Restriction map for
pF1KE3139
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BsaBI BsaI BsiEI BsiYI BslI
BsmFI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NcoI NdeI NheI NotI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 575 TCCGGA T^CCGGA
AcsI 985 AAATTT [GA]^AATT[CT]
AflIII 215 ACACGT A^C[GA][CT]GT
Aor51HI 599 AGCGCT AGC^GCT
ApoI 985 AAATTT [GA]^AATT[CT]
AspI 997 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 862 GGACC G^G[AT]CC
BalI 233 TGGCCA TGG^CCA
BamHI 620 GGATCC G^GATCC
BanI 106 GGTGCC G^G[CT][GA]CC
BanII 913 GAGCTC G[GA]GC[CT]^C
BciVI 392 GTATCC GTATCC(6/5)
BfaI 688 CTAG C^TAG
BglII 941 AGATCT A^GATCT
BlpI 666 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 666 GCTGAGC GC^T[ACGT]AGC
BsaAI 487 TACGTG [CT]AC^GT[GA]
BseAI 575 TCCGGA T^CCGGA
BsiWI 240 CGTACG C^GTACG
BsmBI 544 GAGACG CGTCTC(1/5)
BspEI 575 TCCGGA T^CCGGA
BspMI 643 ACCTGC ACCTGC(4/8)
BssHII 411 GCGCGC G^CGCGC
BssSI 718 CACGAG CACGAG(-5/-1)
BstAPI 501 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 474 GGTAACC G^GT[ACGT]ACC
BstPI 474 GGTAACC G^GT[ACGT]ACC
BstXI 35 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 666 GCTGAGC GC^T[ACGT]AGC
EaeI 233 TGGCCA [CT]^GGCC[GA]
Eco47III 599 AGCGCT AGC^GCT
EcoO65I 474 GGTAACC G^GT[ACGT]ACC
EcoT14I 347 CCAAGG C^C[AT][AT]GG
MaeI 688 CTAG C^TAG
MluNI 233 TGGCCA TGG^CCA
MroI 575 TCCGGA T^CCGGA
MscI 233 TGGCCA TGG^CCA
MseI 791 TTAA T^TAA
MslI 894 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 370 CAGCGG C[AC]G^C[GT]G
NaeI 883 GCCGGC GCC^GGC
NgoMIV 883 GCCGGC G^CCGGC
NruI 517 TCGCGA TCG^CGA
PflFI 997 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 913 GAGCTC GAGCT^C
SmaI 480 CCCGGG CCC^GGG
SphI 713 GCATGC GCATG^C
StuI 398 AGGCCT AGG^CCT
StyI 347 CCAAGG C^C[AT][AT]GG
Tru9I 791 TTAA T^TAA
Tsp509I 986 AATT ^AATT
Tth111I 997 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 480 CCCGGG C^CCGGG
XmaI 480 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 771 GACGTC GACGT^C
802 GACGTC
BbsI 805 GTCTTC GAAGAC(2/6)
991 GTCTTC
BbvCI 834 CCTCAGC CCTCAGC(-5/-2)
19 GCTGAGG
BpmI 33 CTCCAG CTGGAG(16/14)
463 CTGGAG
BpuAI 805 GTCTTC GAAGAC(2/6)
991 GTCTTC
BsaWI 388 TCCGGT [AT]^CCGG[AT]
575 TCCGGA
BseRI 22 GAGGAG GAGGAG(10/8)
331 GAGGAG
BsmAI 275 GAGAC GTCTC(1/5)
544 GAGAC
BsrFI 109 GCCGGT [GA]^CCGG[CT]
883 GCCGGC
Cfr10I 109 GCCGGT [GA]^CCGG[CT]
883 GCCGGC
Cfr13I 11 GGGCC G^G[ACGT]CC
862 GGACC
FauI 442 GCGGG CCCGC(4/6)
593 GCGGG
HphI 112 GGTGA GGTGA(8/7)
1004 TCACC
NspI 713 GCATGC [GA]CATG^[CT]
920 ACATGC
PaeR7I 187 CTCGAG C^TCGAG
910 CTCGAG
SapI 123 GAAGAGC GCTCTTC(1/4)
783 GCTCTTC
Sau96I 11 GGGCC G^G[ACGT]CC
862 GGACC
SmlI 187 CTCGAG C^T[CT][GA]AG
910 CTCGAG
Tsp45I 640 GTGAC ^GT[GC]AC
1003 GTCAC
XhoI 187 CTCGAG C^TCGAG
910 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiHKAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI TaqI TseI TspRI
TthHB8I XhoII