Restriction map for
pF1KB0468
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI ApaI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 532 CGCG CG^CG
AcyI 523 GGCGTC G[GA]^CG[CT]C
AhdI 1693 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 673 GTGCAC G^TGCAC
AlwNI 293 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2196 AGCGCT AGC^GCT
ApaLI 673 GTGCAC G^TGCAC
AspEI 1693 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2494 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1051 GGATCC G^GATCC
BbsI 1988 GTCTTC GAAGAC(2/6)
BbvCI 2173 CCTCAGC CCTCAGC(-5/-2)
BciVI 1719 GTATCC GTATCC(6/5)
BcnI 1432 CCCGG CC^[GC]GG
BlpI 2312 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2312 GCTTAGC GC^T[ACGT]AGC
BpuAI 1988 GTCTTC GAAGAC(2/6)
BsaBI 1714 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 523 GGCGTC G[GA]^CG[CT]C
BsgI 752 CTGCAC GTGCAG(16/14)
BsiEI 1609 CGATCG CG[GA][CT]^CG
BsmI 1299 GCATTC GAATGC(1/-1)
BspMI 2810 ACCTGC ACCTGC(4/8)
BsrBI 2932 CCGCTC CCGCTC(-3/-3)
BssHII 531 GCGCGC G^CGCGC
BstBI 939 TTCGAA TT^CGAA
BstUI 532 CGCG CG^CG
CelII 2312 GCTTAGC GC^T[ACGT]AGC
DrdI 50 GACGGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 311 CCATGG C^C[GA][CT]GG
Eam1105I 1693 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2509 GAAGAG CTCTTC(1/4)
Eco47III 2196 AGCGCT AGC^GCT
HaeII 2196 AGCGCT [GA]GCGC^[CT]
HgaI 524 GCGTC GACGC(5/10)
Hin1I 523 GGCGTC G[GA]^CG[CT]C
Ksp632I 2509 GAAGAG CTCTTC(1/4)
MamI 1714 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 532 CGCG CG^CG
NciI 1432 CCCGG CC^[GC]GG
NcoI 311 CCATGG C^CATGG
NheI 760 GCTAGC G^CTAGC
NspV 939 TTCGAA TT^CGAA
PflFI 2494 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2644 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1609 CGATCG CGAT^CG
SacI 507 GAGCTC GAGCT^C
SexAI 2798 ACCTGGT A^CC[AT]GGT
SfuI 939 TTCGAA TT^CGAA
Tth111I 2494 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2644 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Asp700I 1732 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1769 GAAGTGTTTC
BalI 441 TGGCCA TGG^CCA
1167 TGGCCA
BanI 1039 GGCACC G^G[CT][GA]CC
1312 GGCACC
BanII 507 GAGCTC G[GA]GC[CT]^C
771 GGGCTC
BsaI 4 GAGACC GGTCTC(1/5)
1619 GAGACC
BspHI 561 TCATGA T^CATGA
2612 TCATGA
BssSI 2053 CACGAG CACGAG(-5/-1)
2192 CACGAG
BstXI 980 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2774 CCAAGCTGCTGG
DraI 1321 TTTAAA TTT^AAA
2368 TTTAAA
DraII 93 GGGACCT [GA]G^G[ACGT]CC[CT]
1112 AGGTCCC
EaeI 441 TGGCCA [CT]^GGCC[GA]
1167 TGGCCA
EcoO109I 93 GGGACCT [GA]G^G[ACGT]CC[CT]
1112 AGGTCCC
EcoRI 1548 GAATTC G^AATTC
2818 GAATTC
EcoT14I 311 CCATGG C^C[AT][AT]GG
2906 CCAAGG
EcoT22I 2518 ATGCAT ATGCA^T
2791 ATGCAT
HincII 136 GTCAAC GT[CT]^[GA]AC
2155 GTCAAC
HindII 136 GTCAAC GT[CT]^[GA]AC
2155 GTCAAC
MaeII 140 ACGT A^CGT
2125 ACGT
MluNI 441 TGGCCA TGG^CCA
1167 TGGCCA
MscI 441 TGGCCA TGG^CCA
1167 TGGCCA
MslI 832 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2607 CACCATCATG
NsiI 2518 ATGCAT ATGCA^T
2791 ATGCAT
NspI 706 ACATGC [GA]CATG^[CT]
756 ACATGC
PpuMI 93 GGGACCT [GA]G^G[AT]CC[CT]
1112 AGGTCCC
PstI 834 CTGCAG CTGCA^G
2584 CTGCAG
RcaI 561 TCATGA T^CATGA
2612 TCATGA
ScaI 175 AGTACT AGT^ACT
982 AGTACT
SspI 904 AATATT AAT^ATT
1177 AATATT
StyI 311 CCATGG C^C[AT][AT]GG
2906 CCAAGG
XbaI 36 TCTAGA T^CTAGA
1702 TCTAGA
XcmI 1569 CCACTACAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2789 CCATGCATGACCTGG
XmnI 1732 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1769 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII