Restriction map for
pF1KE2659
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbeI BcgI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsgI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FseI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 73 GACGTC GACGT^C
AcsI 1164 AAATTT [GA]^AATT[CT]
AcyI 73 GACGTC G[GA]^CG[CT]C
ApaI 309 GGGCCC GGGCC^C
ApoI 1164 AAATTT [GA]^AATT[CT]
Asp700I 399 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 233 TGCGCA TGC^GCA
BamHI 227 GGATCC G^GATCC
BanI 327 GGCACC G^G[CT][GA]CC
BbrPI 507 CACGTG CAC^GTG
BbsI 1264 GTCTTC GAAGAC(2/6)
BglI 198 GCCGGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 401 AGATCT A^GATCT
BpmI 1086 CTGGAG CTGGAG(16/14)
BpuAI 1264 GTCTTC GAAGAC(2/6)
BsaHI 73 GACGTC G[GA]^CG[CT]C
BsaWI 69 ACCGGA [AT]^CCGG[AT]
BspDI 933 ATCGAT AT^CGAT
BspMI 1045 ACCTGC ACCTGC(4/8)
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BsrDI 45 CATTGC GCAATG(2/0)
BssSI 441 CTCGTG CACGAG(-5/-1)
BstEII 65 GGTCACC G^GT[ACGT]ACC
BstPI 65 GGTCACC G^GT[ACGT]ACC
BstXI 1330 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 933 ATCGAT AT^CGAT
DraII 1425 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 332 CGGCCG C^GGCCG
EclXI 332 CGGCCG C^GGCCG
Eco52I 332 CGGCCG C^GGCCG
EcoO109I 1425 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 65 GGTCACC G^GT[ACGT]ACC
FspI 233 TGCGCA TGC^GCA
HgaI 490 GACGC GACGC(5/10)
Hin1I 73 GACGTC G[GA]^CG[CT]C
HindIII 770 AAGCTT A^AGCTT
NaeI 346 GCCGGC GCC^GGC
NcoI 1108 CCATGG C^CATGG
NgoMIV 346 GCCGGC G^CCGGC
NspI 714 ACATGC [GA]CATG^[CT]
PflMI 518 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 507 CACGTG CAC^GTG
PmlI 507 CACGTG CAC^GTG
PpuMI 1425 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1397 CTGCAG CTGCA^G
SapI 1445 GAAGAGC GCTCTTC(1/4)
ScaI 125 AGTACT AGT^ACT
SfcI 1397 CTGCAG C^T[GA][CT]AG
SspI 1153 AATATT AAT^ATT
Tsp45I 66 GTCAC ^GT[GC]AC
Van91I 518 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 191 CCAGCGGGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 399 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 170 CGCG CG^CG
492 CGCG
AvaII 1275 GGTCC G^G[AT]CC
1426 GGACC
BbvCI 926 CCTCAGC CCTCAGC(-5/-2)
1215 CCTCAGC
BclI 1242 TGATCA T^GATCA
1352 TGATCA
BsaAI 507 CACGTG [CT]AC^GT[GA]
553 TACGTG
BsiEI 332 CGGCCG CG[GA][CT]^CG
419 CGGTCG
BsmFI 299 GGGAC GGGAC(10/14)
806 GGGAC
BstUI 170 CGCG CG^CG
492 CGCG
DsaI 176 CCGTGG C^C[GA][CT]GG
1108 CCATGG
EaeI 332 CGGCCG [CT]^GGCC[GA]
833 TGGCCG
EarI 1036 CTCTTC CTCTTC(1/4)
1445 GAAGAG
EcoNI 945 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1326 CCTGCCAGAGG
EcoT14I 662 CCAAGG C^C[AT][AT]GG
1108 CCATGG
FbaI 1242 TGATCA T^GATCA
1352 TGATCA
HaeII 290 AGCGCC [GA]GCGC^[CT]
1405 GGCGCT
Ksp632I 1036 CTCTTC CTCTTC(1/4)
1445 GAAGAG
MaeIII 66 GTCAC ^GT[ACGT]AC
1180 GTTAC
MvnI 170 CGCG CG^CG
492 CGCG
SacI 1089 GAGCTC GAGCT^C
1419 GAGCTC
SmlI 121 CTCAAG C^T[CT][GA]AG
394 CTCAAG
StyI 662 CCAAGG C^C[AT][AT]GG
1108 CCATGG
Tsp509I 1165 AATT ^AATT
1173 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
TaqI TfiI Tru9I TseI TspRI
TthHB8I XhoII XmaCI XmaI