Restriction map for
pF1KE1716
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 637 CGCG CG^CG
AcsI 300 GAATTT [GA]^AATT[CT]
AfaI 93 GTAC GT^AC
ApaI 598 GGGCCC GGGCC^C
ApoI 300 GAATTT [GA]^AATT[CT]
AvaI 595 CCCGGG C^[CT]CG[GA]G
AvaII 442 GGACC G^G[AT]CC
BanII 598 GGGCCC G[GA]GC[CT]^C
BciVI 351 GTATCC GTATCC(6/5)
BmyI 598 GGGCCC G[AGT]GC[ACT]^C
BpmI 295 CTCCAG CTGGAG(16/14)
BsgI 559 CTGCAC GTGCAG(16/14)
BsiWI 92 CGTACG C^GTACG
BsmFI 428 GTCCC GGGAC(10/14)
BsoBI 595 CCCGGG C^[CT]CG[GA]G
Bsp1286I 598 GGGCCC G[AGT]GC[ACT]^C
BsrI 174 ACTGG ACTGG(1/-1)
BstF5I 158 CATCC GGATG(2/0)
BstNI 486 CCTGG CC^[AT]GG
BstUI 637 CGCG CG^CG
DrdI 97 GACAATGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 486 CCTGG ^CC[AT]GG
FokI 158 CATCC GGATG(9/13)
HpaI 310 GTTAAC GTT^AAC
MseI 311 TTAA T^TAA
MslI 573 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 486 CCTGG CC^[AT]GG
MvnI 637 CGCG CG^CG
RsaI 93 GTAC GT^AC
SapI 378 GAAGAGC GCTCTTC(1/4)
SfaNI 157 GCATC GCATC(5/9)
SfcI 495 CTATAG C^T[GA][CT]AG
SmaI 595 CCCGGG CCC^GGG
SmlI 141 CTCAAG C^T[CT][GA]AG
TfiI 277 GAATC G^A[AT]TC
Tru9I 311 TTAA T^TAA
XmaCI 595 CCCGGG C^CCGGG
XmaI 595 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BfaI 367 CTAG C^TAG
505 CTAG
BsaJI 594 CCCCGG C^C[ACGT][ACGT]GG
595 CCCGGG
BsrDI 272 GCAATG GCAATG(2/0)
383 GCAATG
EarI 136 CTCTTC CTCTTC(1/4)
378 GAAGAG
FauI 587 CCCGC CCCGC(4/6)
605 CCCGC
HaeIII 516 GGCC GG^CC
599 GGCC
HincII 106 GTCAAC GT[CT]^[GA]AC
310 GTTAAC
HindII 106 GTCAAC GT[CT]^[GA]AC
310 GTTAAC
HphI 66 GGTGA GGTGA(8/7)
534 GGTGA
Ksp632I 136 CTCTTC CTCTTC(1/4)
378 GAAGAG
MaeI 367 CTAG C^TAG
505 CTAG
MboII 378 GAAGA GAAGA(8/7)
137 TCTTC
MwoI 3 GCTCAGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
612 GCATTACCAGC
PleI 582 GACTC GAGTC(4/5)
482 GAGTC
Tsp45I 283 GTGAC ^GT[GC]AC
566 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BcnI BseRI
BssKI Cac8I Cfr13I DdeI Fnu4HI
HapII HinfI HpaII ItaI MaeIII
MnlI MspI NciI NlaIII NlaIV
Sau96I ScrFI TseI Tsp509I TspRI