Restriction map for
pF1KE4105
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MluI MroI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Asp700I 782 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1303 GGACC G^G[AT]CC
BalI 1250 TGGCCA TGG^CCA
BanII 1378 GAGCTC G[GA]GC[CT]^C
BbvI 1083 GCTGC GCAGC(8/12)
BcnI 1077 CCGGG CC^[GC]GG
BglII 1403 AGATCT A^GATCT
BsaWI 1054 ACCGGA [AT]^CCGG[AT]
BsgI 847 CTGCAC GTGCAG(16/14)
Bsp1407I 1182 TGTACA T^GTACA
BsrBI 1093 CCGCTC CCGCTC(-3/-3)
BsrDI 897 GCAATG GCAATG(2/0)
BsrGI 1182 TGTACA T^GTACA
BstF5I 1041 CATCC GGATG(2/0)
Cac8I 1317 GCGAGC GC[ACGT]^[ACGT]GC
DraIII 850 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1250 TGGCCA [CT]^GGCC[GA]
EcoRI 42 GAATTC G^AATTC
EcoT22I 900 ATGCAT ATGCA^T
FauI 50 CCCGC CCCGC(4/6)
FokI 1041 CATCC GGATG(9/13)
HincII 994 GTCAAC GT[CT]^[GA]AC
HindII 994 GTCAAC GT[CT]^[GA]AC
HindIII 1284 AAGCTT A^AGCTT
MfeI 560 CAATTG C^AATTG
MluNI 1250 TGGCCA TGG^CCA
MscI 1250 TGGCCA TGG^CCA
MslI 715 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 560 CAATTG C^AATTG
NciI 1077 CCGGG CC^[GC]GG
NlaIV 539 GGTTCC GG[ACGT]^[ACGT]CC
NsiI 900 ATGCAT ATGCA^T
SacI 1378 GAGCTC GAGCT^C
SapI 1095 GCTCTTC GCTCTTC(1/4)
SfaNI 714 GCATC GCATC(5/9)
SfcI 505 CTACAG C^T[GA][CT]AG
SspBI 1182 TGTACA T^GTACA
TseI 1083 GCTGC G^C[AT]GC
XbaI 927 TCTAGA T^CTAGA
XcmI 1277 CCACGCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 782 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 306 GTCTAC GT^[AC][GT]AC
1357 GTCTAC
AfaI 1183 GTAC GT^AC
1395 GTAC
AlwNI 62 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
367 CAGGCTCTG
BbsI 782 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BlpI 111 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
Bpu1102I 111 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
BpuAI 782 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BsiYI 140 CCGATGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1029 CCAGTGGGAGG
BslI 140 CCGATGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1029 CCAGTGGGAGG
BstNI 314 CCTGG CC^[AT]GG
603 CCTGG
BstYI 625 AGATCC [GA]^GATC[CT]
1403 AGATCT
CelII 111 GCTCAGC GC^T[ACGT]AGC
1375 GCTGAGC
Cfr13I 437 GGGCC G^G[ACGT]CC
1303 GGACC
DsaI 29 CCGTGG C^C[GA][CT]GG
883 CCGTGG
EarI 1096 CTCTTC CTCTTC(1/4)
1003 GAAGAG
EcoRII 314 CCTGG ^CC[AT]GG
603 CCTGG
EcoT14I 54 CCAAGG C^C[AT][AT]GG
440 CCAAGG
Fnu4HI 1083 GCTGC GC^[ACGT]GC
1092 GCCGC
HaeIII 438 GGCC GG^CC
1251 GGCC
HapII 1055 CCGG C^CGG
1077 CCGG
HpaII 1055 CCGG C^CGG
1077 CCGG
HphI 137 TCACC GGTGA(8/7)
1052 TCACC
ItaI 1083 GCTGC GC^[ACGT]GC
1092 GCCGC
Ksp632I 1096 CTCTTC CTCTTC(1/4)
1003 GAAGAG
MaeII 864 ACGT A^CGT
1046 ACGT
MflI 625 AGATCC [GA]^GATC[CT]
1403 AGATCT
MspI 1055 CCGG C^CGG
1077 CCGG
MvaI 314 CCTGG CC^[AT]GG
603 CCTGG
RsaI 1183 GTAC GT^AC
1395 GTAC
Sau96I 437 GGGCC G^G[ACGT]CC
1303 GGACC
StyI 54 CCAAGG C^C[AT][AT]GG
440 CCAAGG
XhoII 625 AGATCC [GA]^GATC[CT]
1403 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BfaI BmyI BsaJI BsiHKAI
Bsp1286I BsrI BssKI DdeI DpnII
HinfI MaeI MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
Sau3AI ScrFI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I