Restriction map for
pF1KE3319
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI BcgI BclI BfrI BglII
BsaI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI MfeI MluI
MseI MunI NaeI NcoI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 1209 GGTACC G^GTACC
AccIII 329 TCCGGA T^CCGGA
AflIII 386 ACACGT A^C[GA][CT]GT
AhdI 2638 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1209 GGTACC G^GTACC
AspEI 2638 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 746 TGCGCA TGC^GCA
AvrII 1609 CCTAGG C^CTAGG
BamHI 1826 GGATCC G^GATCC
BbrPI 387 CACGTG CAC^GTG
BciVI 787 GGATAC GTATCC(6/5)
BlnI 1609 CCTAGG C^CTAGG
BseAI 329 TCCGGA T^CCGGA
BspEI 329 TCCGGA T^CCGGA
BstXI 590 CCAAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2305 GACTGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2638 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1551 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 746 TGCGCA TGC^GCA
HgaI 2323 GACGC GACGC(5/10)
HincII 2314 GTCAAC GT[CT]^[GA]AC
HindII 2314 GTCAAC GT[CT]^[GA]AC
KpnI 1209 GGTACC GGTAC^C
KspI 16 CCGCGG CCGC^GG
MroI 329 TCCGGA T^CCGGA
NdeI 1600 CATATG CA^TATG
NotI 901 GCGGCCGC GC^GGCCGC
NspI 342 GCATGT [GA]CATG^[CT]
PflMI 2459 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 387 CACGTG CAC^GTG
PmlI 387 CACGTG CAC^GTG
PshAI 1068 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 16 CCGCGG CCGC^GG
SfiI 903 GGCCGCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1457 AGGCCT AGG^CCT
Tsp509I 1483 AATT ^AATT
Van91I 2459 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 275 CCTCAGG CC^T[ACGT]AGG
1323 CCTGAGG
AspHI 1404 GAGCAC G[AT]GC[AT]^C
1783 GTGCTC
BalI 128 TGGCCA TGG^CCA
911 TGGCCA
BbsI 1497 GAAGAC GAAGAC(2/6)
1963 GTCTTC
BpuAI 1497 GAAGAC GAAGAC(2/6)
1963 GTCTTC
BsaAI 387 CACGTG [CT]AC^GT[GA]
1990 TACGTG
BsaBI 480 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1944 GATGCAGATC
BsaWI 329 TCCGGA [AT]^CCGG[AT]
2141 ACCGGA
BsiEI 286 CGGCCG CG[GA][CT]^CG
902 CGGCCG
BsiHKAI 1404 GAGCAC G[AT]GC[AT]^C
1783 GTGCTC
BsmAI 1158 GAGAC GTCTC(1/5)
1576 GAGAC
BspHI 1373 TCATGA T^CATGA
1940 TCATGA
BssSI 1954 CACGAG CACGAG(-5/-1)
2026 CACGAG
BstEII 1236 GGTGACC G^GT[ACGT]ACC
1933 GGTGACC
BstPI 1236 GGTGACC G^GT[ACGT]ACC
1933 GGTGACC
BstYI 1826 GGATCC [GA]^GATC[CT]
1949 AGATCC
Bsu36I 275 CCTCAGG CC^T[ACGT]AGG
1323 CCTGAGG
DsaI 16 CCGCGG C^C[GA][CT]GG
289 CCGTGG
EagI 286 CGGCCG C^GGCCG
902 CGGCCG
EarI 1513 GAAGAG CTCTTC(1/4)
2293 GAAGAG
EclXI 286 CGGCCG C^GGCCG
902 CGGCCG
Eco52I 286 CGGCCG C^GGCCG
902 CGGCCG
Eco81I 275 CCTCAGG CC^T[ACGT]AGG
1323 CCTGAGG
EcoO65I 1236 GGTGACC G^GT[ACGT]ACC
1933 GGTGACC
Ksp632I 1513 GAAGAG CTCTTC(1/4)
2293 GAAGAG
MamI 480 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1944 GATGCAGATC
MflI 1826 GGATCC [GA]^GATC[CT]
1949 AGATCC
MluNI 128 TGGCCA TGG^CCA
911 TGGCCA
MscI 128 TGGCCA TGG^CCA
911 TGGCCA
MslI 955 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1954 CACGAGGATG
RcaI 1373 TCATGA T^CATGA
1940 TCATGA
SmlI 1162 CTTGAG C^T[CT][GA]AG
2359 CTTGAG
XcmI 179 CCAACCGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
980 CCAGCGAGGTGCTGG
XhoII 1826 GGATCC [GA]^GATC[CT]
1949 AGATCC
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I