC
NspI 959 GCATGT [GA]CATG^[CT]
PflFI 426 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 488 TCATGA T^CATGA
SacI 862 GAGCTC GAGCT^C
SbfI 387 CCTGCAGG CCTGCA^GG
SfoI 49 GGCGCC GGC^GCC
SgrAI 162 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 1175 AGGCCT AGG^CCT
Tru9I 992 TTAA T^TAA
Tth111I 426 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 184 CGCG CG^CG
327 CGCG
AflIII 143 ACGTGT A^C[GA][CT]GT
494 ACACGT
BsgI 787 CTGCAC GTGCAG(16/14)
1183 CTGCAC
BstEII 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstUI 184 CGCG CG^CG
327 CGCG
DraII 234 GGGGCCC [GA]G^G[ACGT]CC[CT]
235 GGGCCCT
EcoO109I 234 GGGGCCC [GA]G^G[ACGT]CC[CT]
235 GGGCCCT
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
FauI 182 CCCGC CCCGC(4/6)
914 CCCGC
HaeII 49 GGCGCC [GA]GCGC^[CT]
846 GGCGCT
HinfI 220 GACTC G^A[ACGT]TC
340 GACTC
MboII 251 TCTTC GAAGA(8/7)
794 TCTTC
MspA1I 127 CAGCTG C[AC]G^C[GT]G
400 CAGCTG
MvnI 184 CGCG CG^CG
327 CGCG
NcoI 215 CCATGG C^CATGG
918 CCATGG
PflMI 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCACTCCCTGG
PleI 220 GACTC GAGTC(4/5)
340 GACTC
PstI 124 CTGCAG CTGCA^G
388 CTGCAG
PvuII 127 CAGCTG CAG^CTG
400 CAGCTG
SexAI 680 ACCTGGT A^CC[AT]GGT
1250 ACCTGGT
SfcI 124 CTGCAG C^T[GA][CT]AG
388 CTGCAG
TaqI 89 TCGA T^CGA
1307 TCGA
TthHB8I 89 TCGA T^CGA
1307 TCGA
Van91I 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCACTCCCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
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AciI AfaI AluI AlwI AspHI
AvaI BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI
XcmI XhoII