Restriction map for
pF1KE2538
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# NO CUT ENZYMES ( 58 enzymes) #
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AatII Acc65I AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AviII AvrII BbeI BcgI
BciVI BfrI BglI BlnI BsaHI
BsiWI BssHII Bst1107I BstZ17I CpoI
DraIII Eco47III FseI FspI HaeII
Hin1I KasI KpnI KspI MluI
NarI NheI NotI NruI PacI
PflFI PinAI PmeI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 3420 TCCGGA T^CCGGA
AclI 4986 AACGTT AA^CGTT
AhdI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4336 GGGCCC GGGCC^C
AspEI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 6023 TGGCCA TGG^CCA
BbrPI 3887 CACGTG CAC^GTG
BbvCI 7081 CCTCAGC CCTCAGC(-5/-2)
BseAI 3420 TCCGGA T^CCGGA
BsiEI 5364 CGGCCG CG[GA][CT]^CG
BsmBI 3193 CGTCTC CGTCTC(1/5)
BspDI 1856 ATCGAT AT^CGAT
BspEI 3420 TCCGGA T^CCGGA
BsrBI 6265 GAGCGG CCGCTC(-3/-3)
BsrFI 6777 GCCGGC [GA]^CCGG[CT]
BssSI 1364 CACGAG CACGAG(-5/-1)
BstAPI 6414 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4713 TTCGAA TT^CGAA
CfoI 1332 GCGC GCG^C
Cfr10I 6777 GCCGGC [GA]^CCGG[CT]
ClaI 1856 ATCGAT AT^CGAT
DrdI 6896 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5364 CGGCCG C^GGCCG
Eam1105I 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5364 CGGCCG C^GGCCG
Eco52I 5364 CGGCCG C^GGCCG
EcoRV 4189 GATATC GAT^ATC
HhaI 1332 GCGC GCG^C
HinP1I 1332 GCGC G^CGC
HincII 4983 GTTAAC GT[CT]^[GA]AC
HindII 4983 GTTAAC GT[CT]^[GA]AC
HindIII 5383 AAGCTT A^AGCTT
HpaI 4983 GTTAAC GTT^AAC
MluNI 6023 TGGCCA TGG^CCA
MroI 3420 TCCGGA T^CCGGA
MscI 6023 TGGCCA TGG^CCA
NaeI 6777 GCCGGC GCC^GGC
NdeI 7328 CATATG CA^TATG
NgoMIV 6777 GCCGGC G^CCGGC
NspV 4713 TTCGAA TT^CGAA
PflMI 6714 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3887 CACGTG CAC^GTG
PmlI 3887 CACGTG CAC^GTG
Psp1406I 4986 AACGTT AA^CGTT
SfuI 4713 TTCGAA TT^CGAA
SpeI 2575 ACTAGT A^CTAGT
Van91I 6714 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1237 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 1331 CGCG CG^CG
5546 CGCG
AocI 1446 CCTAAGG CC^T[ACGT]AGG
7579 CCTGAGG
AseI 1807 ATTAAT AT^TAAT
5920 ATTAAT
AsnI 1807 ATTAAT AT^TAAT
5920 ATTAAT
Asp700I 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5028 GAAATGTTTC
AvaI 4772 CTCGAG C^[CT]CG[GA]G
7193 CTCGAG
BanI 172 GGCACC G^G[CT][GA]CC
6675 GGTGCC
BglII 706 AGATCT A^GATCT
1210 AGATCT
BsaWI 3420 TCCGGA [AT]^CCGG[AT]
7466 ACCGGA
BsmI 4487 GAATGC GAATGC(1/-1)
5803 GAATGC
BsoBI 4772 CTCGAG C^[CT]CG[GA]G
7193 CTCGAG
BstUI 1331 CGCG CG^CG
5546 CGCG
Bsu36I 1446 CCTAAGG CC^T[ACGT]AGG
7579 CCTGAGG
DsaI 3023 CCATGG C^C[GA][CT]GG
3236 CCATGG
Eco81I 1446 CCTAAGG CC^T[ACGT]AGG
7579 CCTGAGG
EcoT22I 2139 ATGCAT ATGCA^T
3050 ATGCAT
FauI 6477 CCCGC CCCGC(4/6)
6267 GCGGG
HgaI 1329 GACGC GACGC(5/10)
5869 GACGC
MfeI 5049 CAATTG C^AATTG
6953 CAATTG
MunI 5049 CAATTG C^AATTG
6953 CAATTG
MvnI 1331 CGCG CG^CG
5546 CGCG
NcoI 3023 CCATGG C^CATGG
3236 CCATGG
NsiI 2139 ATGCAT ATGCA^T
3050 ATGCAT
PaeR7I 4772 CTCGAG C^TCGAG
7193 CTCGAG
PpuMI 4364 GGGACCC [GA]G^G[AT]CC[CT]
7583 AGGACCT
PshAI 1619 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5493 GACTGCTGTC
PshBI 1807 ATTAAT AT^TAAT
5920 ATTAAT
PstI 10 CTGCAG CTGCA^G
377 CTGCAG
SexAI 5198 ACCAGGT A^CC[AT]GGT
6504 ACCAGGT
StuI 6521 AGGCCT AGG^CCT
7349 AGGCCT
XhoI 4772 CTCGAG C^TCGAG
7193 CTCGAG
XmnI 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5028 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BamHI BanII BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII