Restriction map for
pF1KB4872
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# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AcsI AflII Aor51HI ApoI
AscI AseI AsnI BfrI BsaBI
BsiWI Bsp1407I BspDI BspHI BsrGI
BstBI ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI HindIII HpaI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshBI Psp1406I RcaI
RsrII SalI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 4538 GACGTC GACGT^C
AviII 1622 TGCGCA TGC^GCA
AvrII 2808 CCTAGG C^CTAGG
BbrPI 2620 CACGTG CAC^GTG
BbvCI 2646 GCTGAGG CCTCAGC(-5/-2)
BclI 437 TGATCA T^GATCA
BglII 7167 AGATCT A^GATCT
BlnI 2808 CCTAGG C^CTAGG
BspLU11I 3431 ACATGT A^CATGT
BssHII 2848 GCGCGC G^CGCGC
Bst1107I 2244 GTATAC GTA^TAC
BstZ17I 2244 GTATAC GTA^TAC
EarI 8524 GAAGAG CTCTTC(1/4)
FbaI 437 TGATCA T^GATCA
FspI 1622 TGCGCA TGC^GCA
HincII 7760 GTCAAC GT[CT]^[GA]AC
HindII 7760 GTCAAC GT[CT]^[GA]AC
Ksp632I 8524 GAAGAG CTCTTC(1/4)
KspI 5169 CCGCGG CCGC^GG
MseI 3554 TTAA T^TAA
NaeI 8470 GCCGGC GCC^GGC
NgoMIV 8470 GCCGGC G^CCGGC
PmaCI 2620 CACGTG CAC^GTG
PmlI 2620 CACGTG CAC^GTG
SacII 5169 CCGCGG CCGC^GG
SapI 8524 GAAGAGC GCTCTTC(1/4)
SbfI 6586 CCTGCAGG CCTGCA^GG
ScaI 8552 AGTACT AGT^ACT
Sse8387I 6586 CCTGCAGG CCTGCA^GG
Tru9I 3554 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 8532 GGTACC G^GTACC
8675 GGTACC
AccIII 681 TCCGGA T^CCGGA
1908 TCCGGA
AflIII 2886 ACGTGT A^C[GA][CT]GT
3431 ACATGT
AgeI 1331 ACCGGT A^CCGGT
3838 ACCGGT
AhdI 410 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
445 GACGGGAAGTC
Alw44I 218 GTGCAC G^TGCAC
645 GTGCAC
ApaLI 218 GTGCAC G^TGCAC
645 GTGCAC
Asp700I 5242 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8206 GAAGGACTTC
Asp718I 8532 GGTACC G^GTACC
8675 GGTACC
AspEI 410 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
445 GACGGGAAGTC
AspI 382 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACTCTGTC
BcgI 173 GCAGCTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3594 GCAGCTGCGTCG
BseAI 681 TCCGGA T^CCGGA
1908 TCCGGA
BspEI 681 TCCGGA T^CCGGA
1908 TCCGGA
BsrDI 4251 CATTGC GCAATG(2/0)
6362 GCAATG
Eam1105I 410 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
445 GACGGGAAGTC
KpnI 8532 GGTACC GGTAC^C
8675 GGTACC
MroI 681 TCCGGA T^CCGGA
1908 TCCGGA
MslI 1614 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3405 CACAGCCGTG
PflFI 382 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACTCTGTC
PinAI 1331 ACCGGT A^CCGGT
3838 ACCGGT
PvuI 2903 CGATCG CGAT^CG
4209 CGATCG
Tth111I 382 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACTCTGTC
XmnI 5242 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8206 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI