Restriction map for
pF1KE2721
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssHII Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HindIII HinfI HpaI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspBI SspI StyI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 586 GGCGCC G[GA]^CG[CT]C
AlwNI 394 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 6 GGGCCC GGGCC^C
BanI 586 GGCGCC G^G[CT][GA]CC
BbeI 586 GGCGCC GGCGC^C
BbrPI 382 CACGTG CAC^GTG
BglI 268 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 24 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
BsaAI 382 CACGTG [CT]AC^GT[GA]
BsaHI 586 GGCGCC G[GA]^CG[CT]C
BsmFI 108 GTCCC GGGAC(10/14)
BspMI 308 ACCTGC ACCTGC(4/8)
BsrBI 27 GAGCGG CCGCTC(-3/-3)
BstXI 11 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 24 GCTGAGC GC^T[ACGT]AGC
DpnII 340 GATC ^GATC
DraII 6 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 519 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 6 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 29 GCGGG CCCGC(4/6)
Hin1I 586 GGCGCC G[GA]^CG[CT]C
KasI 586 GGCGCC G^GCGCC
MboI 340 GATC ^GATC
MseI 509 TTAA T^TAA
NaeI 199 GCCGGC GCC^GGC
NarI 586 GGCGCC GG^CGCC
NdeII 340 GATC ^GATC
NgoMIV 199 GCCGGC G^CCGGC
NspI 216 GCATGC [GA]CATG^[CT]
PmaCI 382 CACGTG CAC^GTG
PmlI 382 CACGTG CAC^GTG
PstI 310 CTGCAG CTGCA^G
SapI 48 GAAGAGC GCTCTTC(1/4)
Sau3AI 340 GATC ^GATC
SbfI 309 CCTGCAGG CCTGCA^GG
SfcI 310 CTGCAG C^T[GA][CT]AG
SfiI 267 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 586 GGCGCC GGC^GCC
SphI 216 GCATGC GCATG^C
Sse8387I 309 CCTGCAGG CCTGCA^GG
TaqI 212 TCGA T^CGA
Tru9I 509 TTAA T^TAA
Tsp45I 188 GTGAC ^GT[GC]AC
TthHB8I 212 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 51 GAGCAC G[AT]GC[AT]^C
79 GAGCAC
AvaI 126 CCCGAG C^[CT]CG[GA]G
228 CTCGGG
BanII 6 GGGCCC G[GA]GC[CT]^C
481 GAGCCC
BcnI 133 CCCGG CC^[GC]GG
484 CCCGG
BpmI 73 CTCCAG CTGGAG(16/14)
388 CTGGAG
BseRI 139 GAGGAG GAGGAG(10/8)
424 GAGGAG
BsiHKAI 51 GAGCAC G[AT]GC[AT]^C
79 GAGCAC
BsoBI 126 CCCGAG C^[CT]CG[GA]G
228 CTCGGG
BsrFI 199 GCCGGC [GA]^CCGG[CT]
583 ACCGGC
BssSI 117 CACGAG CACGAG(-5/-1)
246 CACGAG
Cfr10I 199 GCCGGC [GA]^CCGG[CT]
583 ACCGGC
DdeI 25 CTGAG C^T[ACGT]AG
557 CTGAG
EarI 48 GAAGAG CTCTTC(1/4)
63 GAAGAG
HaeII 549 GGCGCT [GA]GCGC^[CT]
586 GGCGCC
HphI 177 GGTGA GGTGA(8/7)
435 GGTGA
Ksp632I 48 GAAGAG CTCTTC(1/4)
63 GAAGAG
MaeII 302 ACGT A^CGT
383 ACGT
MaeIII 166 GTAAC ^GT[ACGT]AC
188 GTGAC
MboII 48 GAAGA GAAGA(8/7)
63 GAAGA
MslI 288 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
357 CATCTCTGTG
MspA1I 84 CAGCGG C[AC]G^C[GT]G
394 CAGCGG
NciI 133 CCCGG CC^[GC]GG
484 CCCGG
StuI 13 AGGCCT AGG^CCT
527 AGGCCT
TspRI 377 CACTG CA[GC]TG[ACGT][ACGT]^
414 CACTG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BssKI BstAPI BstNI Cac8I CfoI
Cfr13I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI