Restriction map for
pF1KB5708
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI MaeI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 1334 ACACGT A^C[GA][CT]GT
BalI 405 TGGCCA TGG^CCA
BciVI 2111 GTATCC GTATCC(6/5)
BclI 1035 TGATCA T^GATCA
BglI 1141 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 604 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 253 CGGTCG CG[GA][CT]^CG
BspHI 74 TCATGA T^CATGA
BsrBI 85 CCGCTC CCGCTC(-3/-3)
BssSI 1162 CACGAG CACGAG(-5/-1)
BstAPI 1459 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 431 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1179 CTCTTC CTCTTC(1/4)
FbaI 1035 TGATCA T^GATCA
HincII 1986 GTTGAC GT[CT]^[GA]AC
HindII 1986 GTTGAC GT[CT]^[GA]AC
HindIII 1014 AAGCTT A^AGCTT
Ksp632I 1179 CTCTTC CTCTTC(1/4)
KspI 30 CCGCGG CCGC^GG
MaeIII 1331 GTGAC ^GT[ACGT]AC
MamI 604 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 405 TGGCCA TGG^CCA
MscI 405 TGGCCA TGG^CCA
NdeI 298 CATATG CA^TATG
PflMI 408 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 74 TCATGA T^CATGA
SacII 30 CCGCGG CCGC^GG
ScaI 2038 AGTACT AGT^ACT
SmlI 1633 CTTGAG C^T[CT][GA]AG
TaqI 256 TCGA T^CGA
Tsp45I 1331 GTGAC ^GT[GC]AC
TthHB8I 256 TCGA T^CGA
Van91I 408 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 297 CCATATGGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 858 GTAC GT^AC
2039 GTAC
ApaI 165 GGGCCC GGGCC^C
499 GGGCCC
Asp700I 601 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1879 GAACTCATTC
AvaI 132 CCCGGG C^[CT]CG[GA]G
586 CCCGGG
BpmI 1080 CTCCAG CTGGAG(16/14)
1708 CTCCAG
BseRI 198 CTCCTC GAGGAG(10/8)
210 CTCCTC
BsgI 1486 GTGCAG GTGCAG(16/14)
1673 GTGCAG
BsoBI 132 CCCGGG C^[CT]CG[GA]G
586 CCCGGG
BstNI 846 CCAGG CC^[AT]GG
1578 CCAGG
EaeI 405 TGGCCA [CT]^GGCC[GA]
1836 TGGCCG
EcoRII 846 CCAGG ^CC[AT]GG
1578 CCAGG
MaeII 1336 ACGT A^CGT
2109 ACGT
MslI 610 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1552 CATGTGTGTG
MvaI 846 CCAGG CC^[AT]GG
1578 CCAGG
NcoI 771 CCATGG C^CATGG
1151 CCATGG
PvuII 150 CAGCTG CAG^CTG
1859 CAGCTG
RsaI 858 GTAC GT^AC
2039 GTAC
SacI 741 GAGCTC GAGCT^C
2141 GAGCTC
SmaI 132 CCCGGG CCC^GGG
586 CCCGGG
SspI 1054 AATATT AAT^ATT
1291 AATATT
XmaCI 132 CCCGGG C^CCGGG
586 CCCGGG
XmaI 132 CCCGGG C^CCGGG
586 CCCGGG
XmnI 601 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1879 GAACTCATTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MboI MboII MflI
MnlI MseI MspA1I MspI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SfaNI SfoI StyI TfiI
Tru9I TseI Tsp509I TspRI XhoII