Restriction map for
pF1KE2445
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AflII 97 CTTAAG C^TTAAG
AflIII 1165 ACGTGT A^C[GA][CT]GT
AhdI 132 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 196 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 997 CCTGAGG CC^T[ACGT]AGG
AspEI 132 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 26 TGGCCA TGG^CCA
BanI 1079 GGCACC G^G[CT][GA]CC
BfrI 97 CTTAAG C^TTAAG
BmyI 1271 GGGCAC G[AGT]GC[ACT]^C
BsaI 435 GAGACC GGTCTC(1/5)
BseRI 13 CTCCTC GAGGAG(10/8)
BsgI 516 CTGCAC GTGCAG(16/14)
Bsp1286I 1271 GGGCAC G[AGT]GC[ACT]^C
Bsu36I 997 CCTGAGG CC^T[ACGT]AGG
DraI 1005 TTTAAA TTT^AAA
DraII 503 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 132 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 997 CCTGAGG CC^T[ACGT]AGG
EcoO109I 503 AGGACCT [GA]G^G[ACGT]CC[CT]
MluNI 26 TGGCCA TGG^CCA
MscI 26 TGGCCA TGG^CCA
MwoI 1003 GCTTTAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 1079 GGCACC GG[ACGT]^[ACGT]CC
PpuMI 503 AGGACCT [GA]G^G[AT]CC[CT]
SphI 233 GCATGC GCATG^C
Tsp45I 319 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 1278 CCGC CCGC(-3/-1)
1248 GCGG
AfaI 305 GTAC GT^AC
790 GTAC
AlwI 198 GATCC GGATC(4/5)
664 GATCC
Asp700I 979 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1210 GAAAAACTTC
BsaBI 538 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATTGTCATC
BsaJI 23 CCGTGG C^C[ACGT][ACGT]GG
579 CCACGG
BspHI 1035 TCATGA T^CATGA
1202 TCATGA
DsaI 23 CCGTGG C^C[GA][CT]GG
579 CCACGG
EaeI 26 TGGCCA [CT]^GGCC[GA]
1185 TGGCCG
HindIII 144 AAGCTT A^AGCTT
600 AAGCTT
MaeII 138 ACGT A^CGT
1165 ACGT
MaeIII 319 GTGAC ^GT[ACGT]AC
595 GTAAC
MamI 538 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1293 GATTGTCATC
RcaI 1035 TCATGA T^CATGA
1202 TCATGA
RsaI 305 GTAC GT^AC
790 GTAC
XbaI 750 TCTAGA T^CTAGA
897 TCTAGA
XmnI 979 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1210 GAAAAACTTC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BfaI BglII BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MslI MvaI
NdeII NlaIII NspI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI TfiI
Tru9I TseI Tsp509I TspRI XhoII