Restriction map for
pF1KE2516
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MfeI
MluI MluNI MroI MscI MunI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp509I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 1018 ACGTGT A^C[GA][CT]GT
Asp700I 156 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 386 GTGCTC G[AT]GC[AT]^C
AviII 376 TGCGCA TGC^GCA
BanI 457 GGCGCC G^G[CT][GA]CC
BbeI 457 GGCGCC GGCGC^C
BfaI 248 CTAG C^TAG
BsaBI 673 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 706 GAGACC GGTCTC(1/5)
BsgI 617 CTGCAC GTGCAG(16/14)
BsiHKAI 386 GTGCTC G[AT]GC[AT]^C
BsmAI 706 GAGAC GTCTC(1/5)
BsmFI 609 GTCCC GGGAC(10/14)
BsmI 925 GAATGC GAATGC(1/-1)
Bsp1407I 1002 TGTACA T^GTACA
BsrDI 800 GCAATG GCAATG(2/0)
BsrGI 1002 TGTACA T^GTACA
BstYI 1008 AGATCC [GA]^GATC[CT]
DraIII 652 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 54 CGGCCG [CT]^GGCC[GA]
EagI 54 CGGCCG C^GGCCG
EclXI 54 CGGCCG C^GGCCG
Eco52I 54 CGGCCG C^GGCCG
EcoNI 871 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 376 TGCGCA TGC^GCA
KasI 457 GGCGCC G^GCGCC
KspI 87 CCGCGG CCGC^GG
MaeI 248 CTAG C^TAG
MaeIII 1097 GTGAC ^GT[ACGT]AC
MamI 673 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1008 AGATCC [GA]^GATC[CT]
MseI 1075 TTAA T^TAA
MslI 843 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 87 CCGCGG C[AC]G^C[GT]G
NarI 457 GGCGCC GG^CGCC
NcoI 623 CCATGG C^CATGG
NheI 247 GCTAGC G^CTAGC
SacII 87 CCGCGG CCGC^GG
SfcI 907 CTACAG C^T[GA][CT]AG
SfoI 457 GGCGCC GGC^GCC
SmaI 419 CCCGGG CCC^GGG
SmlI 1069 CTTGAG C^T[CT][GA]AG
SspBI 1002 TGTACA T^GTACA
TfiI 645 GAATC G^A[AT]TC
Tru9I 1075 TTAA T^TAA
Tsp45I 1097 GTGAC ^GT[GC]AC
XhoII 1008 AGATCC [GA]^GATC[CT]
XmaCI 419 CCCGGG C^CCGGG
XmaI 419 CCCGGG C^CCGGG
XmnI 156 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 145 GGCGTC G[GA]^CG[CT]C
457 GGCGCC
AlwI 1009 GATCC GGATC(4/5)
863 GGATC
AlwNI 409 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
687 CAGCTACTG
AvaII 204 GGTCC G^G[AT]CC
608 GGTCC
BglI 318 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
509 GCCGCGCCGGC
BpmI 389 CTCCAG CTGGAG(16/14)
814 CTGGAG
BsaHI 145 GGCGTC G[GA]^CG[CT]C
457 GGCGCC
BsiEI 54 CGGCCG CG[GA][CT]^CG
522 CGGTCG
BsrFI 56 GCCGGC [GA]^CCGG[CT]
514 GCCGGC
BssHII 397 GCGCGC G^CGCGC
399 GCGCGC
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
514 GCCGGC
DdeI 830 CTGAG C^T[ACGT]AG
978 CTCAG
DraII 410 AGGCCCT [GA]G^G[ACGT]CC[CT]
809 AGGGCCT
EcoO109I 410 AGGCCCT [GA]G^G[ACGT]CC[CT]
809 AGGGCCT
EcoT14I 623 CCATGG C^C[AT][AT]GG
876 CCAAGG
HaeII 334 GGCGCT [GA]GCGC^[CT]
457 GGCGCC
HgaI 73 GACGC GACGC(5/10)
146 GCGTC
Hin1I 145 GGCGTC G[GA]^CG[CT]C
457 GGCGCC
MaeII 1018 ACGT A^CGT
1083 ACGT
NaeI 56 GCCGGC GCC^GGC
514 GCCGGC
NgoMIV 56 GCCGGC G^CCGGC
514 GCCGGC
PflMI 168 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCAGGCCCTGG
SfaNI 780 GATGC GCATC(5/9)
785 GATGC
StyI 623 CCATGG C^C[AT][AT]GG
876 CCAAGG
TaqI 704 TCGA T^CGA
746 TCGA
TthHB8I 704 TCGA T^CGA
746 TCGA
Van91I 168 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsoBI Bsp1286I
BsrBI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DsaI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TseI TspRI