Restriction map for
pF1KE2444
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BpmI BsaAI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Cac8I CfoI Cfr10I
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 15 GGCGTC G[GA]^CG[CT]C
AflIII 356 ACATGT A^C[GA][CT]GT
AlwI 40 GGATC GGATC(4/5)
AocI 1036 CCTCAGG CC^T[ACGT]AGG
Asp700I 1167 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1267 GAGCCC G[GA]GC[CT]^C
BbvI 1233 GCAGC GCAGC(8/12)
BcnI 12 CCCGG CC^[GC]GG
BglII 80 AGATCT A^GATCT
BlpI 696 GCTTAGC GC^T[ACGT]AGC
BmyI 1267 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 696 GCTTAGC GC^T[ACGT]AGC
BsaBI 517 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 15 GGCGTC G[GA]^CG[CT]C
BsgI 1181 CTGCAC GTGCAG(16/14)
BsmBI 17 CGTCTC CGTCTC(1/5)
Bsp1286I 1267 GAGCCC G[AGT]GC[ACT]^C
BspDI 400 ATCGAT AT^CGAT
BspLU11I 356 ACATGT A^CATGT
BspMI 482 ACCTGC ACCTGC(4/8)
BstYI 80 AGATCT [GA]^GATC[CT]
Bsu36I 1036 CCTCAGG CC^T[ACGT]AGG
CelII 696 GCTTAGC GC^T[ACGT]AGC
ClaI 400 ATCGAT AT^CGAT
EarI 958 GAAGAG CTCTTC(1/4)
Eco81I 1036 CCTCAGG CC^T[ACGT]AGG
EcoNI 1237 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 368 GAATTC G^AATTC
Fnu4HI 1233 GCAGC GC^[ACGT]GC
HaeIII 1260 GGCC GG^CC
HapII 13 CCGG C^CGG
HgaI 16 GCGTC GACGC(5/10)
Hin1I 15 GGCGTC G[GA]^CG[CT]C
HincII 1116 GTTGAC GT[CT]^[GA]AC
HindII 1116 GTTGAC GT[CT]^[GA]AC
HindIII 725 AAGCTT A^AGCTT
HpaII 13 CCGG C^CGG
ItaI 1233 GCAGC GC^[ACGT]GC
Ksp632I 958 GAAGAG CTCTTC(1/4)
MaeII 35 ACGT A^CGT
MamI 517 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 80 AGATCT [GA]^GATC[CT]
MspI 13 CCGG C^CGG
MwoI 1233 GCAGCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 12 CCCGG CC^[GC]GG
NcoI 856 CCATGG C^CATGG
NlaIV 1315 GGTCCC GG[ACGT]^[ACGT]CC
NspI 356 ACATGT [GA]CATG^[CT]
PflMI 1271 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1022 AGTACT AGT^ACT
SfaNI 108 GCATC GCATC(5/9)
TseI 1233 GCAGC G^C[AT]GC
Van91I 1271 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 80 AGATCT [GA]^GATC[CT]
XmnI 1167 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 1159 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1252 CAGCTCCTG
AvaII 1280 GGACC G^G[AT]CC
1315 GGTCC
BbsI 508 GAAGAC GAAGAC(2/6)
643 GAAGAC
BpuAI 508 GAAGAC GAAGAC(2/6)
643 GAAGAC
BsmAI 18 GTCTC GTCTC(1/5)
588 GTCTC
BsmFI 10 GTCCC GGGAC(10/14)
1316 GTCCC
BspHI 372 TCATGA T^CATGA
1017 TCATGA
BstF5I 1177 CATCC GGATG(2/0)
932 GGATG
BstNI 1237 CCTGG CC^[AT]GG
1257 CCTGG
Cfr13I 1280 GGACC G^G[ACGT]CC
1315 GGTCC
DsaI 334 CCGTGG C^C[GA][CT]GG
856 CCATGG
EcoRII 1237 CCTGG ^CC[AT]GG
1257 CCTGG
EcoT14I 230 CCTTGG C^C[AT][AT]GG
856 CCATGG
FokI 1177 CATCC GGATG(9/13)
932 GGATG
MfeI 490 CAATTG C^AATTG
702 CAATTG
MslI 115 CATAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
835 CATTTTTATG
MspA1I 526 CAGCTG C[AC]G^C[GT]G
1272 CAGCTG
MunI 490 CAATTG C^AATTG
702 CAATTG
MvaI 1237 CCTGG CC^[AT]GG
1257 CCTGG
PstI 553 CTGCAG CTGCA^G
1231 CTGCAG
PvuII 526 CAGCTG CAG^CTG
1272 CAGCTG
RcaI 372 TCATGA T^CATGA
1017 TCATGA
Sau96I 1280 GGACC G^G[ACGT]CC
1315 GGTCC
SspI 789 AATATT AAT^ATT
1299 AATATT
StyI 230 CCTTGG C^C[AT][AT]GG
856 CCATGG
Tsp45I 450 GTCAC ^GT[GC]AC
1124 GTGAC
TspRI 563 CACTG CA[GC]TG[ACGT][ACGT]^
1284 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsaJI BsiYI BslI BsrDI BsrI
BssKI DdeI DpnII DraI HinfI
HphI MaeI MaeIII MboI MboII
MnlI MseI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfcI TaqI
TfiI Tru9I Tsp509I TthHB8I