Restriction map for
pF1KE2665
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BanI BanII BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tth111I Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 387 GCGG CCGC(-3/-1)
AflIII 806 ACATGT A^C[GA][CT]GT
AseI 673 ATTAAT AT^TAAT
AsnI 673 ATTAAT AT^TAAT
Asp700I 575 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 764 CTCGAG C^[CT]CG[GA]G
BamHI 685 GGATCC G^GATCC
BbsI 660 GTCTTC GAAGAC(2/6)
BclI 536 TGATCA T^GATCA
BmyI 57 GGGCAC G[AGT]GC[ACT]^C
BpmI 1111 CTGGAG CTGGAG(16/14)
BpuAI 660 GTCTTC GAAGAC(2/6)
BsiYI 54 CCTGGGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 54 CCTGGGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 600 GTCCC GGGAC(10/14)
BsoBI 764 CTCGAG C^[CT]CG[GA]G
Bsp1286I 57 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 806 ACATGT A^CATGT
BspMI 914 ACCTGC ACCTGC(4/8)
BsrDI 144 CATTGC GCAATG(2/0)
Bst1107I 355 GTATAC GTA^TAC
BstZ17I 355 GTATAC GTA^TAC
DraII 911 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 201 GACAGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 911 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 252 GAATTC G^AATTC
EcoT14I 66 CCTTGG C^C[AT][AT]GG
EcoT22I 45 ATGCAT ATGCA^T
FauI 387 GCGGG CCCGC(4/6)
FbaI 536 TGATCA T^GATCA
HincII 1044 GTTGAC GT[CT]^[GA]AC
HindII 1044 GTTGAC GT[CT]^[GA]AC
HindIII 1088 AAGCTT A^AGCTT
NsiI 45 ATGCAT ATGCA^T
NspI 806 ACATGT [GA]CATG^[CT]
PaeR7I 764 CTCGAG C^TCGAG
PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 673 ATTAAT AT^TAAT
PstI 134 CTGCAG CTGCA^G
ScaI 343 AGTACT AGT^ACT
SfcI 134 CTGCAG C^T[GA][CT]AG
SmlI 764 CTCGAG C^T[CT][GA]AG
StyI 66 CCTTGG C^C[AT][AT]GG
XbaI 873 TCTAGA T^CTAGA
XhoI 764 CTCGAG C^TCGAG
XmnI 575 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 355 GTATAC GT^[AC][GT]AC
866 GTAGAC
AcsI 18 GAATTT [GA]^AATT[CT]
252 GAATTC
ApoI 18 GAATTT [GA]^AATT[CT]
252 GAATTC
BbvCI 1102 CCTCAGC CCTCAGC(-5/-2)
3 GCTGAGG
BseRI 761 CTCCTC GAGGAG(10/8)
71 GAGGAG
BsgI 559 GTGCAG GTGCAG(16/14)
742 GTGCAG
BsmAI 767 GAGAC GTCTC(1/5)
803 GAGAC
BssKI 54 CCTGG ^CC[ACGT]GG
423 CCTGG
BstNI 54 CCTGG CC^[AT]GG
423 CCTGG
Cac8I 744 GCAGGC GC[ACGT]^[ACGT]GC
956 GCCTGC
EcoRII 54 CCTGG ^CC[AT]GG
423 CCTGG
MaeIII 179 GTAAC ^GT[ACGT]AC
926 GTAAC
MvaI 54 CCTGG CC^[AT]GG
423 CCTGG
NlaIV 389 GGGTCC GG[ACGT]^[ACGT]CC
685 GGATCC
ScrFI 54 CCTGG CC^[ACGT]GG
423 CCTGG
SfaNI 714 GATGC GCATC(5/9)
47 GCATC
TaqI 765 TCGA T^CGA
897 TCGA
TthHB8I 765 TCGA T^CGA
897 TCGA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AvaII
BbvI BfaI BsaJI BsrI BstYI
Cfr13I DdeI DpnII Fnu4HI HaeIII
ItaI MaeI MboI MboII MflI
MnlI MseI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I StuI Tru9I
TseI Tsp509I TspRI XhoII