Restriction map for
pF1KE2587
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BcnI BfrI BglII BlnI BsaAI
BsaHI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MluI MspA1I
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 619 TCCGGA T^CCGGA
AhdI 148 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 220 GTGCAC G^TGCAC
ApaLI 220 GTGCAC G^TGCAC
AspEI 148 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 479 TGGCCA TGG^CCA
BamHI 431 GGATCC G^GATCC
BanII 893 GAGCTC G[GA]GC[CT]^C
BbvCI 1044 CCTCAGC CCTCAGC(-5/-2)
BglI 964 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 705 GCTGAGC GC^T[ACGT]AGC
BpmI 1754 CTGGAG CTGGAG(16/14)
Bpu1102I 705 GCTGAGC GC^T[ACGT]AGC
BsaBI 903 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 245 GGTCTC GGTCTC(1/5)
BsaWI 619 TCCGGA [AT]^CCGG[AT]
BseAI 619 TCCGGA T^CCGGA
BspDI 1306 ATCGAT AT^CGAT
BspEI 619 TCCGGA T^CCGGA
BspMI 1500 GCAGGT ACCTGC(4/8)
BstAPI 805 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 183 GGTGACC G^GT[ACGT]ACC
BstPI 183 GGTGACC G^GT[ACGT]ACC
CelII 705 GCTGAGC GC^T[ACGT]AGC
CfoI 539 GCGC GCG^C
ClaI 1306 ATCGAT AT^CGAT
DraII 1191 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 223 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 479 TGGCCA [CT]^GGCC[GA]
Eam1105I 148 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1494 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1191 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 183 GGTGACC G^GT[ACGT]ACC
EcoRI 878 GAATTC G^AATTC
EcoRV 1615 GATATC GAT^ATC
FauI 1527 CCCGC CCCGC(4/6)
HapII 620 CCGG C^CGG
HgaI 1409 GACGC GACGC(5/10)
HhaI 539 GCGC GCG^C
HinP1I 539 GCGC G^CGC
HpaII 620 CCGG C^CGG
MamI 903 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1010 CAATTG C^AATTG
MluNI 479 TGGCCA TGG^CCA
MroI 619 TCCGGA T^CCGGA
MscI 479 TGGCCA TGG^CCA
MspI 620 CCGG C^CGG
MunI 1010 CAATTG C^AATTG
PpuMI 1191 AGGACCT [GA]G^G[AT]CC[CT]
SacI 893 GAGCTC GAGCT^C
SmlI 980 CTCAAG C^T[CT][GA]AG
SphI 29 GCATGC GCATG^C
TaqI 1307 TCGA T^CGA
TthHB8I 1307 TCGA T^CGA
XcmI 272 CCATATTTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 1528 CCGC CCGC(-3/-1)
919 GCGG
AlwNI 146 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
524 CAGAGCCTG
AspHI 220 GTGCAC G[AT]GC[AT]^C
893 GAGCTC
BanI 111 GGCACC G^G[CT][GA]CC
304 GGCACC
BclI 533 TGATCA T^GATCA
923 TGATCA
BfaI 890 CTAG C^TAG
1867 CTAG
BsiHKAI 220 GTGCAC G[AT]GC[AT]^C
893 GAGCTC
BsmAI 246 GTCTC GTCTC(1/5)
1759 GTCTC
BsmI 1256 GAATGC GAATGC(1/-1)
1542 GAATGC
Bsp1407I 1115 TGTACA T^GTACA
1825 TGTACA
BsrGI 1115 TGTACA T^GTACA
1825 TGTACA
BssKI 1023 CCAGG ^CC[ACGT]GG
1719 CCTGG
BstNI 1023 CCAGG CC^[AT]GG
1719 CCTGG
BstYI 431 GGATCC [GA]^GATC[CT]
985 GGATCT
EcoRII 1023 CCAGG ^CC[AT]GG
1719 CCTGG
EcoT22I 46 ATGCAT ATGCA^T
647 ATGCAT
FbaI 533 TGATCA T^GATCA
923 TGATCA
HindIII 932 AAGCTT A^AGCTT
1679 AAGCTT
MaeI 890 CTAG C^TAG
1867 CTAG
MaeII 437 ACGT A^CGT
1852 ACGT
MflI 431 GGATCC [GA]^GATC[CT]
985 GGATCT
MvaI 1023 CCAGG CC^[AT]GG
1719 CCTGG
NcoI 1038 CCATGG C^CATGG
1094 CCATGG
NsiI 46 ATGCAT ATGCA^T
647 ATGCAT
NspI 29 GCATGC [GA]CATG^[CT]
365 ACATGC
PflMI 189 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
226 CCAGTGTTTGG
PleI 1870 GACTC GAGTC(4/5)
1757 GAGTC
PstI 443 CTGCAG CTGCA^G
1495 CTGCAG
ScrFI 1023 CCAGG CC^[ACGT]GG
1719 CCTGG
SfaNI 45 GATGC GCATC(5/9)
805 GCATC
SspBI 1115 TGTACA T^GTACA
1825 TGTACA
SspI 1004 AATATT AAT^ATT
1286 AATATT
Van91I 189 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
226 CCAGTGTTTGG
XhoII 431 GGATCC [GA]^GATC[CT]
985 GGATCT
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbsI BbvI BmyI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrI BstF5I BstXI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MwoI NdeI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI