Restriction map for
pF1KE2434
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BmyI BpmI BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeI
MfeI MflI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 173 GTCTAC GT^[AC][GT]AC
AocI 675 CCTGAGG CC^T[ACGT]AGG
Asp700I 511 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 680 GGTCC G^G[AT]CC
BbsI 22 GTCTTC GAAGAC(2/6)
BbvCI 351 GCTGAGG CCTCAGC(-5/-2)
BclI 32 TGATCA T^GATCA
BlpI 831 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 831 GCTGAGC GC^T[ACGT]AGC
BpuAI 22 GTCTTC GAAGAC(2/6)
BsaI 318 GGTCTC GGTCTC(1/5)
BsgI 67 GTGCAG GTGCAG(16/14)
BsiYI 570 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 570 CCTGTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 319 GTCTC GTCTC(1/5)
BsmFI 681 GTCCC GGGAC(10/14)
BsmI 813 GCATTC GAATGC(1/-1)
BspHI 29 TCATGA T^CATGA
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
Cac8I 290 GCTTGC GC[ACGT]^[ACGT]GC
CelII 831 GCTGAGC GC^T[ACGT]AGC
CfoI 661 GCGC GCG^C
Cfr13I 680 GGTCC G^G[ACGT]CC
DpnII 33 GATC ^GATC
DraII 679 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 467 CCATGG C^C[GA][CT]GG
EarI 321 CTCTTC CTCTTC(1/4)
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
EcoO109I 679 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 467 CCATGG C^C[AT][AT]GG
EcoT22I 811 ATGCAT ATGCA^T
FbaI 32 TGATCA T^GATCA
HaeIII 579 GGCC GG^CC
HhaI 661 GCGC GCG^C
HinP1I 661 GCGC G^CGC
HindIII 785 AAGCTT A^AGCTT
Ksp632I 321 CTCTTC CTCTTC(1/4)
MaeII 844 ACGT A^CGT
MboI 33 GATC ^GATC
NcoI 467 CCATGG C^CATGG
NdeII 33 GATC ^GATC
NlaIV 680 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 811 ATGCAT ATGCA^T
PleI 49 GAGTC GAGTC(4/5)
PpuMI 679 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 29 TCATGA T^CATGA
Sau3AI 33 GATC ^GATC
Sau96I 680 GGTCC G^G[ACGT]CC
SfcI 175 CTACAG C^T[GA][CT]AG
SmlI 46 CTTGAG C^T[CT][GA]AG
StyI 467 CCATGG C^C[AT][AT]GG
Tsp45I 638 GTCAC ^GT[GC]AC
XcmI 587 CCATCTTTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 511 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AfaI 229 GTAC GT^AC
746 GTAC
BsaBI 3 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
141 GATTCTCATC
BsrI 390 ACTGG ACTGG(1/-1)
634 ACTGG
MamI 3 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
141 GATTCTCATC
MslI 36 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
804 CATCTTAATG
MwoI 158 GCATCTCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
644 GCAATCCAAGC
RsaI 229 GTAC GT^AC
746 GTAC
TfiI 141 GATTC G^A[AT]TC
855 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BssKI BstF5I BstNI DdeI EcoRII
Fnu4HI FokI HinfI HphI ItaI
MaeIII MboII MnlI MseI MvaI
NlaIII ScrFI SfaNI Tru9I TseI
Tsp509I TspRI