Restriction map for
pF1KE6507
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BalI BamHI BbrPI BbvCI BciVI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BseRI BsiWI BsmBI
BsmI Bsp1407I BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DraI DraIII
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MluNI MscI
MslI MunI NcoI NdeI NheI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccIII 616 TCCGGA T^CCGGA
AcsI 228 GAATTT [GA]^AATT[CT]
AfaI 522 GTAC GT^AC
AflIII 353 ACACGT A^C[GA][CT]GT
AhdI 532 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 228 GAATTT [GA]^AATT[CT]
AspEI 532 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 565 GAGCTC G[AT]GC[AT]^C
AviII 125 TGCGCA TGC^GCA
BanI 14 GGCGCC G^G[CT][GA]CC
BanII 565 GAGCTC G[GA]GC[CT]^C
BbeI 14 GGCGCC GGCGC^C
BbvI 552 GCTGC GCAGC(8/12)
BclI 431 TGATCA T^GATCA
BglII 414 AGATCT A^GATCT
BmyI 565 GAGCTC G[AGT]GC[ACT]^C
BsaI 274 GAGACC GGTCTC(1/5)
BsaWI 616 TCCGGA [AT]^CCGG[AT]
BseAI 616 TCCGGA T^CCGGA
BsgI 147 CTGCAC GTGCAG(16/14)
BsiHKAI 565 GAGCTC G[AT]GC[AT]^C
Bsp1286I 565 GAGCTC G[AGT]GC[ACT]^C
BspDI 136 ATCGAT AT^CGAT
BspEI 616 TCCGGA T^CCGGA
BspMI 145 ACCTGC ACCTGC(4/8)
BstBI 168 TTCGAA TT^CGAA
BstF5I 476 CATCC GGATG(2/0)
BstYI 414 AGATCT [GA]^GATC[CT]
ClaI 136 ATCGAT AT^CGAT
DdeI 488 CTAAG C^T[ACGT]AG
DraII 77 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 364 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 532 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 77 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 605 CCTTGG C^C[AT][AT]GG
FbaI 431 TGATCA T^GATCA
FokI 476 CATCC GGATG(9/13)
FspI 125 TGCGCA TGC^GCA
HaeII 14 GGCGCC [GA]GCGC^[CT]
KasI 14 GGCGCC G^GCGCC
KspI 82 CCGCGG CCGC^GG
MaeIII 42 GTCAC ^GT[ACGT]AC
MflI 414 AGATCT [GA]^GATC[CT]
MroI 616 TCCGGA T^CCGGA
MspA1I 82 CCGCGG C[AC]G^C[GT]G
NarI 14 GGCGCC GG^CGCC
NlaIII 547 CATG CATG^
NotI 110 GCGGCCGC GC^GGCCGC
NspV 168 TTCGAA TT^CGAA
PflMI 475 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 77 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 522 GTAC GT^AC
SacI 565 GAGCTC GAGCT^C
SacII 82 CCGCGG CCGC^GG
SfiI 112 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 14 GGCGCC GGC^GCC
SfuI 168 TTCGAA TT^CGAA
SmaI 74 CCCGGG CCC^GGG
StyI 605 CCTTGG C^C[AT][AT]GG
TseI 552 GCTGC G^C[AT]GC
Tsp45I 42 GTCAC ^GT[GC]AC
Tsp509I 229 AATT ^AATT
TspRI 480 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 475 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 267 TCTAGA T^CTAGA
XhoII 414 AGATCT [GA]^GATC[CT]
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AluI 551 AGCT AG^CT
566 AGCT
AvaI 10 CTCGGG C^[CT]CG[GA]G
74 CCCGGG
AvaII 78 GGTCC G^G[AT]CC
484 GGACC
BbsI 331 GAAGAC GAAGAC(2/6)
600 GTCTTC
BcgI 132 CGACATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
128 GCACCGACATCG
BglI 113 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
254 GCCACCCAGGC
BpuAI 331 GAAGAC GAAGAC(2/6)
600 GTCTTC
BsiEI 85 CGGCCG CG[GA][CT]^CG
111 CGGCCG
BsmAI 274 GAGAC GTCTC(1/5)
373 GTCTC
BsmFI 79 GTCCC GGGAC(10/14)
540 GTCCC
BsoBI 10 CTCGGG C^[CT]CG[GA]G
74 CCCGGG
BsrFI 28 GCCGGC [GA]^CCGG[CT]
87 GCCGGC
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
87 GCCGGC
DpnII 415 GATC ^GATC
432 GATC
DsaI 82 CCGCGG C^C[GA][CT]GG
117 CCGTGG
EagI 85 CGGCCG C^GGCCG
111 CGGCCG
EclXI 85 CGGCCG C^GGCCG
111 CGGCCG
Eco52I 85 CGGCCG C^GGCCG
111 CGGCCG
FauI 81 CCCGC CCCGC(4/6)
155 CCCGC
HgaI 444 GACGC GACGC(5/10)
40 GCGTC
HinfI 6 GACTC G^A[ACGT]TC
310 GACTC
HphI 43 TCACC GGTGA(8/7)
71 TCACC
MaeII 177 ACGT A^CGT
355 ACGT
MboI 415 GATC ^GATC
432 GATC
MseI 394 TTAA T^TAA
580 TTAA
NaeI 28 GCCGGC GCC^GGC
87 GCCGGC
NdeII 415 GATC ^GATC
432 GATC
NgoMIV 28 GCCGGC G^CCGGC
87 GCCGGC
PleI 6 GACTC GAGTC(4/5)
310 GACTC
Sau3AI 415 GATC ^GATC
432 GATC
SfaNI 139 GATGC GCATC(5/9)
625 GATGC
TaqI 137 TCGA T^CGA
169 TCGA
Tru9I 394 TTAA T^TAA
580 TTAA
TthHB8I 137 TCGA T^CGA
169 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AcyI BcnI BfaI
BsaHI BsaJI BsiYI BslI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EaeI EcoRII Fnu4HI HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI MaeI MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI