Restriction map for
pF1KE3281
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AviII
BbeI BbrPI BcgI BcnI BfrI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT22I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AgeI 879 ACCGGT A^CCGGT
AlwNI 236 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 171 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1129 CCTAGG C^CTAGG
BalI 2129 TGGCCA TGG^CCA
BamHI 432 GGATCC G^GATCC
BbvCI 301 CCTCAGC CCTCAGC(-5/-2)
BglI 187 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1129 CCTAGG C^CTAGG
BlpI 1916 GCTTAGC GC^T[ACGT]AGC
BpmI 924 CTGGAG CTGGAG(16/14)
Bpu1102I 1916 GCTTAGC GC^T[ACGT]AGC
BsaAI 1424 TACGTG [CT]AC^GT[GA]
BsaI 967 GAGACC GGTCTC(1/5)
BsaWI 879 ACCGGT [AT]^CCGG[AT]
BsmFI 71 GGGAC GGGAC(10/14)
BsmI 1281 GAATGC GAATGC(1/-1)
Bsp1407I 1799 TGTACA T^GTACA
BspMI 855 GCAGGT ACCTGC(4/8)
BsrDI 1636 GCAATG GCAATG(2/0)
BsrFI 879 ACCGGT [GA]^CCGG[CT]
BsrGI 1799 TGTACA T^GTACA
BsrI 1073 CCAGT ACTGG(1/-1)
BstAPI 1078 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 612 GGTCACC G^GT[ACGT]ACC
BstPI 612 GGTCACC G^GT[ACGT]ACC
CelII 1916 GCTTAGC GC^T[ACGT]AGC
CfoI 822 GCGC GCG^C
Cfr10I 879 ACCGGT [GA]^CCGG[CT]
DraI 1366 TTTAAA TTT^AAA
DraII 959 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 547 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2129 TGGCCA [CT]^GGCC[GA]
EcoO109I 959 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 612 GGTCACC G^GT[ACGT]ACC
EcoRV 1429 GATATC GAT^ATC
HapII 880 CCGG C^CGG
HhaI 822 GCGC GCG^C
HinP1I 822 GCGC G^CGC
HincII 556 GTCAAC GT[CT]^[GA]AC
HindII 556 GTCAAC GT[CT]^[GA]AC
HpaII 880 CCGG C^CGG
MluNI 2129 TGGCCA TGG^CCA
MscI 2129 TGGCCA TGG^CCA
MspI 880 CCGG C^CGG
PflFI 171 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 879 ACCGGT A^CCGGT
PleI 350 GACTC GAGTC(4/5)
PpuMI 959 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1909 CAGCTG CAG^CTG
SacI 209 GAGCTC GAGCT^C
SexAI 1217 ACCAGGT A^CC[AT]GGT
SfaNI 998 GCATC GCATC(5/9)
SspBI 1799 TGTACA T^GTACA
Tsp45I 613 GTCAC ^GT[GC]AC
Tth111I 171 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 456 CCATAGCTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AseI 1257 ATTAAT AT^TAAT
1516 ATTAAT
AsnI 1257 ATTAAT AT^TAAT
1516 ATTAAT
AspHI 209 GAGCTC G[AT]GC[AT]^C
1195 GTGCTC
AvaI 212 CTCGGG C^[CT]CG[GA]G
659 CCCGAG
AvaII 882 GGTCC G^G[AT]CC
960 GGACC
BanI 98 GGTGCC G^G[CT][GA]CC
655 GGTGCC
BbsI 24 GAAGAC GAAGAC(2/6)
1117 GAAGAC
BciVI 875 GGATAC GTATCC(6/5)
1453 GTATCC
BclI 6 TGATCA T^GATCA
1047 TGATCA
BpuAI 24 GAAGAC GAAGAC(2/6)
1117 GAAGAC
BsgI 833 GTGCAG GTGCAG(16/14)
2092 GTGCAG
BsiHKAI 209 GAGCTC G[AT]GC[AT]^C
1195 GTGCTC
BsoBI 212 CTCGGG C^[CT]CG[GA]G
659 CCCGAG
Cfr13I 882 GGTCC G^G[ACGT]CC
960 GGACC
DraIII 538 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1216 CACCAGGTG
EcoT14I 335 CCTTGG C^C[AT][AT]GG
1129 CCTAGG
FbaI 6 TGATCA T^GATCA
1047 TGATCA
MspA1I 809 CAGCGG C[AC]G^C[GT]G
1909 CAGCTG
PshBI 1257 ATTAAT AT^TAAT
1516 ATTAAT
PstI 587 CTGCAG CTGCA^G
1085 CTGCAG
SapI 529 GAAGAGC GCTCTTC(1/4)
1921 GCTCTTC
Sau96I 882 GGTCC G^G[ACGT]CC
960 GGACC
SfcI 587 CTGCAG C^T[GA][CT]AG
1085 CTGCAG
SmlI 1122 CTTGAG C^T[CT][GA]AG
1326 CTTGAG
StyI 335 CCTTGG C^C[AT][AT]GG
1129 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BfaI BglII
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI ScrFI SspI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII