Restriction map for
pF1KE2310
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AccIII AcyI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AvrII BbeI BbrPI
BbsI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BspLU11I BsrBI BssHII BstBI
CelII CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoRV FseI HaeII
HgaI Hin1I HpaI KasI KspI
MluI MroI NarI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI StuI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 2211 GGTACC G^GTACC
AccII 2777 CGCG CG^CG
AclI 2810 AACGTT AA^CGTT
AflIII 257 ACGTGT A^C[GA][CT]GT
Asp700I 453 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2211 GGTACC G^GTACC
AspI 1712 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2933 CTCGAG C^[CT]CG[GA]G
AviII 146 TGCGCA TGC^GCA
BalI 1219 TGGCCA TGG^CCA
BamHI 3818 GGATCC G^GATCC
BclI 2309 TGATCA T^GATCA
BsaAI 256 TACGTG [CT]AC^GT[GA]
BsaBI 1825 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 859 GCATTC GAATGC(1/-1)
BsoBI 2933 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1729 TGTACA T^GTACA
BspDI 338 ATCGAT AT^CGAT
BspHI 1890 TCATGA T^CATGA
BsrDI 610 CATTGC GCAATG(2/0)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BsrGI 1729 TGTACA T^GTACA
Bst1107I 2672 GTATAC GTA^TAC
BstAPI 3280 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1260 GGTGACC G^GT[ACGT]ACC
BstPI 1260 GGTGACC G^GT[ACGT]ACC
BstUI 2777 CGCG CG^CG
BstZ17I 2672 GTATAC GTA^TAC
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
ClaI 338 ATCGAT AT^CGAT
DraIII 3281 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 525 GACTCTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1260 GGTGACC G^GT[ACGT]ACC
EcoRI 2768 GAATTC G^AATTC
EcoT22I 3569 ATGCAT ATGCA^T
FauI 49 GCGGG CCCGC(4/6)
FbaI 2309 TGATCA T^GATCA
FspI 146 TGCGCA TGC^GCA
HincII 534 GTCAAC GT[CT]^[GA]AC
HindII 534 GTCAAC GT[CT]^[GA]AC
KpnI 2211 GGTACC GGTAC^C
MamI 1825 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1139 CAATTG C^AATTG
MluNI 1219 TGGCCA TGG^CCA
MscI 1219 TGGCCA TGG^CCA
MunI 1139 CAATTG C^AATTG
MvnI 2777 CGCG CG^CG
NaeI 6 GCCGGC GCC^GGC
NdeI 851 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NheI 2589 GCTAGC G^CTAGC
NsiI 3569 ATGCAT ATGCA^T
PaeR7I 2933 CTCGAG C^TCGAG
PflFI 1712 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1552 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2810 AACGTT AA^CGTT
PstI 3775 CTGCAG CTGCA^G
RcaI 1890 TCATGA T^CATGA
SacI 108 GAGCTC GAGCT^C
SbfI 3774 CCTGCAGG CCTGCA^GG
SexAI 1256 ACCTGGT A^CC[AT]GGT
SpeI 1885 ACTAGT A^CTAGT
Sse8387I 3774 CCTGCAGG CCTGCA^GG
SspBI 1729 TGTACA T^GTACA
Tth111I 1712 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2933 CTCGAG C^TCGAG
XmnI 453 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 721 CCTAAGG CC^T[ACGT]AGG
3073 CCTCAGG
BbvCI 2818 CCTCAGC CCTCAGC(-5/-2)
3055 GCTGAGG
BsaI 2936 GAGACC GGTCTC(1/5)
3533 GAGACC
BsaWI 2342 TCCGGT [AT]^CCGG[AT]
3908 ACCGGA
BspMI 287 ACCTGC ACCTGC(4/8)
3773 ACCTGC
BstYI 1589 GGATCT [GA]^GATC[CT]
3818 GGATCC
Bsu36I 721 CCTAAGG CC^T[ACGT]AGG
3073 CCTCAGG
CfoI 147 GCGC GCG^C
1361 GCGC
DraI 360 TTTAAA TTT^AAA
1747 TTTAAA
EaeI 582 TGGCCG [CT]^GGCC[GA]
1219 TGGCCA
Eco81I 721 CCTAAGG CC^T[ACGT]AGG
3073 CCTCAGG
HhaI 147 GCGC GCG^C
1361 GCGC
HinP1I 147 GCGC G^CGC
1361 GCGC
HindIII 995 AAGCTT A^AGCTT
2980 AAGCTT
MflI 1589 GGATCT [GA]^GATC[CT]
3818 GGATCC
MspA1I 1961 CAGCTG C[AC]G^C[GT]G
2377 CAGCTG
PflMI 479 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCATTAACTGG
PvuII 1961 CAGCTG CAG^CTG
2377 CAGCTG
SapI 2031 GAAGAGC GCTCTTC(1/4)
1980 GCTCTTC
SspI 354 AATATT AAT^ATT
3794 AATATT
Van91I 479 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCATTAACTGG
XcmI 812 CCACCTTTCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2276 CCAAGGCAAATGTGG
XhoII 1589 GGATCT [GA]^GATC[CT]
3818 GGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AspEI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII DsaI
Eam1105I EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I