Restriction map for
pF1KE2663
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BalI
BamHI BbrPI BcgI BciVI BfrI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FauI
FseI FspI HgaI HpaI KpnI
Ksp632I KspI MaeII MamI MluI
MluNI MroI MscI MvnI NdeI
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AcsI 960 GAATTT [GA]^AATT[CT]
AcyI 122 GGCGCC G[GA]^CG[CT]C
AlwI 1266 GGATC GGATC(4/5)
ApoI 960 GAATTT [GA]^AATT[CT]
AvrII 1258 CCTAGG C^CTAGG
BanI 122 GGCGCC G^G[CT][GA]CC
BbeI 122 GGCGCC GGCGC^C
BbvCI 81 CCTCAGC CCTCAGC(-5/-2)
BclI 875 TGATCA T^GATCA
BglI 114 GCCCATCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1393 AGATCT A^GATCT
BlnI 1258 CCTAGG C^CTAGG
BsaHI 122 GGCGCC G[GA]^CG[CT]C
BsaI 460 GAGACC GGTCTC(1/5)
BseRI 679 GAGGAG GAGGAG(10/8)
BsiEI 626 CGATCG CG[GA][CT]^CG
BsmBI 89 CGTCTC CGTCTC(1/5)
BsmFI 1208 GTCCC GGGAC(10/14)
BsmI 360 GCATTC GAATGC(1/-1)
Bsp1407I 551 TGTACA T^GTACA
BspHI 441 TCATGA T^CATGA
BsrDI 866 CATTGC GCAATG(2/0)
BsrFI 125 GCCGGC [GA]^CCGG[CT]
BsrGI 551 TGTACA T^GTACA
BstBI 659 TTCGAA TT^CGAA
BstYI 1393 AGATCT [GA]^GATC[CT]
Cac8I 125 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 123 GCGC GCG^C
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
DraII 1206 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 38 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1206 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 484 GATATC GAT^ATC
FbaI 875 TGATCA T^GATCA
HaeII 122 GGCGCC [GA]GCGC^[CT]
HhaI 123 GCGC GCG^C
Hin1I 122 GGCGCC G[GA]^CG[CT]C
HinP1I 123 GCGC G^CGC
HindIII 1375 AAGCTT A^AGCTT
HphI 392 TCACC GGTGA(8/7)
KasI 122 GGCGCC G^GCGCC
MfeI 836 CAATTG C^AATTG
MflI 1393 AGATCT [GA]^GATC[CT]
MslI 471 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 807 CAGCGG C[AC]G^C[GT]G
MunI 836 CAATTG C^AATTG
NaeI 125 GCCGGC GCC^GGC
NarI 122 GGCGCC GG^CGCC
NcoI 395 CCATGG C^CATGG
NgoMIV 125 GCCGGC G^CCGGC
NspV 659 TTCGAA TT^CGAA
PaeR7I 606 CTCGAG C^TCGAG
PpuMI 1206 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1201 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 626 CGATCG CGAT^CG
RcaI 441 TCATGA T^CATGA
SacI 594 GAGCTC GAGCT^C
SfoI 122 GGCGCC GGC^GCC
SfuI 659 TTCGAA TT^CGAA
SmaI 131 CCCGGG CCC^GGG
SmlI 606 CTCGAG C^T[CT][GA]AG
SspBI 551 TGTACA T^GTACA
XhoI 606 CTCGAG C^TCGAG
XhoII 1393 AGATCT [GA]^GATC[CT]
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 552 GTAC GT^AC
1074 GTAC
AspHI 205 GTGCTC G[AT]GC[AT]^C
594 GAGCTC
BanII 594 GAGCTC G[GA]GC[CT]^C
904 GAGCCC
BbsI 1136 GAAGAC GAAGAC(2/6)
1309 GAAGAC
BfaI 1259 CTAG C^TAG
1297 CTAG
BpuAI 1136 GAAGAC GAAGAC(2/6)
1309 GAAGAC
BsiHKAI 205 GTGCTC G[AT]GC[AT]^C
594 GAGCTC
BstAPI 351 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
720 GCATCTTATGC
DsaI 395 CCATGG C^C[GA][CT]GG
674 CCGTGG
EaeI 32 TGGCCG [CT]^GGCC[GA]
233 TGGCCG
HincII 76 GTCAAC GT[CT]^[GA]AC
291 GTCAAC
HindII 76 GTCAAC GT[CT]^[GA]AC
291 GTCAAC
MaeI 1259 CTAG C^TAG
1297 CTAG
MseI 697 TTAA T^TAA
884 TTAA
NspI 72 GCATGT [GA]CATG^[CT]
548 GCATGT
RsaI 552 GTAC GT^AC
1074 GTAC
Tru9I 697 TTAA T^TAA
884 TTAA
Tsp45I 452 GTGAC ^GT[GC]AC
1176 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp509I
TspRI TthHB8I