Restriction map for
pF1KE2346
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI ApaI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbeI BbrPI BcnI BfrI
BlnI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I FseI
FspI HgaI KasI KpnI KspI
MluI MroI MvnI NarI NciI
NcoI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SfiI
SfoI SgrAI SmaI SnaBI SwaI
Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 8116 GACGTC GACGT^C
AcyI 8116 GACGTC G[GA]^CG[CT]C
Alw44I 751 GTGCAC G^TGCAC
ApaLI 751 GTGCAC G^TGCAC
AseI 1614 ATTAAT AT^TAAT
AsnI 1614 ATTAAT AT^TAAT
AvaI 920 CTCGAG C^[CT]CG[GA]G
BbvCI 5527 CCTCAGC CCTCAGC(-5/-2)
BcgI 5802 CGATGTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7014 TGATCA T^GATCA
BsaAI 6737 CACGTA [CT]AC^GT[GA]
BsaHI 8116 GACGTC G[GA]^CG[CT]C
BsmBI 1579 GAGACG CGTCTC(1/5)
BsoBI 920 CTCGAG C^[CT]CG[GA]G
BspHI 3402 TCATGA T^CATGA
DsaI 7197 CCGTGG C^C[GA][CT]GG
FauI 40 GCGGG CCCGC(4/6)
FbaI 7014 TGATCA T^GATCA
Hin1I 8116 GACGTC G[GA]^CG[CT]C
HpaI 6499 GTTAAC GTT^AAC
MfeI 5354 CAATTG C^AATTG
MunI 5354 CAATTG C^AATTG
NaeI 2126 GCCGGC GCC^GGC
NgoMIV 2126 GCCGGC G^CCGGC
NheI 7921 GCTAGC G^CTAGC
PaeR7I 920 CTCGAG C^TCGAG
PshAI 5472 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1614 ATTAAT AT^TAAT
RcaI 3402 TCATGA T^CATGA
SalI 5522 GTCGAC G^TCGAC
SbfI 547 CCTGCAGG CCTGCA^GG
Sse8387I 547 CCTGCAGG CCTGCA^GG
XbaI 7854 TCTAGA T^CTAGA
XhoI 920 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AocI 3682 CCTCAGG CC^T[ACGT]AGG
6812 CCTCAGG
Aor51HI 1466 AGCGCT AGC^GCT
7165 AGCGCT
Asp700I 3413 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6187 GAAACTTTTC
BanI 6343 GGTGCC G^G[CT][GA]CC
8277 GGTGCC
BciVI 553 GGATAC GTATCC(6/5)
5574 GGATAC
BlpI 2995 GCTAAGC GC^T[ACGT]AGC
8251 GCTAAGC
Bpu1102I 2995 GCTAAGC GC^T[ACGT]AGC
8251 GCTAAGC
Bsp1407I 348 TGTACA T^GTACA
3980 TGTACA
BsrFI 458 GCCGGT [GA]^CCGG[CT]
2126 GCCGGC
BsrGI 348 TGTACA T^GTACA
3980 TGTACA
BssSI 1088 CTCGTG CACGAG(-5/-1)
2678 CTCGTG
BstBI 2519 TTCGAA TT^CGAA
5363 TTCGAA
Bsu36I 3682 CCTCAGG CC^T[ACGT]AGG
6812 CCTCAGG
CelII 2995 GCTAAGC GC^T[ACGT]AGC
8251 GCTAAGC
Cfr10I 458 GCCGGT [GA]^CCGG[CT]
2126 GCCGGC
DraII 1386 GGGACCT [GA]G^G[ACGT]CC[CT]
6377 AGGACCC
DraIII 745 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3191 CACTTTGTG
DrdI 271 GACATACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2666 GACCTGACAGTC
Eco47III 1466 AGCGCT AGC^GCT
7165 AGCGCT
Eco81I 3682 CCTCAGG CC^T[ACGT]AGG
6812 CCTCAGG
EcoO109I 1386 GGGACCT [GA]G^G[ACGT]CC[CT]
6377 AGGACCC
EcoRI 390 GAATTC G^AATTC
5991 GAATTC
EcoRV 937 GATATC GAT^ATC
1374 GATATC
HapII 459 CCGG C^CGG
2127 CCGG
HpaII 459 CCGG C^CGG
2127 CCGG
MspI 459 CCGG C^CGG
2127 CCGG
NdeI 3548 CATATG CA^TATG
7060 CATATG
NspV 2519 TTCGAA TT^CGAA
5363 TTCGAA
PflMI 2641 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6051 CCAGCTCATGG
PpuMI 1386 GGGACCT [GA]G^G[AT]CC[CT]
6377 AGGACCC
PvuII 73 CAGCTG CAG^CTG
4736 CAGCTG
SapI 3352 GAAGAGC GCTCTTC(1/4)
7330 GAAGAGC
ScaI 6516 AGTACT AGT^ACT
7697 AGTACT
SexAI 209 ACCAGGT A^CC[AT]GGT
7094 ACCAGGT
SfuI 2519 TTCGAA TT^CGAA
5363 TTCGAA
SphI 332 GCATGC GCATG^C
5815 GCATGC
SspBI 348 TGTACA T^GTACA
3980 TGTACA
Van91I 2641 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6051 CCAGCTCATGG
XmnI 3413 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6187 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BbsI BbvI BfaI
BglI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoNI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII