##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        ApaLI       
ApoI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BamHI       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrGI       Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        Eam1105I    
Eco81I      EcoNI       EcoRI       EcoRV       FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MboI        MfeI        MflI        MluI        
MroI        MseI        MunI        NarI        NdeI        
NdeII       NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AfaI          353  GTAC                 GT^AC
AlwNI         595  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1141  AGCGCT               AGC^GCT
AscI          179  GGCGCGCC             GG^CGCGCC
BalI          437  TGGCCA               TGG^CCA
BbvCI         915  CCTCAGC              CCTCAGC(-5/-2)
BpmI          601  CTGGAG               CTGGAG(16/14)
BstEII        555  GGTGACC              G^GT[ACGT]ACC
BstPI         555  GGTGACC              G^GT[ACGT]ACC
EarI          483  GAAGAG               CTCTTC(1/4)
Eco47III     1141  AGCGCT               AGC^GCT
EcoO65I       555  GGTGACC              G^GT[ACGT]ACC
EcoT22I       844  ATGCAT               ATGCA^T
HgaI          303  GACGC                GACGC(5/10)
HinfI          22  GACTC                G^A[ACGT]TC
Ksp632I       483  GAAGAG               CTCTTC(1/4)
MluNI         437  TGGCCA               TGG^CCA
MscI          437  TGGCCA               TGG^CCA
NcoI         1073  CCATGG               C^CATGG
NsiI          844  ATGCAT               ATGCA^T
PleI           22  GACTC                GAGTC(4/5)
PpuMI         638  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         866  CAGCTG               CAG^CTG
RsaI          353  GTAC                 GT^AC
SacI          235  GAGCTC               GAGCT^C
SexAI         431  ACCAGGT              A^CC[AT]GGT
SfcI          879  CTACAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Asp700I       483  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              759  GAACCCATTC          
BanI          587  GGCACC               G^G[CT][GA]CC
              745  GGCACC              
BlpI          147  GCTGAGC              GC^T[ACGT]AGC
              397  GCTGAGC             
Bpu1102I      147  GCTGAGC              GC^T[ACGT]AGC
              397  GCTGAGC             
BspHI         422  TCATGA               T^CATGA
             1169  TCATGA              
BsrDI         377  GCAATG               GCAATG(2/0)
              407  GCAATG              
BssSI         900  CACGAG               CACGAG(-5/-1)
              238  CTCGTG              
BstXI         521  CCACCCAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1191  CCAGAGTGATGG        
CelII         147  GCTGAGC              GC^T[ACGT]AGC
              397  GCTGAGC             
EagI          161  CGGCCG               C^GGCCG
              416  CGGCCG              
EclXI         161  CGGCCG               C^GGCCG
              416  CGGCCG              
Eco52I        161  CGGCCG               C^GGCCG
              416  CGGCCG              
EcoT14I       741  CCAAGG               C^C[AT][AT]GG
             1073  CCATGG              
MaeII         212  ACGT                 A^CGT
              341  ACGT                
RcaI          422  TCATGA               T^CATGA
             1169  TCATGA              
StyI          741  CCAAGG               C^C[AT][AT]GG
             1073  CCATGG              
XmnI          483  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              759  GAACCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AspHI       
AvaI        AvaII       AviII       BanII       BbvI        
BcnI        BglI        BmyI        BsaJI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeIII      MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NgoMIV      NlaIII      NlaIV       
NspI        Sau96I      ScrFI       SfaNI       SmaI        
SphI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XmaCI       XmaI