Restriction map for
pF1KE1993
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I AlwNI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BalI
BbrPI BciVI BfaI BfrI BglII
BlnI BsaAI BsiWI BsmI BspDI
BspHI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
EarI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MfeI MluI MluNI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuII RcaI RsrII SacI SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1318 CGCG CG^CG
AcsI 483 AAATTC [GA]^AATT[CT]
AflIII 666 ACATGT A^C[GA][CT]GT
AhdI 382 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 682 CCTGAGG CC^T[ACGT]AGG
Aor51HI 459 AGCGCT AGC^GCT
ApaI 654 GGGCCC GGGCC^C
ApoI 483 AAATTC [GA]^AATT[CT]
Asp700I 1413 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 382 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1628 GTGCTC G[AT]GC[AT]^C
AspI 1222 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 781 GCTGAGG CCTCAGC(-5/-2)
BcgI 606 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 189 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 971 GAGACC GGTCTC(1/5)
BsgI 1600 CTGCAC GTGCAG(16/14)
BsiEI 42 CGATCG CG[GA][CT]^CG
BsiHKAI 1628 GTGCTC G[AT]GC[AT]^C
BsmBI 1562 CGTCTC CGTCTC(1/5)
Bsp1407I 497 TGTACA T^GTACA
BspLU11I 666 ACATGT A^CATGT
BspMI 1598 ACCTGC ACCTGC(4/8)
BsrBI 1078 GAGCGG CCGCTC(-3/-3)
BsrGI 497 TGTACA T^GTACA
BstUI 1318 CGCG CG^CG
Bsu36I 682 CCTGAGG CC^T[ACGT]AGG
DrdI 973 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1317 CCGCGG C^C[GA][CT]GG
Eam1105I 382 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 459 AGCGCT AGC^GCT
Eco81I 682 CCTGAGG CC^T[ACGT]AGG
EcoNI 1186 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 1317 CCGCGG CCGC^GG
MamI 189 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1318 CGCG CG^CG
PaeR7I 1285 CTCGAG C^TCGAG
PflFI 1222 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1134 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1235 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 42 CGATCG CGAT^CG
SacII 1317 CCGCGG CCGC^GG
ScaI 549 AGTACT AGT^ACT
SexAI 1223 ACCTGGT A^CC[AT]GGT
SfcI 1025 CTGTAG C^T[GA][CT]AG
SspBI 497 TGTACA T^GTACA
SspI 22 AATATT AAT^ATT
Tth111I 1222 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1134 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1285 CTCGAG C^TCGAG
XmnI 1413 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 1053 TCCGGA T^CCGGA
1592 TCCGGA
AvaI 658 CCCGAG C^[CT]CG[GA]G
1285 CTCGAG
AvaII 1236 GGACC G^G[AT]CC
1501 GGACC
BamHI 632 GGATCC G^GATCC
1210 GGATCC
BbeI 574 GGCGCC GGCGC^C
1677 GGCGCC
BglI 1015 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1546 GCCTCCTCGGC
BlpI 672 GCTGAGC GC^T[ACGT]AGC
1465 GCTGAGC
Bpu1102I 672 GCTGAGC GC^T[ACGT]AGC
1465 GCTGAGC
BsaWI 1053 TCCGGA [AT]^CCGG[AT]
1592 TCCGGA
BseAI 1053 TCCGGA T^CCGGA
1592 TCCGGA
BsmAI 971 GAGAC GTCTC(1/5)
1563 GTCTC
BsoBI 658 CCCGAG C^[CT]CG[GA]G
1285 CTCGAG
BspEI 1053 TCCGGA T^CCGGA
1592 TCCGGA
BstAPI 386 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1307 GCATGGAGTGC
CelII 672 GCTGAGC GC^T[ACGT]AGC
1465 GCTGAGC
EaeI 1553 CGGCCA [CT]^GGCC[GA]
1689 TGGCCG
FauI 744 CCCGC CCCGC(4/6)
1652 CCCGC
HgaI 1397 GACGC GACGC(5/10)
1524 GACGC
KasI 574 GGCGCC G^GCGCC
1677 GGCGCC
MroI 1053 TCCGGA T^CCGGA
1592 TCCGGA
MslI 756 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CATTGCTGTG
MspA1I 745 CCGCTG C[AC]G^C[GT]G
1317 CCGCGG
NarI 574 GGCGCC GG^CGCC
1677 GGCGCC
SfoI 574 GGCGCC GGC^GCC
1677 GGCGCC
SmlI 918 CTTGAG C^T[CT][GA]AG
1285 CTCGAG
XcmI 278 CCAAGGCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
591 CCACGCAGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AlwI BanI BanII BbsI BbvI
BclI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrDI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII