Restriction map for
pF1KE3718
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# NO CUT ENZYMES ( 52 enzymes) #
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AatII AccIII AclI AflII AgeI
Aor51HI AscI AvrII BfrI BlnI
BseAI BsiWI Bsp1407I BspEI BsrGI
BssHII BssSI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HpaI KspI MfeI MluI MroI
MunI NheI NotI NruI PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 2556 AAATTT [GA]^AATT[CT]
ApoI 2556 AAATTT [GA]^AATT[CT]
AviII 4730 TGCGCA TGC^GCA
BamHI 3055 GGATCC G^GATCC
BbrPI 264 CACGTG CAC^GTG
BclI 5888 TGATCA T^GATCA
BglII 6265 AGATCT A^GATCT
BsaBI 1362 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5621 ACCGGA [AT]^CCGG[AT]
BsmBI 5683 CGTCTC CGTCTC(1/5)
BspDI 1642 ATCGAT AT^CGAT
BspHI 6612 TCATGA T^CATGA
BsrBI 17 CCGCTC CCGCTC(-3/-3)
BsrFI 3893 GCCGGC [GA]^CCGG[CT]
Bst1107I 3842 GTATAC GTA^TAC
BstBI 2286 TTCGAA TT^CGAA
BstEII 7216 GGTCACC G^GT[ACGT]ACC
BstPI 7216 GGTCACC G^GT[ACGT]ACC
BstZ17I 3842 GTATAC GTA^TAC
Cfr10I 3893 GCCGGC [GA]^CCGG[CT]
ClaI 1642 ATCGAT AT^CGAT
DraI 2359 TTTAAA TTT^AAA
DrdI 7368 GACACACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 7216 GGTCACC G^GT[ACGT]ACC
FbaI 5888 TGATCA T^GATCA
FspI 4730 TGCGCA TGC^GCA
HindIII 6717 AAGCTT A^AGCTT
MamI 1362 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3893 GCCGGC GCC^GGC
NgoMIV 3893 GCCGGC G^CCGGC
NspV 2286 TTCGAA TT^CGAA
PflMI 6697 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 264 CACGTG CAC^GTG
PmlI 264 CACGTG CAC^GTG
PshAI 540 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 6612 TCATGA T^CATGA
SacI 2979 GAGCTC GAGCT^C
SapI 5938 GCTCTTC GCTCTTC(1/4)
SexAI 1871 ACCTGGT A^CC[AT]GGT
SfuI 2286 TTCGAA TT^CGAA
SmlI 6720 CTTGAG C^T[CT][GA]AG
Van91I 6697 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 3842 GTATAC GT^[AC][GT]AC
5587 GTCTAC
AccII 57 CGCG CG^CG
66 CGCG
AhdI 4117 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7267 GACCAGTGGTC
AseI 1930 ATTAAT AT^TAAT
1987 ATTAAT
AsnI 1930 ATTAAT AT^TAAT
1987 ATTAAT
AspEI 4117 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7267 GACCAGTGGTC
BbeI 1312 GGCGCC GGCGC^C
1801 GGCGCC
BbsI 5773 GTCTTC GAAGAC(2/6)
6131 GTCTTC
BcgI 3450 CGATGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4451 GCAACACGGTCG
BlpI 369 GCTCAGC GC^T[ACGT]AGC
7192 GCTGAGC
Bpu1102I 369 GCTCAGC GC^T[ACGT]AGC
7192 GCTGAGC
BpuAI 5773 GTCTTC GAAGAC(2/6)
6131 GTCTTC
BsaAI 264 CACGTG [CT]AC^GT[GA]
6474 CACGTA
BsiEI 1062 CGACCG CG[GA][CT]^CG
4457 CGGTCG
BstUI 57 CGCG CG^CG
66 CGCG
CelII 369 GCTCAGC GC^T[ACGT]AGC
7192 GCTGAGC
Eam1105I 4117 GACTGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7267 GACCAGTGGTC
KasI 1312 GGCGCC G^GCGCC
1801 GGCGCC
MvnI 57 CGCG CG^CG
66 CGCG
NarI 1312 GGCGCC GG^CGCC
1801 GGCGCC
NdeI 364 CATATG CA^TATG
6121 CATATG
PshBI 1930 ATTAAT AT^TAAT
1987 ATTAAT
SfoI 1312 GGCGCC GGC^GCC
1801 GGCGCC
XbaI 5808 TCTAGA T^CTAGA
6307 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp700I Asp718I AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SphI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmaCI XmaI XmnI