Restriction map for
pF1KB9898
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AvrII
BbeI BbrPI BcgI BfrI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
HaeII Hin1I HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 5012 GTCTAC GT^[AC][GT]AC
AflIII 1401 ACATGT A^C[GA][CT]GT
AhdI 4993 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1978 GTGCAC G^TGCAC
ApaLI 1978 GTGCAC G^TGCAC
Asp700I 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4993 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5975 CTCGGG C^[CT]CG[GA]G
AviII 2733 TGCGCA TGC^GCA
BbsI 4201 GAAGAC GAAGAC(2/6)
BclI 2301 TGATCA T^GATCA
BcnI 5308 CCGGG CC^[GC]GG
BpuAI 4201 GAAGAC GAAGAC(2/6)
BsaAI 4490 CACGTA [CT]AC^GT[GA]
BsaBI 3681 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 5975 CTCGGG C^[CT]CG[GA]G
Bsp1407I 5142 TGTACA T^GTACA
BspHI 5178 TCATGA T^CATGA
BspLU11I 1401 ACATGT A^CATGT
BsrFI 3782 GCCGGT [GA]^CCGG[CT]
BsrGI 5142 TGTACA T^GTACA
BssSI 1646 CACGAG CACGAG(-5/-1)
BstXI 3989 CCACTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3782 GCCGGT [GA]^CCGG[CT]
Eam1105I 4993 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1255 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4759 GATATC GAT^ATC
EcoT22I 4028 ATGCAT ATGCA^T
FbaI 2301 TGATCA T^GATCA
FspI 2733 TGCGCA TGC^GCA
MamI 3681 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1207 CAATTG C^AATTG
MunI 1207 CAATTG C^AATTG
NciI 5308 CCGGG CC^[GC]GG
NdeI 5963 CATATG CA^TATG
NheI 1035 GCTAGC G^CTAGC
NsiI 4028 ATGCAT ATGCA^T
PshAI 1517 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5178 TCATGA T^CATGA
SacI 128 GAGCTC GAGCT^C
SexAI 1973 ACCAGGT A^CC[AT]GGT
SgrAI 3781 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 5493 ACTAGT A^CTAGT
SspBI 5142 TGTACA T^GTACA
XbaI 5212 TCTAGA T^CTAGA
XmnI 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BbvCI 1246 CCTCAGC CCTCAGC(-5/-2)
3753 GCTGAGG
BciVI 3360 GGATAC GTATCC(6/5)
4980 GTATCC
BglI 1833 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5755 GCCAAAATGGC
BglII 1650 AGATCT A^GATCT
2628 AGATCT
BlpI 2743 GCTCAGC GC^T[ACGT]AGC
5860 GCTGAGC
Bpu1102I 2743 GCTCAGC GC^T[ACGT]AGC
5860 GCTGAGC
BsaI 3076 GAGACC GGTCTC(1/5)
4626 GAGACC
BsmBI 3748 GAGACG CGTCTC(1/5)
4710 GAGACG
CelII 2743 GCTCAGC GC^T[ACGT]AGC
5860 GCTGAGC
CfoI 2734 GCGC GCG^C
3812 GCGC
DraI 1110 TTTAAA TTT^AAA
5655 TTTAAA
EcoRI 1051 GAATTC G^AATTC
4827 GAATTC
FauI 3708 CCCGC CCCGC(4/6)
3639 GCGGG
HapII 3783 CCGG C^CGG
5308 CCGG
HgaI 3750 GACGC GACGC(5/10)
1039 GCGTC
HhaI 2734 GCGC GCG^C
3812 GCGC
HinP1I 2734 GCGC G^CGC
3812 GCGC
HpaII 3783 CCGG C^CGG
5308 CCGG
MspI 3783 CCGG C^CGG
5308 CCGG
NcoI 3080 CCATGG C^CATGG
5832 CCATGG
PpuMI 3432 AGGTCCT [GA]G^G[AT]CC[CT]
3459 AGGTCCT
SmlI 1534 CTCAAG C^T[CT][GA]AG
4805 CTCAAG
SspI 695 AATATT AAT^ATT
2597 AATATT
StuI 881 AGGCCT AGG^CCT
1435 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AspI
AvaII BalI BamHI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII