Restriction map for
pF1KE2455
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 481 AAATTC [GA]^AATT[CT]
AocI 1168 CCTCAGG CC^T[ACGT]AGG
ApoI 481 AAATTC [GA]^AATT[CT]
AvaII 1380 GGACC G^G[AT]CC
BbvCI 587 CCTCAGC CCTCAGC(-5/-2)
BglI 757 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 925 GAGACC GGTCTC(1/5)
BsgI 261 GTGCAG GTGCAG(16/14)
BsiEI 37 CGGCCG CG[GA][CT]^CG
BsmBI 246 CGTCTC CGTCTC(1/5)
Bsp1407I 1014 TGTACA T^GTACA
BsrDI 1154 GCAATG GCAATG(2/0)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BsrGI 1014 TGTACA T^GTACA
BstXI 270 CCAGGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1168 CCTCAGG CC^T[ACGT]AGG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco52I 37 CGGCCG C^GGCCG
Eco81I 1168 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1426 CCTTGG C^C[AT][AT]GG
HaeII 16 GGCGCT [GA]GCGC^[CT]
HincII 112 GTCAAC GT[CT]^[GA]AC
HindII 112 GTCAAC GT[CT]^[GA]AC
NaeI 13 GCCGGC GCC^GGC
NdeI 1303 CATATG CA^TATG
NgoMIV 13 GCCGGC G^CCGGC
NspI 380 GCATGT [GA]CATG^[CT]
PstI 1284 CTGCAG CTGCA^G
SapI 1308 GAAGAGC GCTCTTC(1/4)
SmlI 412 CTCAAG C^T[CT][GA]AG
SspBI 1014 TGTACA T^GTACA
StyI 1426 CCTTGG C^C[AT][AT]GG
TfiI 1029 GATTC G^A[AT]TC
XcmI 839 CCAATCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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Acc65I 266 GGTACC G^GTACC
1203 GGTACC
AlwI 255 GATCC GGATC(4/5)
967 GATCC
ApaI 42 GGGCCC GGGCC^C
1123 GGGCCC
Asp718I 266 GGTACC G^GTACC
1203 GGTACC
AspI 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
BanII 42 GGGCCC G[GA]GC[CT]^C
1123 GGGCCC
BfaI 743 CTAG C^TAG
1297 CTAG
BpmI 691 CTGGAG CTGGAG(16/14)
700 CTGGAG
BseRI 499 GAGGAG GAGGAG(10/8)
502 GAGGAG
BsmFI 1179 GGGAC GGGAC(10/14)
234 GTCCC
BspMI 263 GCAGGT ACCTGC(4/8)
321 GCAGGT
BstYI 254 AGATCC [GA]^GATC[CT]
966 AGATCC
DraII 1122 GGGGCCC [GA]G^G[ACGT]CC[CT]
1123 GGGCCCT
EarI 496 GAAGAG CTCTTC(1/4)
1308 GAAGAG
EcoNI 96 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1384 CCTGTCACAGG
EcoO109I 1122 GGGGCCC [GA]G^G[ACGT]CC[CT]
1123 GGGCCCT
KpnI 266 GGTACC GGTAC^C
1203 GGTACC
Ksp632I 496 GAAGAG CTCTTC(1/4)
1308 GAAGAG
MaeI 743 CTAG C^TAG
1297 CTAG
MaeIII 325 GTGAC ^GT[ACGT]AC
1387 GTCAC
MflI 254 AGATCC [GA]^GATC[CT]
966 AGATCC
MslI 888 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1266 CACCACGGTG
PflFI 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
PleI 622 GACTC GAGTC(4/5)
1181 GACTC
TaqI 432 TCGA T^CGA
563 TCGA
Tsp45I 325 GTGAC ^GT[GC]AC
1387 GTCAC
Tth111I 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
TthHB8I 432 TCGA T^CGA
563 TCGA
XhoII 254 AGATCC [GA]^GATC[CT]
966 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TseI Tsp509I TspRI XmaCI
XmaI