Restriction map for
pF1KB3547
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaHI BsaI BseAI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstXI
Bsu36I CpoI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AseI 2068 ATTAAT AT^TAAT
AsnI 2068 ATTAAT AT^TAAT
Asp700I 2954 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 127 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2320 GGTGCC G^G[CT][GA]CC
BcnI 2680 CCCGG CC^[GC]GG
BglII 277 AGATCT A^GATCT
BsaAI 1145 TACGTG [CT]AC^GT[GA]
BsaBI 388 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1392 TCCGGT [AT]^CCGG[AT]
BsgI 2687 CTGCAC GTGCAG(16/14)
BsiEI 86 CGGCCG CG[GA][CT]^CG
BspDI 1543 ATCGAT AT^CGAT
BspHI 2553 TCATGA T^CATGA
BspLU11I 3031 ACATGT A^CATGT
BsrFI 1408 ACCGGC [GA]^CCGG[CT]
Bst1107I 201 GTATAC GTA^TAC
BstBI 1664 TTCGAA TT^CGAA
BstZ17I 201 GTATAC GTA^TAC
CfoI 294 GCGC GCG^C
Cfr10I 1408 ACCGGC [GA]^CCGG[CT]
ClaI 1543 ATCGAT AT^CGAT
DraI 2004 TTTAAA TTT^AAA
EagI 86 CGGCCG C^GGCCG
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
EcoT22I 1355 ATGCAT ATGCA^T
HgaI 958 GACGC GACGC(5/10)
HhaI 294 GCGC GCG^C
HinP1I 294 GCGC G^CGC
HincII 1169 GTCAAC GT[CT]^[GA]AC
HindII 1169 GTCAAC GT[CT]^[GA]AC
HindIII 2983 AAGCTT A^AGCTT
MamI 388 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 530 CAATTG C^AATTG
MunI 530 CAATTG C^AATTG
NciI 2680 CCCGG CC^[GC]GG
NruI 588 TCGCGA TCG^CGA
NsiI 1355 ATGCAT ATGCA^T
NspV 1664 TTCGAA TT^CGAA
PflFI 127 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1188 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1770 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2068 ATTAAT AT^TAAT
PstI 2390 CTGCAG CTGCA^G
RcaI 2553 TCATGA T^CATGA
SfuI 1664 TTCGAA TT^CGAA
Tth111I 127 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2954 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 201 GTATAC GT^[AC][GT]AC
367 GTCTAC
AflIII 1146 ACGTGT A^C[GA][CT]GT
3031 ACATGT
BlpI 1321 GCTAAGC GC^T[ACGT]AGC
2714 GCTCAGC
BpmI 2121 CTGGAG CTGGAG(16/14)
2207 CTGGAG
Bpu1102I 1321 GCTAAGC GC^T[ACGT]AGC
2714 GCTCAGC
BseRI 70 GAGGAG GAGGAG(10/8)
1091 GAGGAG
BsmFI 1368 GGGAC GGGAC(10/14)
3028 GGGAC
BspMI 2388 ACCTGC ACCTGC(4/8)
615 GCAGGT
BstYI 277 AGATCT [GA]^GATC[CT]
2457 AGATCC
CelII 1321 GCTAAGC GC^T[ACGT]AGC
2714 GCTCAGC
DraII 1188 AGGACCT [GA]G^G[ACGT]CC[CT]
3126 AGGGCCT
DsaI 664 CCATGG C^C[GA][CT]GG
724 CCATGG
EaeI 86 CGGCCG [CT]^GGCC[GA]
1410 CGGCCA
EcoO109I 1188 AGGACCT [GA]G^G[ACGT]CC[CT]
3126 AGGGCCT
MflI 277 AGATCT [GA]^GATC[CT]
2457 AGATCC
NcoI 664 CCATGG C^CATGG
724 CCATGG
NdeI 2237 CATATG CA^TATG
2720 CATATG
NspI 758 ACATGC [GA]CATG^[CT]
3031 ACATGT
SexAI 2316 ACCTGGT A^CC[AT]GGT
2618 ACCTGGT
SspI 1274 AATATT AAT^ATT
3003 AATATT
XcmI 655 CCAGCTTCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1443 CCAGCTGATTCTTGG
XhoII 277 AGATCT [GA]^GATC[CT]
2457 AGATCC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I