Restriction map for
pF1KE3341
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII AclI AflII AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbeI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsiEI Bsp1407I
BspDI BspMI BsrGI BssHII BstBI
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HaeII HpaI KasI
KspI MluI MluNI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PmeI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 762 GGTACC G^GTACC
AccI 73 GTATAC GT^[AC][GT]AC
AccII 81 CGCG CG^CG
AccIII 2322 TCCGGA T^CCGGA
AflIII 2627 ACATGT A^C[GA][CT]GT
AgeI 2777 ACCGGT A^CCGGT
AocI 2130 CCTCAGG CC^T[ACGT]AGG
Asp718I 762 GGTACC G^GTACC
AspI 52 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2709 GGATCC G^GATCC
BanI 762 GGTACC G^G[CT][GA]CC
BclI 2087 TGATCA T^GATCA
BsaBI 2355 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1650 GGTCTC GGTCTC(1/5)
BseAI 2322 TCCGGA T^CCGGA
BsgI 972 CTGCAC GTGCAG(16/14)
BspEI 2322 TCCGGA T^CCGGA
BspHI 488 TCATGA T^CATGA
BspLU11I 2627 ACATGT A^CATGT
BsrFI 2777 ACCGGT [GA]^CCGG[CT]
BssSI 915 CTCGTG CACGAG(-5/-1)
Bst1107I 73 GTATAC GTA^TAC
BstAPI 2928 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 81 CGCG CG^CG
BstZ17I 73 GTATAC GTA^TAC
Bsu36I 2130 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2777 ACCGGT [GA]^CCGG[CT]
DraIII 912 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 854 CCACGG C^C[GA][CT]GG
Eco81I 2130 CCTCAGG CC^T[ACGT]AGG
EcoRI 2725 GAATTC G^AATTC
EcoRV 1495 GATATC GAT^ATC
EcoT22I 2123 ATGCAT ATGCA^T
FauI 79 CCCGC CCCGC(4/6)
FbaI 2087 TGATCA T^GATCA
HindIII 821 AAGCTT A^AGCTT
KpnI 762 GGTACC GGTAC^C
MamI 2355 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2384 CAATTG C^AATTG
MroI 2322 TCCGGA T^CCGGA
MunI 2384 CAATTG C^AATTG
MvnI 81 CGCG CG^CG
NdeI 1784 CATATG CA^TATG
NsiI 2123 ATGCAT ATGCA^T
PflFI 52 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2777 ACCGGT A^CCGGT
PleI 1959 GACTC GAGTC(4/5)
PpuMI 2846 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 488 TCATGA T^CATGA
SacI 2682 GAGCTC GAGCT^C
SnaBI 1800 TACGTA TAC^GTA
SphI 2174 GCATGC GCATG^C
TaqI 687 TCGA T^CGA
Tth111I 52 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 687 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 337 GGCGTC G[GA]^CG[CT]C
1913 GGCGTC
Alw44I 918 GTGCAC G^TGCAC
2830 GTGCAC
ApaLI 918 GTGCAC G^TGCAC
2830 GTGCAC
BbrPI 1534 CACGTG CAC^GTG
2833 CACGTG
BglII 109 AGATCT A^GATCT
1940 AGATCT
BpmI 2685 CTCCAG CTGGAG(16/14)
2758 CTCCAG
BsaHI 337 GGCGTC G[GA]^CG[CT]C
1913 GGCGTC
BsiWI 1802 CGTACG C^GTACG
2456 CGTACG
BsmI 814 GAATGC GAATGC(1/-1)
2125 GCATTC
BsrBI 802 GAGCGG CCGCTC(-3/-3)
807 GAGCGG
BstEII 1114 GGTGACC G^GT[ACGT]ACC
2525 GGTTACC
BstPI 1114 GGTGACC G^GT[ACGT]ACC
2525 GGTTACC
CfoI 2374 GCGC GCG^C
2554 GCGC
EaeI 1065 TGGCCG [CT]^GGCC[GA]
2240 TGGCCG
EcoNI 145 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2001 CCTGAGTGAGG
EcoO65I 1114 GGTGACC G^GT[ACGT]ACC
2525 GGTTACC
EcoT14I 1580 CCTTGG C^C[AT][AT]GG
2117 CCTTGG
HhaI 2374 GCGC GCG^C
2554 GCGC
Hin1I 337 GGCGTC G[GA]^CG[CT]C
1913 GGCGTC
HinP1I 2374 GCGC G^CGC
2554 GCGC
PmaCI 1534 CACGTG CAC^GTG
2833 CACGTG
PmlI 1534 CACGTG CAC^GTG
2833 CACGTG
SmlI 312 CTTGAG C^T[CT][GA]AG
2311 CTTGAG
StuI 1503 AGGCCT AGG^CCT
2602 AGGCCT
StyI 1580 CCTTGG C^C[AT][AT]GG
2117 CCTTGG
XcmI 465 CCAAGTGGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1637 CCACAGTCCTTCTGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII