Restriction map for
pF1KB6651
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaII KasI
KpnI Ksp632I KspI MamI MluI
MroI MslI MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 390 GAATTT [GA]^AATT[CT]
AfaI 909 GTAC GT^AC
AlwI 1103 GGATC GGATC(4/5)
AocI 490 CCTGAGG CC^T[ACGT]AGG
ApoI 390 GAATTT [GA]^AATT[CT]
AspHI 936 GAGCTC G[AT]GC[AT]^C
BalI 326 TGGCCA TGG^CCA
BbsI 418 GAAGAC GAAGAC(2/6)
BbvCI 609 CCTCAGC CCTCAGC(-5/-2)
BfaI 187 CTAG C^TAG
BglII 1149 AGATCT A^GATCT
BpuAI 418 GAAGAC GAAGAC(2/6)
BsiHKAI 936 GAGCTC G[AT]GC[AT]^C
BsrDI 802 CATTGC GCAATG(2/0)
BstYI 1149 AGATCT [GA]^GATC[CT]
Bsu36I 490 CCTGAGG CC^T[ACGT]AGG
CfoI 13 GCGC GCG^C
DraII 673 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 326 TGGCCA [CT]^GGCC[GA]
Eco81I 490 CCTGAGG CC^T[ACGT]AGG
EcoO109I 673 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 875 ATGCAT ATGCA^T
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
HincII 643 GTTAAC GT[CT]^[GA]AC
HindII 643 GTTAAC GT[CT]^[GA]AC
HpaI 643 GTTAAC GTT^AAC
HphI 107 TCACC GGTGA(8/7)
MaeI 187 CTAG C^TAG
MaeII 432 ACGT A^CGT
MfeI 383 CAATTG C^AATTG
MflI 1149 AGATCT [GA]^GATC[CT]
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
MunI 383 CAATTG C^AATTG
NsiI 875 ATGCAT ATGCA^T
PflMI 329 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 304 CTGCAG CTGCA^G
RsaI 909 GTAC GT^AC
SacI 936 GAGCTC GAGCT^C
SfaNI 484 GATGC GCATC(5/9)
SphI 583 GCATGC GCATG^C
Van91I 329 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 186 TCTAGA T^CTAGA
XhoII 1149 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 294 GCGG CCGC(-3/-1)
634 GCGG
AlwNI 330 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
782 CAGTGTCTG
AvaII 296 GGACC G^G[AT]CC
1184 GGTCC
BanII 936 GAGCTC G[GA]GC[CT]^C
958 GGGCTC
BbvI 332 GCAGC GCAGC(8/12)
928 GCAGC
BmyI 936 GAGCTC G[AGT]GC[ACT]^C
958 GGGCTC
BsaI 1237 GAGACC GGTCTC(1/5)
368 GGTCTC
BsgI 462 CTGCAC GTGCAG(16/14)
945 GTGCAG
BsmBI 757 CGTCTC CGTCTC(1/5)
1192 GAGACG
BsmI 541 GAATGC GAATGC(1/-1)
877 GCATTC
Bsp1286I 936 GAGCTC G[AGT]GC[ACT]^C
958 GGGCTC
Fnu4HI 332 GCAGC GC^[ACGT]GC
928 GCAGC
HinfI 239 GAATC G^A[ACGT]TC
1249 GATTC
ItaI 332 GCAGC GC^[ACGT]GC
928 GCAGC
MspA1I 333 CAGCTG C[AC]G^C[GT]G
685 CAGCTG
NspI 15 GCATGT [GA]CATG^[CT]
583 GCATGC
PvuII 333 CAGCTG CAG^CTG
685 CAGCTG
SmlI 131 CTCAAG C^T[CT][GA]AG
1014 CTTGAG
TfiI 239 GAATC G^A[AT]TC
1249 GATTC
TseI 332 GCAGC G^C[AT]GC
928 GCAGC
Tsp45I 623 GTGAC ^GT[GC]AC
733 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BclI BpmI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FbaI FokI HaeIII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfcI Tru9I Tsp509I
TspRI