Restriction map for
pF1KE2506
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 333 AAATTC [GA]^AATT[CT]
ApoI 333 AAATTC [GA]^AATT[CT]
Asp700I 217 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1189 CTCGAG C^[CT]CG[GA]G
BalI 1176 TGGCCA TGG^CCA
BamHI 621 GGATCC G^GATCC
BcgI 749 GCAGTAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 557 GTATCC GTATCC(6/5)
BglII 288 AGATCT A^GATCT
BsaAI 395 TACGTG [CT]AC^GT[GA]
BsoBI 1189 CTCGAG C^[CT]CG[GA]G
BspHI 420 TCATGA T^CATGA
BsrBI 257 CCGCTC CCGCTC(-3/-3)
Bst1107I 522 GTATAC GTA^TAC
BstXI 702 CCAGTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 522 GTATAC GTA^TAC
Cfr13I 900 GGCCC G^G[ACGT]CC
DraI 886 TTTAAA TTT^AAA
DraII 899 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 899 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 501 CCAAGG C^C[AT][AT]GG
HindIII 800 AAGCTT A^AGCTT
MaeIII 514 GTAAC ^GT[ACGT]AC
MluNI 1176 TGGCCA TGG^CCA
MscI 1176 TGGCCA TGG^CCA
NspI 566 ACATGC [GA]CATG^[CT]
PaeR7I 1189 CTCGAG C^TCGAG
PflMI 1152 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 420 TCATGA T^CATGA
SapI 532 GAAGAGC GCTCTTC(1/4)
Sau96I 900 GGCCC G^G[ACGT]CC
SfcI 860 CTATAG C^T[GA][CT]AG
SmlI 1189 CTCGAG C^T[CT][GA]AG
StyI 501 CCAAGG C^C[AT][AT]GG
Van91I 1152 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 702 CCAGTCCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1189 CTCGAG C^TCGAG
XmnI 217 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 400 GTAGAC GT^[AC][GT]AC
522 GTATAC
AfaI 547 GTAC GT^AC
695 GTAC
AflIII 396 ACGTGT A^C[GA][CT]GT
642 ACGTGT
AhdI 349 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
485 GACCTGATGTC
AspEI 349 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
485 GACCTGATGTC
BbsI 482 GAAGAC GAAGAC(2/6)
1051 GAAGAC
BclI 1086 TGATCA T^GATCA
1247 TGATCA
BpmI 260 CTCCAG CTGGAG(16/14)
1124 CTGGAG
BpuAI 482 GAAGAC GAAGAC(2/6)
1051 GAAGAC
BsiYI 707 CCAGTGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1152 CCAAGAGCTGG
BslI 707 CCAGTGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1152 CCAAGAGCTGG
BsrDI 450 CATTGC GCAATG(2/0)
945 CATTGC
BstBI 268 TTCGAA TT^CGAA
336 TTCGAA
EaeI 254 TGGCCG [CT]^GGCC[GA]
1176 TGGCCA
Eam1105I 349 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
485 GACCTGATGTC
FbaI 1086 TGATCA T^GATCA
1247 TGATCA
MaeII 396 ACGT A^CGT
642 ACGT
NspV 268 TTCGAA TT^CGAA
336 TTCGAA
RsaI 547 GTAC GT^AC
695 GTAC
SfuI 268 TTCGAA TT^CGAA
336 TTCGAA
SspI 430 AATATT AAT^ATT
792 AATATT
TfiI 306 GAATC G^A[AT]TC
1269 GAATC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfaNI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII