Restriction map for
pF1KE2618
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BcgI BclI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MluI MluNI
MroI MscI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 1132 GACGTC GACGT^C
Acc65I 579 GGTACC G^GTACC
AccI 406 GTCTAC GT^[AC][GT]AC
AflIII 251 ACGTGT A^C[GA][CT]GT
AocI 376 CCTCAGG CC^T[ACGT]AGG
Asp718I 579 GGTACC G^GTACC
AvaII 78 GGACC G^G[AT]CC
BbeI 150 GGCGCC GGCGC^C
BbvCI 1000 CCTCAGC CCTCAGC(-5/-2)
BciVI 869 GTATCC GTATCC(6/5)
BfaI 500 CTAG C^TAG
BglI 277 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 626 TGTACA T^GTACA
BsrGI 626 TGTACA T^GTACA
Bsu36I 376 CCTCAGG CC^T[ACGT]AGG
EaeI 284 CGGCCA [CT]^GGCC[GA]
EarI 764 GAAGAG CTCTTC(1/4)
Eco81I 376 CCTCAGG CC^T[ACGT]AGG
EcoNI 1079 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 150 GGCGCC G^GCGCC
KpnI 579 GGTACC GGTAC^C
Ksp632I 764 GAAGAG CTCTTC(1/4)
MaeI 500 CTAG C^TAG
MfeI 1010 CAATTG C^AATTG
MseI 682 TTAA T^TAA
MslI 429 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1010 CAATTG C^AATTG
NarI 150 GGCGCC GG^CGCC
NspI 340 ACATGC [GA]CATG^[CT]
SfoI 150 GGCGCC GGC^GCC
SspBI 626 TGTACA T^GTACA
Tru9I 682 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 132 CGCG CG^CG
394 CGCG
AcsI 472 GAATTC [GA]^AATT[CT]
610 GAATTC
AcyI 150 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
ApoI 472 GAATTC [GA]^AATT[CT]
610 GAATTC
AspHI 270 GAGCAC G[AT]GC[AT]^C
421 GAGCAC
BanII 61 GGGCTC G[GA]GC[CT]^C
1102 GAGCCC
BbsI 563 GAAGAC GAAGAC(2/6)
1135 GTCTTC
BcnI 743 CCCGG CC^[GC]GG
1106 CCCGG
BlpI 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
Bpu1102I 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
BpuAI 563 GAAGAC GAAGAC(2/6)
1135 GTCTTC
BsaHI 150 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
BseRI 99 CTCCTC GAGGAG(10/8)
374 CTCCTC
BsiHKAI 270 GAGCAC G[AT]GC[AT]^C
421 GAGCAC
BsmAI 767 GAGAC GTCTC(1/5)
943 GAGAC
BstUI 132 CGCG CG^CG
394 CGCG
CelII 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
EcoRI 472 GAATTC G^AATTC
610 GAATTC
FauI 205 CCCGC CCCGC(4/6)
237 CCCGC
HaeII 150 GGCGCC [GA]GCGC^[CT]
842 AGCGCC
HapII 744 CCGG C^CGG
1107 CCGG
HgaI 728 GACGC GACGC(5/10)
143 GCGTC
Hin1I 150 GGCGCC G[GA]^CG[CT]C
1132 GACGTC
HpaII 744 CCGG C^CGG
1107 CCGG
MspI 744 CCGG C^CGG
1107 CCGG
MvnI 132 CGCG CG^CG
394 CGCG
NciI 743 CCCGG CC^[GC]GG
1106 CCCGG
PleI 27 GACTC GAGTC(4/5)
973 GAGTC
SfaNI 187 GATGC GCATC(5/9)
791 GCATC
TfiI 87 GATTC G^A[AT]TC
510 GATTC
Tsp45I 145 GTCAC ^GT[GC]AC
427 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TseI Tsp509I TspRI
TthHB8I XhoII