Restriction map for
pF1KE2672
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmI BspEI
BspLU11I BssHII Bst1107I BstBI BstUI
BstXI BstZ17I CelII CpoI DraI
DrdI Eam1105I EcoNI EcoRI EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI KspI MamI MluI
MroI MseI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1473 GGTACC G^GTACC
AcyI 1050 GACGCC G[GA]^CG[CT]C
Alw44I 334 GTGCAC G^TGCAC
Aor51HI 363 AGCGCT AGC^GCT
ApaLI 334 GTGCAC G^TGCAC
Asp700I 2206 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1473 GGTACC G^GTACC
BalI 1913 TGGCCA TGG^CCA
BamHI 2048 GGATCC G^GATCC
BbsI 2020 GAAGAC GAAGAC(2/6)
BglI 532 GCCCGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2031 AGATCT A^GATCT
BpuAI 2020 GAAGAC GAAGAC(2/6)
BsaHI 1050 GACGCC G[GA]^CG[CT]C
BsiEI 1096 CGGCCG CG[GA][CT]^CG
BspDI 1741 ATCGAT AT^CGAT
BspHI 1559 TCATGA T^CATGA
BsrDI 1842 CATTGC GCAATG(2/0)
BstEII 1431 GGTGACC G^GT[ACGT]ACC
BstPI 1431 GGTGACC G^GT[ACGT]ACC
CfoI 364 GCGC GCG^C
ClaI 1741 ATCGAT AT^CGAT
EagI 1096 CGGCCG C^GGCCG
EarI 243 GAAGAG CTCTTC(1/4)
EclXI 1096 CGGCCG C^GGCCG
Eco47III 363 AGCGCT AGC^GCT
Eco52I 1096 CGGCCG C^GGCCG
EcoO65I 1431 GGTGACC G^GT[ACGT]ACC
EcoRV 1267 GATATC GAT^ATC
FseI 26 GGCCGGCC GGCCGG^CC
HaeII 363 AGCGCT [GA]GCGC^[CT]
HgaI 1050 GACGC GACGC(5/10)
HhaI 364 GCGC GCG^C
Hin1I 1050 GACGCC G[GA]^CG[CT]C
HinP1I 364 GCGC G^CGC
KpnI 1473 GGTACC GGTAC^C
Ksp632I 243 GAAGAG CTCTTC(1/4)
MfeI 2199 CAATTG C^AATTG
MluNI 1913 TGGCCA TGG^CCA
MscI 1913 TGGCCA TGG^CCA
MunI 2199 CAATTG C^AATTG
NspI 1109 GCATGC [GA]CATG^[CT]
PshAI 194 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1559 TCATGA T^CATGA
SacI 457 GAGCTC GAGCT^C
SexAI 512 ACCTGGT A^CC[AT]GGT
SgrAI 1052 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1109 GCATGC GCATG^C
StuI 2066 AGGCCT AGG^CCT
XmnI 2206 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BanI 1117 GGTGCC G^G[CT][GA]CC
1473 GGTACC
BsgI 476 CTGCAC GTGCAG(16/14)
481 CTGCAC
BsmBI 17 GAGACG CGTCTC(1/5)
192 GAGACG
BsrBI 1882 GAGCGG CCGCTC(-3/-3)
1933 GAGCGG
DraIII 915 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1149 CACCATGTG
EaeI 1096 CGGCCG [CT]^GGCC[GA]
1913 TGGCCA
NaeI 27 GCCGGC GCC^GGC
105 GCCGGC
NgoMIV 27 GCCGGC G^CCGGC
105 GCCGGC
PflMI 1035 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1905 CCAGATTGTGG
SmaI 1205 CCCGGG CCC^GGG
2078 CCCGGG
Tsp509I 153 AATT ^AATT
2200 AATT
Van91I 1035 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1905 CCAGATTGTGG
XmaCI 1205 CCCGGG C^CCGGG
2078 CCCGGG
XmaI 1205 CCCGGG C^CCGGG
2078 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DsaI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XcmI XhoII