##################################
# NO CUT ENZYMES (  226 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        TseI        
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AccI           78  GTATAC               GT^[AC][GT]AC
AfaI          374  GTAC                 GT^AC
AluI          304  AGCT                 AG^CT
BsaJI         203  CCCAGG               C^C[ACGT][ACGT]GG
BsmAI         224  GAGAC                GTCTC(1/5)
BsmFI         201  GTCCC                GGGAC(10/14)
Bst1107I       78  GTATAC               GTA^TAC
BstZ17I        78  GTATAC               GTA^TAC
Cac8I          48  GCAAGC               GC[ACGT]^[ACGT]GC
EcoRV         163  GATATC               GAT^ATC
HaeIII         34  GGCC                 GG^CC
MaeIII        338  GTGAC                ^GT[ACGT]AC
MboII         183  TCTTC                GAAGA(8/7)
MnlI          382  CCTC                 CCTC(7/6)
MseI          301  TTAA                 T^TAA
NspI           40  GCATGT               [GA]CATG^[CT]
RsaI          374  GTAC                 GT^AC
SfaNI         257  GATGC                GCATC(5/9)
TaqI           92  TCGA                 T^CGA
Tru9I         301  TTAA                 T^TAA
Tsp45I        338  GTGAC                ^GT[GC]AC
TthHB8I        92  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BssKI         204  CCAGG                ^CC[ACGT]GG
              253  CCAGG               
BstF5I        207  GGATG                GGATG(2/0)
              256  GGATG               
BstNI         204  CCAGG                CC^[AT]GG
              253  CCAGG               
DdeI           74  CTGAG                C^T[ACGT]AG
              383  CTCAG               
EcoRII        204  CCAGG                ^CC[AT]GG
              253  CCAGG               
FokI          207  GGATG                GGATG(9/13)
              256  GGATG               
MvaI          204  CCAGG                CC^[AT]GG
              253  CCAGG               
ScrFI         204  CCAGG                CC^[ACGT]GG
              253  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

NlaIII      SfcI