Restriction map for
pF1KE5665
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspMI
BsrGI BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AocI 33 CCTGAGG CC^T[ACGT]AGG
ApaI 7 GGGCCC GGGCC^C
AvaI 1245 CCCGGG C^[CT]CG[GA]G
BalI 263 TGGCCA TGG^CCA
BamHI 215 GGATCC G^GATCC
BbeI 1485 GGCGCC GGCGC^C
BlpI 951 GCTGAGC GC^T[ACGT]AGC
BpmI 1459 CTGGAG CTGGAG(16/14)
Bpu1102I 951 GCTGAGC GC^T[ACGT]AGC
BsaAI 1252 TACGTG [CT]AC^GT[GA]
BsiEI 993 CGGCCG CG[GA][CT]^CG
BsmBI 253 GAGACG CGTCTC(1/5)
BsoBI 1245 CCCGGG C^[CT]CG[GA]G
BspHI 1014 TCATGA T^CATGA
BspLU11I 674 ACATGT A^CATGT
BsrBI 1296 GAGCGG CCGCTC(-3/-3)
BsrFI 526 GCCGGC [GA]^CCGG[CT]
BssHII 1378 GCGCGC G^CGCGC
BstEII 492 GGTGACC G^GT[ACGT]ACC
BstPI 492 GGTGACC G^GT[ACGT]ACC
Bsu36I 33 CCTGAGG CC^T[ACGT]AGG
CelII 951 GCTGAGC GC^T[ACGT]AGC
Cfr10I 526 GCCGGC [GA]^CCGG[CT]
EagI 993 CGGCCG C^GGCCG
EarI 169 CTCTTC CTCTTC(1/4)
EclXI 993 CGGCCG C^GGCCG
Eco52I 993 CGGCCG C^GGCCG
Eco81I 33 CCTGAGG CC^T[ACGT]AGG
EcoNI 753 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 492 GGTGACC G^GT[ACGT]ACC
HaeII 1485 GGCGCC [GA]GCGC^[CT]
HinfI 731 GAATC G^A[ACGT]TC
KasI 1485 GGCGCC G^GCGCC
Ksp632I 169 CTCTTC CTCTTC(1/4)
KspI 966 CCGCGG CCGC^GG
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
MseI 410 TTAA T^TAA
NaeI 526 GCCGGC GCC^GGC
NarI 1485 GGCGCC GG^CGCC
NgoMIV 526 GCCGGC G^CCGGC
NotI 992 GCGGCCGC GC^GGCCGC
PflMI 846 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 133 CTGCAG CTGCA^G
RcaI 1014 TCATGA T^CATGA
SacII 966 CCGCGG CCGC^GG
SfoI 1485 GGCGCC GGC^GCC
SmaI 1245 CCCGGG CCC^GGG
SmlI 390 CTTGAG C^T[CT][GA]AG
TfiI 731 GAATC G^A[AT]TC
Tru9I 410 TTAA T^TAA
Van91I 846 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1175 CCATCTTCGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1245 CCCGGG C^CCGGG
XmaI 1245 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 811 GAGCTC G[AT]GC[AT]^C
1170 GAGCTC
BbsI 1360 GAAGAC GAAGAC(2/6)
1138 GTCTTC
BpuAI 1360 GAAGAC GAAGAC(2/6)
1138 GTCTTC
BsaI 896 GAGACC GGTCTC(1/5)
1345 GAGACC
BsiHKAI 811 GAGCTC G[AT]GC[AT]^C
1170 GAGCTC
BsrDI 434 GCAATG GCAATG(2/0)
1238 GCAATG
BstAPI 1395 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1404 GCATGGTGTGC
BstYI 215 GGATCC [GA]^GATC[CT]
887 AGATCC
DraIII 541 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1396 CACCTGGTG
MflI 215 GGATCC [GA]^GATC[CT]
887 AGATCC
PpuMI 688 GGGACCC [GA]G^G[AT]CC[CT]
1549 GGGACCC
PshAI 1003 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1110 GACCCTCGTC
SacI 811 GAGCTC GAGCT^C
1170 GAGCTC
SexAI 752 ACCTGGT A^CC[AT]GGT
1397 ACCTGGT
SfcI 40 CTACAG C^T[GA][CT]AG
133 CTGCAG
TaqI 1001 TCGA T^CGA
1058 TCGA
TthHB8I 1001 TCGA T^CGA
1058 TCGA
XhoII 215 GGATCC [GA]^GATC[CT]
887 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI