Restriction map for
pF1KE2690
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CpoI DraI DrdI EarI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tsp509I Van91I
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 1537 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 11 CCTCAGG CC^T[ACGT]AGG
AspEI 1537 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1396 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 894 CCTAGG C^CTAGG
BglI 1051 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 894 CCTAGG C^CTAGG
BlpI 813 GCTGAGC GC^T[ACGT]AGC
BpmI 1443 CTCCAG CTGGAG(16/14)
Bpu1102I 813 GCTGAGC GC^T[ACGT]AGC
BsiEI 140 CGGCCG CG[GA][CT]^CG
Bsp1407I 998 TGTACA T^GTACA
BspDI 748 ATCGAT AT^CGAT
BsrBI 294 CCGCTC CCGCTC(-3/-3)
BsrDI 620 GCAATG GCAATG(2/0)
BsrGI 998 TGTACA T^GTACA
BssSI 1086 CTCGTG CACGAG(-5/-1)
Bsu36I 11 CCTCAGG CC^T[ACGT]AGG
CelII 813 GCTGAGC GC^T[ACGT]AGC
ClaI 748 ATCGAT AT^CGAT
DraIII 267 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 140 CGGCCG C^GGCCG
Eam1105I 1537 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 140 CGGCCG C^GGCCG
Eco52I 140 CGGCCG C^GGCCG
Eco81I 11 CCTCAGG CC^T[ACGT]AGG
FseI 388 GGCCGGCC GGCCGG^CC
HaeII 574 GGCGCT [GA]GCGC^[CT]
MseI 566 TTAA T^TAA
NaeI 389 GCCGGC GCC^GGC
NcoI 1139 CCATGG C^CATGG
NgoMIV 389 GCCGGC G^CCGGC
PaeR7I 1360 CTCGAG C^TCGAG
PflFI 1396 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1400 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 1132 GAGCTC GAGCT^C
SexAI 173 ACCAGGT A^CC[AT]GGT
SfcI 198 CTACAG C^T[GA][CT]AG
SmaI 774 CCCGGG CCC^GGG
SmlI 1360 CTCGAG C^T[CT][GA]AG
SspBI 998 TGTACA T^GTACA
Tru9I 566 TTAA T^TAA
Tth111I 1396 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1533 TCTAGA T^CTAGA
XcmI 425 CCACCTTTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1360 CTCGAG C^TCGAG
XmaCI 774 CCCGGG C^CCGGG
XmaI 774 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 999 GTAC GT^AC
1038 GTAC
AlwNI 63 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
808 CAGCAGCTG
AspHI 361 GAGCAC G[AT]GC[AT]^C
1132 GAGCTC
AvaII 147 GGTCC G^G[AT]CC
1401 GGTCC
BalI 158 TGGCCA TGG^CCA
501 TGGCCA
BbsI 94 GTCTTC GAAGAC(2/6)
1608 GTCTTC
BpuAI 94 GTCTTC GAAGAC(2/6)
1608 GTCTTC
BsaAI 967 TACGTG [CT]AC^GT[GA]
1594 CACGTA
BsaI 688 GAGACC GGTCTC(1/5)
1261 GAGACC
BsiHKAI 361 GAGCAC G[AT]GC[AT]^C
1132 GAGCTC
BsrFI 245 ACCGGC [GA]^CCGG[CT]
389 GCCGGC
BssHII 378 GCGCGC G^CGCGC
1168 GCGCGC
BstXI 504 CCAACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
826 CCACCGCCCTGG
Cfr10I 245 ACCGGC [GA]^CCGG[CT]
389 GCCGGC
DraII 1247 AGGCCCT [GA]G^G[ACGT]CC[CT]
1400 AGGTCCT
EcoNI 168 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1377 CCTCTGTGAGG
EcoO109I 1247 AGGCCCT [GA]G^G[ACGT]CC[CT]
1400 AGGTCCT
HinfI 1084 GACTC G^A[ACGT]TC
1489 GACTC
MluNI 158 TGGCCA TGG^CCA
501 TGGCCA
MscI 158 TGGCCA TGG^CCA
501 TGGCCA
MslI 48 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
540 CACCCGGATG
PleI 1084 GACTC GAGTC(4/5)
1489 GACTC
PvuII 754 CAGCTG CAG^CTG
811 CAGCTG
RsaI 999 GTAC GT^AC
1038 GTAC
StuI 1202 AGGCCT AGG^CCT
1280 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
BamHI BanII BbvI BcnI BfaI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII