Restriction map for
pF1KE2426
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I AspI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BclI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BspDI BspEI BsrBI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SphI StuI SwaI Tth111I
Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1812 GGTACC G^GTACC
AciI 10 CCGC CCGC(-3/-1)
AflII 1614 CTTAAG C^TTAAG
AflIII 1771 ACATGT A^C[GA][CT]GT
AhdI 1549 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 674 GTGCAC G^TGCAC
ApaI 1663 GGGCCC GGGCC^C
ApaLI 674 GTGCAC G^TGCAC
Asp718I 1812 GGTACC G^GTACC
AspEI 1549 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 601 GCTGAGG CCTCAGC(-5/-2)
BfrI 1614 CTTAAG C^TTAAG
BseRI 1826 GAGGAG GAGGAG(10/8)
BsgI 224 GTGCAG GTGCAG(16/14)
BsmFI 528 GGGAC GGGAC(10/14)
BsmI 1043 GCATTC GAATGC(1/-1)
Bsp1407I 1758 TGTACA T^GTACA
BspLU11I 1771 ACATGT A^CATGT
BspMI 1833 ACCTGC ACCTGC(4/8)
BsrDI 1207 GCAATG GCAATG(2/0)
BsrFI 1599 GCCGGC [GA]^CCGG[CT]
BsrGI 1758 TGTACA T^GTACA
BstAPI 226 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1599 GCCGGC [GA]^CCGG[CT]
DraI 370 TTTAAA TTT^AAA
EaeI 1048 CGGCCA [CT]^GGCC[GA]
Eam1105I 1549 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 379 CTCTTC CTCTTC(1/4)
EcoRI 464 GAATTC G^AATTC
KpnI 1812 GGTACC GGTAC^C
Ksp632I 379 CTCTTC CTCTTC(1/4)
NaeI 1599 GCCGGC GCC^GGC
NcoI 744 CCATGG C^CATGG
NgoMIV 1599 GCCGGC G^CCGGC
NheI 12 GCTAGC G^CTAGC
ScaI 1380 AGTACT AGT^ACT
SmaI 149 CCCGGG CCC^GGG
SpeI 1255 ACTAGT A^CTAGT
SspBI 1758 TGTACA T^GTACA
SspI 1798 AATATT AAT^ATT
Tsp45I 721 GTCAC ^GT[GC]AC
XbaI 978 TCTAGA T^CTAGA
XmaCI 149 CCCGGG C^CCGGG
XmaI 149 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 580 GTATAC GT^[AC][GT]AC
1628 GTATAC
AlwNI 227 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
596 CAGCTGCTG
AvaI 149 CCCGGG C^[CT]CG[GA]G
1666 CCCGAG
AvaII 606 GGTCC G^G[AT]CC
1831 GGACC
BanI 6 GGTGCC G^G[CT][GA]CC
1812 GGTACC
BbsI 430 GAAGAC GAAGAC(2/6)
99 GTCTTC
BciVI 659 GGATAC GTATCC(6/5)
1518 GTATCC
BcnI 149 CCCGG CC^[GC]GG
150 CCGGG
BpuAI 430 GAAGAC GAAGAC(2/6)
99 GTCTTC
BsiYI 150 CCGGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1816 CCTGAAGGTGG
BslI 150 CCGGGATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1816 CCTGAAGGTGG
BsoBI 149 CCCGGG C^[CT]CG[GA]G
1666 CCCGAG
BspHI 615 TCATGA T^CATGA
1584 TCATGA
Bst1107I 580 GTATAC GTA^TAC
1628 GTATAC
BstYI 916 GGATCT [GA]^GATC[CT]
1785 AGATCC
BstZ17I 580 GTATAC GTA^TAC
1628 GTATAC
HapII 150 CCGG C^CGG
1600 CCGG
HpaII 150 CCGG C^CGG
1600 CCGG
MflI 916 GGATCT [GA]^GATC[CT]
1785 AGATCC
MspA1I 248 CAGCTG C[AC]G^C[GT]G
596 CAGCTG
MspI 150 CCGG C^CGG
1600 CCGG
NciI 149 CCCGG CC^[GC]GG
150 CCGGG
NspI 711 ACATGC [GA]CATG^[CT]
1771 ACATGT
PpuMI 605 AGGTCCT [GA]G^G[AT]CC[CT]
1830 AGGACCT
PvuII 248 CAGCTG CAG^CTG
596 CAGCTG
RcaI 615 TCATGA T^CATGA
1584 TCATGA
SacI 329 GAGCTC GAGCT^C
1091 GAGCTC
SbfI 232 CCTGCAGG CCTGCA^GG
1684 CCTGCAGG
SmlI 1486 CTCAAG C^T[CT][GA]AG
1614 CTTAAG
Sse8387I 232 CCTGCAGG CCTGCA^GG
1684 CCTGCAGG
TaqI 120 TCGA T^CGA
1651 TCGA
TthHB8I 120 TCGA T^CGA
1651 TCGA
XhoII 916 GGATCT [GA]^GATC[CT]
1785 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BanII BbvI BfaI BmyI
BsaJI BsiHKAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI