Restriction map for
pF1KE2334
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII AclI AflII AgeI AhdI
Aor51HI ApaI AscI AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaAI BsiEI
BsiWI BspDI BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FseI FspI HaeII HpaI
KasI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 277 GGTACC G^GTACC
AccI 1351 GTAGAC GT^[AC][GT]AC
AccIII 1358 TCCGGA T^CCGGA
AcyI 4441 GACGCC G[GA]^CG[CT]C
AflIII 1761 ACATGT A^C[GA][CT]GT
Alw44I 4244 GTGCAC G^TGCAC
ApaLI 4244 GTGCAC G^TGCAC
AseI 3766 ATTAAT AT^TAAT
AsnI 3766 ATTAAT AT^TAAT
Asp718I 277 GGTACC G^GTACC
AspI 2980 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1253 CTCGGG C^[CT]CG[GA]G
BamHI 3488 GGATCC G^GATCC
BlpI 3031 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3031 GCTCAGC GC^T[ACGT]AGC
BsaHI 4441 GACGCC G[GA]^CG[CT]C
BsaWI 1358 TCCGGA [AT]^CCGG[AT]
BseAI 1358 TCCGGA T^CCGGA
BsmBI 14 GAGACG CGTCTC(1/5)
BsmI 1913 GAATGC GAATGC(1/-1)
BsoBI 1253 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4607 TGTACA T^GTACA
BspEI 1358 TCCGGA T^CCGGA
BspLU11I 1761 ACATGT A^CATGT
BsrFI 48 GCCGGT [GA]^CCGG[CT]
BsrGI 4607 TGTACA T^GTACA
BstAPI 1339 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3031 GCTCAGC GC^T[ACGT]AGC
Cfr10I 48 GCCGGT [GA]^CCGG[CT]
EcoNI 1919 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 554 GATATC GAT^ATC
EcoT22I 1966 ATGCAT ATGCA^T
FauI 37 GCGGG CCCGC(4/6)
Hin1I 4441 GACGCC G[GA]^CG[CT]C
HindIII 3102 AAGCTT A^AGCTT
KpnI 277 GGTACC GGTAC^C
KspI 35 CCGCGG CCGC^GG
MroI 1358 TCCGGA T^CCGGA
NdeI 3263 CATATG CA^TATG
NsiI 1966 ATGCAT ATGCA^T
PflFI 2980 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1677 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3766 ATTAAT AT^TAAT
SacII 35 CCGCGG CCGC^GG
SpeI 4044 ACTAGT A^CTAGT
SspBI 4607 TGTACA T^GTACA
StuI 1290 AGGCCT AGG^CCT
Tth111I 2980 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1677 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2278 CCAGTAACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 18 CGCG CG^CG
36 CGCG
AocI 2771 CCTCAGG CC^T[ACGT]AGG
3398 CCTTAGG
BanII 1095 GAGCTC G[GA]GC[CT]^C
2719 GAGCTC
BglI 3539 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3963 GCCCCGGTGGC
BsaI 1522 GAGACC GGTCTC(1/5)
1981 GAGACC
BseRI 111 CTCCTC GAGGAG(10/8)
2746 CTCCTC
BsgI 877 CTGCAC GTGCAG(16/14)
1514 CTGCAC
BsrBI 21 GAGCGG CCGCTC(-3/-3)
2003 GAGCGG
BstUI 18 CGCG CG^CG
36 CGCG
Bsu36I 2771 CCTCAGG CC^T[ACGT]AGG
3398 CCTTAGG
CfoI 43 GCGC GCG^C
2866 GCGC
DsaI 35 CCGCGG C^C[GA][CT]GG
3379 CCACGG
Eco81I 2771 CCTCAGG CC^T[ACGT]AGG
3398 CCTTAGG
HhaI 43 GCGC GCG^C
2866 GCGC
HinP1I 43 GCGC G^CGC
2866 GCGC
MaeII 4429 ACGT A^CGT
4653 ACGT
MvnI 18 CGCG CG^CG
36 CGCG
NspI 1761 ACATGT [GA]CATG^[CT]
3089 ACATGC
PpuMI 4328 AGGACCC [GA]G^G[AT]CC[CT]
4641 AGGTCCT
SacI 1095 GAGCTC GAGCT^C
2719 GAGCTC
SapI 496 GCTCTTC GCTCTTC(1/4)
2854 GCTCTTC
SmlI 490 CTTGAG C^T[CT][GA]AG
4406 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanI BbsI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI