Restriction map for
pF1KE2328
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AocI
ApaI ApoI AscI Asp718I AviII
AvrII BalI BamHI BanI BanII
BbeI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiWI BsmI BspDI BspEI BsrDI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Bsu36I ClaI CpoI
DraI DraIII DrdI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 133 GTATAC GT^[AC][GT]AC
AflIII 581 ACATGT A^C[GA][CT]GT
AhdI 19 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 993 GTGCAC G^TGCAC
Aor51HI 68 AGCGCT AGC^GCT
ApaLI 993 GTGCAC G^TGCAC
AseI 846 ATTAAT AT^TAAT
AsnI 846 ATTAAT AT^TAAT
AspEI 19 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 733 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 769 CACGTG CAC^GTG
BbvCI 108 CCTCAGC CCTCAGC(-5/-2)
BpmI 508 CTCCAG CTGGAG(16/14)
BsgI 766 CTGCAC GTGCAG(16/14)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsmAI 601 GAGAC GTCTC(1/5)
BsmBI 601 GAGACG CGTCTC(1/5)
Bsp1407I 532 TGTACA T^GTACA
BspHI 434 TCATGA T^CATGA
BspLU11I 581 ACATGT A^CATGT
BsrFI 785 ACCGGC [GA]^CCGG[CT]
BsrGI 532 TGTACA T^GTACA
Bst1107I 133 GTATAC GTA^TAC
BstAPI 113 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 133 GTATAC GTA^TAC
Cac8I 651 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 785 ACCGGC [GA]^CCGG[CT]
DraII 731 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 282 CCATGG C^C[GA][CT]GG
EaeI 53 CGGCCG [CT]^GGCC[GA]
EagI 53 CGGCCG C^GGCCG
Eam1105I 19 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 53 CGGCCG C^GGCCG
Eco47III 68 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
EcoO109I 731 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 729 GCGGG CCCGC(4/6)
HindIII 305 AAGCTT A^AGCTT
NcoI 282 CCATGG C^CATGG
PflFI 733 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 548 GAGTC GAGTC(4/5)
PmaCI 769 CACGTG CAC^GTG
PmlI 769 CACGTG CAC^GTG
PpuMI 731 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 846 ATTAAT AT^TAAT
RcaI 434 TCATGA T^CATGA
SapI 84 GAAGAGC GCTCTTC(1/4)
ScaI 268 AGTACT AGT^ACT
SexAI 734 ACCTGGT A^CC[AT]GGT
SfaNI 416 GCATC GCATC(5/9)
SmaI 31 CCCGGG CCC^GGG
SspBI 532 TGTACA T^GTACA
SspI 439 AATATT AAT^ATT
Tsp509I 849 AATT ^AATT
Tth111I 733 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 31 CCCGGG C^CCGGG
XmaI 31 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwI 834 GATCC GGATC(4/5)
248 GGATC
Asp700I 66 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
147 GAATCTTTTC
AspHI 772 GTGCTC G[AT]GC[AT]^C
993 GTGCAC
AvaI 10 CTCGGG C^[CT]CG[GA]G
31 CCCGGG
BcnI 31 CCCGG CC^[GC]GG
32 CCGGG
BlpI 717 GCTGAGC GC^T[ACGT]AGC
774 GCTCAGC
Bpu1102I 717 GCTGAGC GC^T[ACGT]AGC
774 GCTCAGC
BsaAI 361 TACGTG [CT]AC^GT[GA]
769 CACGTG
BsiHKAI 772 GTGCTC G[AT]GC[AT]^C
993 GTGCAC
BsmFI 731 GGGAC GGGAC(10/14)
27 GTCCC
BsoBI 10 CTCGGG C^[CT]CG[GA]G
31 CCCGGG
BspMI 680 ACCTGC ACCTGC(4/8)
764 ACCTGC
BsrBI 87 GAGCGG CCGCTC(-3/-3)
826 GAGCGG
BstYI 248 GGATCT [GA]^GATC[CT]
833 AGATCC
CelII 717 GCTGAGC GC^T[ACGT]AGC
774 GCTCAGC
CfoI 69 GCGC GCG^C
279 GCGC
EarI 84 GAAGAG CTCTTC(1/4)
186 GAAGAG
EcoNI 9 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
744 CCTGGGCCAGG
EcoT14I 282 CCATGG C^C[AT][AT]GG
913 CCAAGG
HaeII 68 AGCGCT [GA]GCGC^[CT]
278 AGCGCC
HhaI 69 GCGC GCG^C
279 GCGC
HinP1I 69 GCGC G^CGC
279 GCGC
Ksp632I 84 GAAGAG CTCTTC(1/4)
186 GAAGAG
MaeII 362 ACGT A^CGT
770 ACGT
MflI 248 GGATCT [GA]^GATC[CT]
833 AGATCC
MspA1I 625 CAGCTG C[AC]G^C[GT]G
868 CAGCTG
NciI 31 CCCGG CC^[GC]GG
32 CCGGG
NlaIV 26 GGTCCC GG[ACGT]^[ACGT]CC
731 GGGACC
NspI 200 ACATGC [GA]CATG^[CT]
581 ACATGT
PvuII 625 CAGCTG CAG^CTG
868 CAGCTG
StuI 649 AGGCCT AGG^CCT
663 AGGCCT
StyI 282 CCATGG C^C[AT][AT]GG
913 CCAAGG
Tsp45I 393 GTCAC ^GT[GC]AC
959 GTCAC
XhoII 248 GGATCT [GA]^GATC[CT]
833 AGATCC
XmnI 66 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
147 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbsI BbvI BmyI BpuAI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI TspRI