Restriction map for
pF1KE3103
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI BalI BamHI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI HincII
HindII HindIII HpaI KpnI MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NdeI NdeII NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
SalI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 136 GGCGCC G[GA]^CG[CT]C
AscI 40 GGCGCGCC GG^CGCGCC
AspHI 238 GAGCTC G[AT]GC[AT]^C
AvrII 123 CCTAGG C^CTAGG
BanI 136 GGCGCC G^G[CT][GA]CC
BbeI 136 GGCGCC GGCGC^C
BcgI 599 GCAACAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 124 CTAG C^TAG
BlnI 123 CCTAGG C^CTAGG
BsaHI 136 GGCGCC G[GA]^CG[CT]C
BsgI 697 CTGCAC GTGCAG(16/14)
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
BspMI 411 GCAGGT ACCTGC(4/8)
BsrBI 341 CCGCTC CCGCTC(-3/-3)
BsrI 371 ACTGG ACTGG(1/-1)
CpoI 560 CGGACCG CG^G[AT]CCG
DdeI 683 CTGAG C^T[ACGT]AG
FseI 732 GGCCGGCC GGCCGG^CC
HgaI 385 GACGC GACGC(5/10)
Hin1I 136 GGCGCC G[GA]^CG[CT]C
HinfI 196 GACTC G^A[ACGT]TC
HphI 438 GGTGA GGTGA(8/7)
KasI 136 GGCGCC G^GCGCC
MaeI 124 CTAG C^TAG
MaeII 475 ACGT A^CGT
MslI 606 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 136 GGCGCC GG^CGCC
NcoI 200 CCATGG C^CATGG
NotI 448 GCGGCCGC GC^GGCCGC
PflMI 702 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 196 GACTC GAGTC(4/5)
PpuMI 725 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 757 CAGCTG CAG^CTG
RsrII 560 CGGACCG CG^G[AT]CCG
SacI 238 GAGCTC GAGCT^C
SfaNI 654 GATGC GCATC(5/9)
SfoI 136 GGCGCC GGC^GCC
SmlI 355 CTCAAG C^T[CT][GA]AG
Van91I 702 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 156 GTAC GT^AC
579 GTAC
AvaII 561 GGACC G^G[AT]CC
726 GGACC
AviII 424 TGCGCA TGC^GCA
614 TGCGCA
BpmI 220 CTGGAG CTGGAG(16/14)
292 CTGGAG
BsiEI 449 CGGCCG CG[GA][CT]^CG
782 CGGCCG
BsmFI 725 GGGAC GGGAC(10/14)
790 GGGAC
EagI 449 CGGCCG C^GGCCG
782 CGGCCG
EclXI 449 CGGCCG C^GGCCG
782 CGGCCG
Eco52I 449 CGGCCG C^GGCCG
782 CGGCCG
FspI 424 TGCGCA TGC^GCA
614 TGCGCA
KspI 102 CCGCGG CCGC^GG
564 CCGCGG
NlaIII 153 CATG CATG^
201 CATG
RsaI 156 GTAC GT^AC
579 GTAC
SacII 102 CCGCGG CCGC^GG
564 CCGCGG
SmaI 330 CCCGGG CCC^GGG
530 CCCGGG
TaqI 176 TCGA T^CGA
194 TCGA
TthHB8I 176 TCGA T^CGA
194 TCGA
XmaCI 330 CCCGGG C^CCGGG
530 CCCGGG
XmaI 330 CCCGGG C^CCGGG
530 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
BanII BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIV SapI Sau96I ScrFI
StyI TseI