Restriction map for
pF1KE2575
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI Alw44I AlwI AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NaeI NarI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 223 GACGCC G[GA]^CG[CT]C
AhdI 475 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 168 CCTGAGG CC^T[ACGT]AGG
ApaI 246 GGGCCC GGGCC^C
AspEI 475 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BanII 246 GGGCCC G[GA]GC[CT]^C
BbsI 265 GTCTTC GAAGAC(2/6)
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
BmyI 246 GGGCCC G[AGT]GC[ACT]^C
BpmI 416 CTCCAG CTGGAG(16/14)
BpuAI 265 GTCTTC GAAGAC(2/6)
BsaHI 223 GACGCC G[GA]^CG[CT]C
BsaI 175 GAGACC GGTCTC(1/5)
BsmBI 509 GAGACG CGTCTC(1/5)
Bsp1286I 246 GGGCCC G[AGT]GC[ACT]^C
BstAPI 149 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 452 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 168 CCTGAGG CC^T[ACGT]AGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eam1105I 475 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 168 CCTGAGG CC^T[ACGT]AGG
EcoNI 393 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 24 CCGG C^CGG
HgaI 223 GACGC GACGC(5/10)
Hin1I 223 GACGCC G[GA]^CG[CT]C
HinfI 138 GAATC G^A[ACGT]TC
HpaII 24 CCGG C^CGG
MaeIII 483 GTCAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 24 CCGG C^CGG
MwoI 149 GCATCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 335 GCATGT [GA]CATG^[CT]
PpuMI 19 GGGACCC [GA]G^G[AT]CC[CT]
SfaNI 149 GCATC GCATC(5/9)
SmaI 23 CCCGGG CCC^GGG
TfiI 138 GAATC G^A[AT]TC
Tsp45I 483 GTCAC ^GT[GC]AC
TspRI 485 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 135 CCAGAATCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 23 CCCGGG C^[CT]CG[GA]G
307 CCCGAG
AvaII 20 GGACC G^G[AT]CC
456 GGACC
BcnI 23 CCCGG CC^[GC]GG
24 CCGGG
BseRI 211 GAGGAG GAGGAG(10/8)
214 GAGGAG
BsmAI 175 GAGAC GTCTC(1/5)
509 GAGAC
BsoBI 23 CCCGGG C^[CT]CG[GA]G
307 CCCGAG
BstF5I 318 CATCC GGATG(2/0)
372 GGATG
BstNI 399 CCAGG CC^[AT]GG
459 CCTGG
Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC
48 GCAGGC
DsaI 452 CCATGG C^C[GA][CT]GG
504 CCATGG
EcoRII 399 CCAGG ^CC[AT]GG
459 CCTGG
EcoT14I 452 CCATGG C^C[AT][AT]GG
504 CCATGG
FauI 16 GCGGG CCCGC(4/6)
463 GCGGG
FokI 318 CATCC GGATG(9/13)
372 GGATG
MaeII 57 ACGT A^CGT
71 ACGT
MboII 64 TCTTC GAAGA(8/7)
266 TCTTC
MvaI 399 CCAGG CC^[AT]GG
459 CCTGG
NciI 23 CCCGG CC^[GC]GG
24 CCGGG
NcoI 452 CCATGG C^CATGG
504 CCATGG
StyI 452 CCATGG C^C[AT][AT]GG
504 CCATGG
TaqI 131 TCGA T^CGA
473 TCGA
TthHB8I 131 TCGA T^CGA
473 TCGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmFI BssKI Cfr13I DdeI
DraII EcoO109I Fnu4HI HaeIII HphI
ItaI MnlI MspA1I NlaIII NlaIV
PvuII Sau96I ScrFI TseI