Restriction map for
pF1KE1499
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# NO CUT ENZYMES ( 29 enzymes) #
##################################
AccIII AgeI AscI AseI AsnI
BseAI BsiWI BspDI BspEI ClaI
CpoI EcoT22I HpaI MluI MroI
NdeI NsiI PacI PinAI PmeI
PshBI PvuI RsrII SalI ScaI
SgrAI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 8711 GGTACC G^GTACC
AclI 264 AACGTT AA^CGTT
AflII 4711 CTTAAG C^TTAAG
Alw44I 1600 GTGCAC G^TGCAC
Aor51HI 2132 AGCGCT AGC^GCT
ApaLI 1600 GTGCAC G^TGCAC
Asp718I 8711 GGTACC G^GTACC
AspI 8503 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 8328 CCTAGG C^CTAGG
BfrI 4711 CTTAAG C^TTAAG
BlnI 8328 CCTAGG C^CTAGG
Bsp1407I 6170 TGTACA T^GTACA
BsrGI 6170 TGTACA T^GTACA
Bst1107I 3512 GTATAC GTA^TAC
BstZ17I 3512 GTATAC GTA^TAC
DrdI 5479 GACGAGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2132 AGCGCT AGC^GCT
EcoRV 3784 GATATC GAT^ATC
FseI 7152 GGCCGGCC GGCCGG^CC
KpnI 8711 GGTACC GGTAC^C
KspI 53 CCGCGG CCGC^GG
MfeI 5735 CAATTG C^AATTG
MunI 5735 CAATTG C^AATTG
NheI 7315 GCTAGC G^CTAGC
NotI 5061 GCGGCCGC GC^GGCCGC
NruI 1265 TCGCGA TCG^CGA
PflFI 8503 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 264 AACGTT AA^CGTT
SacII 53 CCGCGG CCGC^GG
SexAI 8504 ACCAGGT A^CC[AT]GGT
SfiI 7143 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 5928 ACTAGT A^CTAGT
SspBI 6170 TGTACA T^GTACA
Tth111I 8503 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AhdI 2401 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7090 GACAAGATGTC
AocI 739 CCTCAGG CC^T[ACGT]AGG
8319 CCTGAGG
AspEI 2401 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7090 GACAAGATGTC
AviII 2363 TGCGCA TGC^GCA
5318 TGCGCA
BbeI 6985 GGCGCC GGCGC^C
7261 GGCGCC
BsaBI 639 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4911 GATTTCCATC
BsaWI 1961 ACCGGA [AT]^CCGG[AT]
8708 TCCGGT
BstBI 161 TTCGAA TT^CGAA
4502 TTCGAA
BstEII 8355 GGTGACC G^GT[ACGT]ACC
8508 GGTCACC
BstPI 8355 GGTGACC G^GT[ACGT]ACC
8508 GGTCACC
Bsu36I 739 CCTCAGG CC^T[ACGT]AGG
8319 CCTGAGG
DraIII 1624 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5708 CACCGAGTG
EagI 5062 CGGCCG C^GGCCG
5302 CGGCCG
Eam1105I 2401 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7090 GACAAGATGTC
EclXI 5062 CGGCCG C^GGCCG
5302 CGGCCG
Eco52I 5062 CGGCCG C^GGCCG
5302 CGGCCG
Eco81I 739 CCTCAGG CC^T[ACGT]AGG
8319 CCTGAGG
EcoO65I 8355 GGTGACC G^GT[ACGT]ACC
8508 GGTCACC
EcoRI 8056 GAATTC G^AATTC
8869 GAATTC
FspI 2363 TGCGCA TGC^GCA
5318 TGCGCA
KasI 6985 GGCGCC G^GCGCC
7261 GGCGCC
MamI 639 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4911 GATTTCCATC
NarI 6985 GGCGCC GG^CGCC
7261 GGCGCC
NcoI 1130 CCATGG C^CATGG
5615 CCATGG
NspV 161 TTCGAA TT^CGAA
4502 TTCGAA
SfoI 6985 GGCGCC GGC^GCC
7261 GGCGCC
SfuI 161 TTCGAA TT^CGAA
4502 TTCGAA
SnaBI 3948 TACGTA TAC^GTA
8947 TACGTA
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# 3 OR MORE CUT ENZYMES ( 167 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmaI SmlI SphI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI